FastQCFastQC Report
Fri 10 Feb 2017
ERR1630250.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630250.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences39080
Sequences flagged as poor quality0
Sequence length43
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA2400.6141248720573182No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC2170.5552712384851587No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT1980.5066530194472876No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG1450.3710337768679631No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG1360.3480040941658137No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG1270.3249744114636643No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG1240.3172978505629478No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG1240.3172978505629478No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA1170.2993858751279427No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA1130.28915046059365407No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT1100.28147389969293757No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC1100.28147389969293757No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA1070.27379733879222107No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC980.2507676560900716No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG980.2507676560900716No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT970.24820880245649948No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC920.2354145342886387No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG920.2354145342886387No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA910.2328556806550665No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT880.22517911975435007No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC880.22517911975435007No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG830.21238485158648926No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA820.20982599795291706No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG810.20726714431934493No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG800.2047082906857728No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC780.19959058341862843No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA770.1970317297850563No Hit
CCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTCTG700.17911975435005117No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG670.1714431934493347No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG640.1637666325486182No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT640.1637666325486182No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC630.16120777891504606No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG630.16120777891504606No Hit
CCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGGC620.1586489252814739No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG610.15609007164790176No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG610.15609007164790176No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA610.15609007164790176No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC610.15609007164790176No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT590.15097236438075742No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC580.14841351074718526No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA580.14841351074718526No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC570.1458546571136131No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT570.1458546571136131No Hit
CGCTTGGTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGC560.14329580348004092No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG550.14073694984646878No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT540.13817809621289665No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA540.13817809621289665No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT530.13561924257932445No Hit
CTCCAGTGGGGCTCCCTGGGCACCCAGCAGTGGCTGTAGTAAC530.13561924257932445No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT530.13561924257932445No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGCTGT510.13050153531218014No Hit
CCGCTGGACTTATAATGCCACCTTCTGTCTCCTACGACTCCAT510.13050153531218014No Hit
GTCCAGAGTAAATGGGCACTAGACCAAGCGAGCACCTGCCTCA470.1202661207778915No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC470.1202661207778915No Hit
CTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTCCCATAC470.1202661207778915No Hit
ACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTATGC470.1202661207778915No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC470.1202661207778915No Hit
GACACAAAGAGGACACGCTGGCCTTCTCGGAGTGGGGGTCCCC450.11514841351074719No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG450.11514841351074719No Hit
TAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGGCAG440.11258955987717503No Hit
GAGCAGCAGGGAGAGGCAGAGGCGTGCGGCAGCCATCCGGAGT440.11258955987717503No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG440.11258955987717503No Hit
CCCGCATGCTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTA440.11258955987717503No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC430.11003070624360287No Hit
GTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGGAG420.10747185261003071No Hit
ATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTGT400.1023541453428864No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC502.6087946E-729.637
CCTACGA603.609087E-521.5833322
TACGACT603.609087E-521.5833324
ACGACTC603.609087E-521.5833325
GACTCCA603.609087E-521.5833327
CGACTCC603.609087E-521.5833326
TCCTACG656.68656E-519.9230771
ACTCCAT656.68656E-519.9230778
CTACGAC656.68656E-519.9230773
TCTTATA758.895944E-619.73333436
CTCCATG752.0036055E-417.2666669
TGCATAC700.002532872115.8571423
GGCCAGA700.002532872115.85714225
CATACTG700.002532872115.8571425
GCCAGAG750.004010786314.826
CTGCATA750.004010786314.82
AGCCAAG908.0245966E-414.38888931
CTCTTAT1051.5919856E-414.09523835
GTATGGG800.00615613313.8758
GGGTACA800.00615613313.8751
Produced by FastQC (version 0.11.5)