FastQCFastQC Report
Fri 10 Feb 2017
ERR1630251.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630251.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1506137
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54110.35926346673642573No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53150.3528895445766222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT36960.24539600315243568No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT20280.13464910562584945No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTAA701.21951605E-418.525
TCTTATA9700.017.54639237
ATTAGAC1352.2206223E-816.4444433
TACGGGT803.3834367E-416.187512
CTTATAC5450.015.95412837
TCGCGCG957.0619986E-515.5789489
TTAGACT1201.9363724E-615.4166674
GGTATCA29350.015.2538341
CCGCTAT855.365525E-415.23529325
ATCTCGC3200.015.03125111
TAACGCC1853.0559022E-1015.04
ACGGACC2350.014.9574478
GTTCTAG3350.014.9104481
TAATAGA1651.6458216E-814.5757574
TGTACTA1304.4458775E-614.2307695
ACGTCCG1051.6561689E-414.0952397
TTGTACT2252.7284841E-1113.97777754
TATTAGA2252.7284841E-1113.97777752
CGGTTCG1601.7842285E-713.87500122
TAGTGTC1203.3016957E-513.87500119
Produced by FastQC (version 0.11.5)