##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630250.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	39080
Sequences flagged as poor quality	0
Sequence length	43
%GC	52
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.670291709314228	31.0	31.0	34.0	30.0	34.0
2	31.849846468781987	33.0	31.0	34.0	30.0	34.0
3	32.00854657113613	33.0	31.0	34.0	30.0	34.0
4	35.702610030706246	37.0	35.0	37.0	33.0	37.0
5	35.60199590583419	37.0	35.0	37.0	33.0	37.0
6	35.6646110542477	37.0	35.0	37.0	33.0	37.0
7	35.642451381780965	37.0	35.0	37.0	33.0	37.0
8	35.61578812691914	37.0	35.0	37.0	33.0	37.0
9	37.28050153531218	39.0	37.0	39.0	34.0	39.0
10	37.23346980552712	39.0	37.0	39.0	34.0	39.0
11	37.3017911975435	39.0	37.0	39.0	34.0	39.0
12	37.17384851586489	39.0	37.0	39.0	33.0	39.0
13	37.248771750255884	39.0	37.0	39.0	34.0	39.0
14	38.40713920163767	40.0	38.0	41.0	34.0	41.0
15	38.416658137154556	40.0	38.0	41.0	34.0	41.0
16	38.38728249744115	40.0	38.0	41.0	34.0	41.0
17	38.364150460593656	40.0	38.0	41.0	33.0	41.0
18	38.3260747185261	40.0	38.0	41.0	33.0	41.0
19	38.30278915046059	40.0	38.0	41.0	34.0	41.0
20	38.2982088024565	40.0	38.0	41.0	34.0	41.0
21	38.238920163766636	40.0	38.0	41.0	33.0	41.0
22	38.240225179119754	40.0	38.0	41.0	33.0	41.0
23	38.23372569089048	40.0	38.0	41.0	34.0	41.0
24	38.18917604912999	40.0	38.0	41.0	33.0	41.0
25	38.14659672466735	40.0	38.0	41.0	33.0	41.0
26	37.97620266120778	40.0	37.0	41.0	33.0	41.0
27	37.86197543500512	40.0	37.0	41.0	33.0	41.0
28	37.79889969293757	40.0	37.0	41.0	32.0	41.0
29	37.76435516888434	40.0	37.0	41.0	32.0	41.0
30	37.723413510747186	40.0	37.0	41.0	33.0	41.0
31	37.62405322415558	40.0	37.0	41.0	32.0	41.0
32	37.56888433981576	40.0	37.0	41.0	32.0	41.0
33	37.513920163766635	40.0	37.0	41.0	32.0	41.0
34	37.4410440122825	40.0	37.0	41.0	31.0	41.0
35	37.31018423746162	39.0	36.0	41.0	31.0	41.0
36	37.19667349027635	39.0	36.0	41.0	31.0	41.0
37	37.13730808597748	39.0	36.0	41.0	31.0	41.0
38	37.062666325486184	39.0	36.0	41.0	30.0	41.0
39	36.94675025588536	39.0	36.0	41.0	30.0	41.0
40	36.824309109518936	39.0	36.0	41.0	30.0	41.0
41	36.718935516888436	39.0	35.0	40.0	30.0	41.0
42	36.723413510747186	39.0	35.0	40.0	30.0	41.0
43	35.441453428863866	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
19	2.0
20	4.0
21	13.0
22	18.0
23	43.0
24	71.0
25	103.0
26	182.0
27	250.0
28	377.0
29	485.0
30	698.0
31	837.0
32	1193.0
33	1476.0
34	1908.0
35	2553.0
36	3423.0
37	5041.0
38	9004.0
39	11399.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.26253838280451	18.705220061412486	12.59467758444217	25.437563971340836
2	19.682702149437052	19.524053224155576	30.93909928352098	29.854145342886383
3	22.23899692937564	20.278915046059364	28.82292732855681	28.65916069600819
4	16.376663254861825	13.50818833162743	32.126407369498466	37.98874104401228
5	15.834186284544524	35.16120777891504	34.16581371545548	14.838792221084955
6	38.856192425793246	32.272262026612076	13.134595701125896	15.73694984646878
7	31.734902763561923	28.8050153531218	18.950870010235416	20.50921187308086
8	31.34851586489253	29.552200614124875	18.272773797338793	20.826509723643806
9	26.392016376663257	14.754350051177074	18.664278403275333	40.18935516888434
10	20.046059365404297	26.49437052200614	30.135619242579324	23.323950870010236
11	37.12896622313204	21.476458546571138	19.178607983623337	22.21596724667349
12	25.478505629477993	24.59314227226203	24.490788126919142	25.437563971340836
13	33.88433981576254	17.497441146366427	22.732855680655067	25.88536335721597
14	24.56499488229273	20.03838280450358	22.36693961105425	33.02968270214944
15	27.45138178096213	28.162743091095187	19.641760491299898	24.744114636642784
16	26.507164790174002	25.028147389969295	21.9575230296827	26.507164790174002
17	26.489252814738997	24.675025588536336	22.428352098259978	26.40736949846469
18	24.150460593654046	24.01740020470829	25.266120777891505	26.56601842374616
19	28.034800409416583	23.037359263050153	25.61412487205732	23.313715455475947
20	28.930399181166838	23.009211873080858	22.13152507676561	25.928863868986696
21	26.916581371545544	22.37973387922211	24.644319344933468	26.059365404298873
22	26.98822927328557	23.23439099283521	24.76202661207779	25.015353121801432
23	26.53019447287615	22.899181166837256	23.275332650972363	27.295291709314228
24	25.632036847492323	23.751279426816787	25.867451381780963	24.749232343909927
25	26.3971340839304	24.019959058341865	23.09109518935517	26.491811668372566
26	26.686284544524053	24.163254861821905	24.053224155578302	25.097236438075743
27	25.79324462640737	22.233879222108495	23.89969293756397	28.073183213920167
28	25.143295803480044	24.247697031729786	22.935005117707266	27.674002047082908
29	26.658137154554762	24.651995905834188	24.106960081883315	24.58290685772774
30	27.49232343909928	22.566530194472875	22.12128966223132	27.81985670419652
31	23.817809621289662	25.445240532241552	25.670419651995907	25.066530194472875
32	24.877175025588535	23.193449334698055	24.362845445240534	27.566530194472875
33	23.825486182190378	25.340327533265096	24.506141248720574	26.328045035823948
34	27.79682702149437	21.545547594677583	23.840839303991814	26.816786079836234
35	24.91555783009212	22.778915046059364	24.022517911975434	28.283009211873082
36	23.467246673490276	24.199078812691916	26.31269191402252	26.02098259979529
37	25.232855680655064	21.568577277379735	27.075230296827023	26.123336745138175
38	26.218014329580345	22.827533265097237	24.4396110542477	26.51484135107472
39	24.396110542476972	21.47134083930399	27.256908904810643	26.875639713408393
40	23.24718526100307	20.386386898669397	28.303480040941658	28.062947799385874
41	21.484135107471854	21.775844421699077	29.127430910951897	27.612589559877176
42	22.697031729785056	20.79580348004094	29.87973387922211	26.627430910951894
43	23.710337768679633	21.507164790174002	28.574718526100305	26.20777891504606
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	4.0
1	5.0
2	6.0
3	8.5
4	11.0
5	11.0
6	16.5
7	22.0
8	23.5
9	25.0
10	29.5
11	34.0
12	34.0
13	70.0
14	106.0
15	152.0
16	198.0
17	188.5
18	179.0
19	179.0
20	221.5
21	264.0
22	272.5
23	281.0
24	318.0
25	355.0
26	355.0
27	397.5
28	440.0
29	527.0
30	614.0
31	719.0
32	824.0
33	824.0
34	907.0
35	990.0
36	1119.5
37	1249.0
38	1311.0
39	1373.0
40	1373.0
41	1464.5
42	1556.0
43	1641.5
44	1727.0
45	1855.5
46	1984.0
47	1984.0
48	2318.0
49	2652.0
50	2758.5
51	2865.0
52	2797.0
53	2729.0
54	2729.0
55	2523.5
56	2318.0
57	2575.5
58	2833.0
59	2930.0
60	3027.0
61	3027.0
62	2953.0
63	2879.0
64	2678.5
65	2478.0
66	2211.0
67	1944.0
68	1944.0
69	1879.0
70	1814.0
71	1375.0
72	936.0
73	565.0
74	194.0
75	194.0
76	136.0
77	78.0
78	62.5
79	47.0
80	35.5
81	24.0
82	24.0
83	18.5
84	13.0
85	9.0
86	5.0
87	2.5
88	0.0
89	0.0
90	1.0
91	2.0
92	1.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	39080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	62.53326509723644
#Duplication Level	Percentage of deduplicated	Percentage of total
1	89.47131516490711	55.949334698055274
2	5.561011539405843	6.95496417604913
3	1.5344954578934447	2.8787103377686796
4	0.7120058924625583	1.7809621289662232
5	0.4664866191996072	1.4585465711361312
6	0.3273590310172682	1.2282497441146365
7	0.16777150339634994	0.7343909928352098
8	0.1636795155086341	0.8188331627430911
9	0.13094361240690727	0.736949846468782
>10	1.2521482936410508	15.793244626407368
>50	0.15958752762091824	6.885875127942681
>100	0.05319584254030608	4.779938587512794
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	240	0.6141248720573182	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	217	0.5552712384851587	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	198	0.5066530194472876	No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG	145	0.3710337768679631	No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG	136	0.3480040941658137	No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG	127	0.3249744114636643	No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG	124	0.3172978505629478	No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG	124	0.3172978505629478	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	117	0.2993858751279427	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	113	0.28915046059365407	No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT	110	0.28147389969293757	No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC	110	0.28147389969293757	No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA	107	0.27379733879222107	No Hit
CCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCC	98	0.2507676560900716	No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG	98	0.2507676560900716	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	97	0.24820880245649948	No Hit
CCCCCACTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTC	92	0.2354145342886387	No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG	92	0.2354145342886387	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	91	0.2328556806550665	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	88	0.22517911975435007	No Hit
CATTTACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTC	88	0.22517911975435007	No Hit
TCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGG	83	0.21238485158648926	No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA	82	0.20982599795291706	No Hit
AGGCAGAGGCGTGCGGCAGCCATCCGGAGTCCAGAGTAAATGG	81	0.20726714431934493	No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG	80	0.2047082906857728	No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC	78	0.19959058341862843	No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA	77	0.1970317297850563	No Hit
CCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTCTG	70	0.17911975435005117	No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG	67	0.1714431934493347	No Hit
GCTCTGGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGG	64	0.1637666325486182	No Hit
TGCCACACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGT	64	0.1637666325486182	No Hit
GGTGTGGCATTGTCCCCTGGGTACACTGGCTCCAGTGGGGCTC	63	0.16120777891504606	No Hit
TCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTG	63	0.16120777891504606	No Hit
CCATGAGCAGCGCCAGCCCAGCTCTCCCCTCTGCACCCTTGGC	62	0.1586489252814739	No Hit
AGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCTCCCTG	61	0.15609007164790176	No Hit
TAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGG	61	0.15609007164790176	No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA	61	0.15609007164790176	No Hit
CCCTTGGCTCTGGCCAAAGCTTGCTCCCTGCTCCCACACAGGC	61	0.15609007164790176	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	59	0.15097236438075742	No Hit
GAGTGGGGGTCCCCGCATGCTGCTGTCCCCAGGGAGCTCAGCC	58	0.14841351074718526	No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA	58	0.14841351074718526	No Hit
GTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCACCTTC	57	0.1458546571136131	No Hit
GTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGAGCT	57	0.1458546571136131	No Hit
CGCTTGGTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGC	56	0.14329580348004092	No Hit
GTAGTAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAG	55	0.14073694984646878	No Hit
GGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGCCAGCGT	54	0.13817809621289665	No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA	54	0.13817809621289665	No Hit
GTCTCCTACGACTCCATGAGCAGCGCCAGCCCAGCTCTCCCCT	53	0.13561924257932445	No Hit
CTCCAGTGGGGCTCCCTGGGCACCCAGCAGTGGCTGTAGTAAC	53	0.13561924257932445	No Hit
GAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCAT	53	0.13561924257932445	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGCTGT	51	0.13050153531218014	No Hit
CCGCTGGACTTATAATGCCACCTTCTGTCTCCTACGACTCCAT	51	0.13050153531218014	No Hit
GTCCAGAGTAAATGGGCACTAGACCAAGCGAGCACCTGCCTCA	47	0.1202661207778915	No Hit
CCACTGGAGCCAGTGTACCCAGGGGACAATGCCACACCAGAGC	47	0.1202661207778915	No Hit
CTCCGAGAAGGCCAGCGTGTCCTCTTTGTGTCTTTTCCCATAC	47	0.1202661207778915	No Hit
ACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAGTATGC	47	0.1202661207778915	No Hit
GCTCTCCCCTCTGCACCCTTGGCTCTGGCCAAAGCTTGCTCCC	47	0.1202661207778915	No Hit
GACACAAAGAGGACACGCTGGCCTTCTCGGAGTGGGGGTCCCC	45	0.11514841351074719	No Hit
GCCTGAGGCAGGTGCTCGCTTGGTCTAGTGCCCATTTACTCTG	45	0.11514841351074719	No Hit
TAACAGAGCCACGCAGGTGGACAGGAGCAGCAGGGAGAGGCAG	44	0.11258955987717503	No Hit
GAGCAGCAGGGAGAGGCAGAGGCGTGCGGCAGCCATCCGGAGT	44	0.11258955987717503	No Hit
GAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACAGAAGG	44	0.11258955987717503	No Hit
CCCGCATGCTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTA	44	0.11258955987717503	No Hit
TATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGC	43	0.11003070624360287	No Hit
GTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGGAG	42	0.10747185261003071	No Hit
ATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTGT	40	0.1023541453428864	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.00255885363357216	0.0
10	0.0	0.0	0.0	0.00255885363357216	0.0
11	0.0	0.0	0.0	0.00255885363357216	0.0
12	0.0	0.0	0.0	0.00255885363357216	0.0
13	0.0	0.0	0.0	0.00255885363357216	0.0
14	0.0	0.0	0.0	0.00255885363357216	0.0
15	0.0	0.0	0.0	0.00255885363357216	0.0
16	0.0	0.0	0.0	0.00255885363357216	0.0
17	0.0	0.0	0.0	0.00255885363357216	0.0
18	0.0	0.0	0.0	0.00255885363357216	0.0
19	0.0	0.0	0.0	0.00255885363357216	0.0
20	0.0	0.0	0.0	0.00255885363357216	0.0
21	0.0	0.0	0.0	0.00255885363357216	0.0
22	0.0	0.0	0.0	0.00255885363357216	0.0
23	0.0	0.0	0.0	0.00255885363357216	0.0
24	0.0	0.0	0.0	0.00255885363357216	0.0
25	0.0	0.0	0.0	0.007676560900716479	0.0
26	0.0	0.0	0.0	0.0127942681678608	0.0
27	0.0	0.0	0.0	0.0255885363357216	0.0
28	0.0	0.0	0.0	0.11003070624360287	0.0
29	0.0	0.0	0.0	0.2507676560900716	0.0
30	0.0	0.0	0.0	0.3889457523029683	0.0
31	0.0	0.0	0.0	0.7446264073694985	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTATAC	50	2.6087946E-7	29.6	37
CCTACGA	60	3.609087E-5	21.583332	2
TACGACT	60	3.609087E-5	21.583332	4
ACGACTC	60	3.609087E-5	21.583332	5
GACTCCA	60	3.609087E-5	21.583332	7
CGACTCC	60	3.609087E-5	21.583332	6
TCCTACG	65	6.68656E-5	19.923077	1
ACTCCAT	65	6.68656E-5	19.923077	8
CTACGAC	65	6.68656E-5	19.923077	3
TCTTATA	75	8.895944E-6	19.733334	36
CTCCATG	75	2.0036055E-4	17.266666	9
TGCATAC	70	0.0025328721	15.857142	3
GGCCAGA	70	0.0025328721	15.857142	25
CATACTG	70	0.0025328721	15.857142	5
GCCAGAG	75	0.0040107863	14.8	26
CTGCATA	75	0.0040107863	14.8	2
AGCCAAG	90	8.0245966E-4	14.388889	31
CTCTTAT	105	1.5919856E-4	14.095238	35
GTATGGG	80	0.006156133	13.875	8
GGGTACA	80	0.006156133	13.875	1
>>END_MODULE