FastQCFastQC Report
Fri 10 Feb 2017
ERR1630249.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630249.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences630159
Sequences flagged as poor quality0
Sequence length43
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC64511.0237098890914833No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT33160.5262163993531791No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG20620.3272190034578575No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT20300.32214091999003425No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG19160.3040502476359141No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT19010.301669896010372No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG18640.2957983620007014No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT17090.2712013952034328No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG16840.2672341424941959No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT16560.2627908194598506No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG15980.25358679317442107No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT15660.24850870970659786No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC14580.23137017800269455No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG14200.22533995388465453No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG13780.21867496933313654No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG12920.2050276200133617No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC12910.20486892990499223No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT12680.2012190574124943No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT12350.19598228383630162No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG12030.19090420036847844No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA11940.18947598939315316No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT10700.16979841595533826No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA10660.16916365552186036No Hit
GCTCACACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACG10570.16773544454653508No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT10420.16535509292099296No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA10250.1626573610787119No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC9890.15694451717741079No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC9730.1544054754434992No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA9660.15329464468491286No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC9560.1517077436012181No Hit
GCCGCAGCCTTTGTGAACCAACACCTGTGCGGCTCACACCTGG9100.14440799861622228No Hit
CACCAGGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCG9070.14393192829111381No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG9070.14393192829111381No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT8940.14186895688231066No Hit
GTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTACTGCAA8940.14186895688231066No Hit
GGTGCAGGCAGCCTGCAGCCCTTGGCCCTGGAGGGGTCCCTGC8760.1390125349316601No Hit
GTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCCAA8540.13552135254753164No Hit
GGTAGAGGGAGCAGATGCTGGTACAGCATTGTTCCACAATGCC7890.12520649550351579No Hit
GTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGTTC7840.12441304496166841No Hit
GCCCAGCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGG7740.12282614387797365No Hit
CCATCAAGCAGATCACTGTCCTTCTGCCATGGCCCTGTGGATG7660.12155662301101784No Hit
CCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTCCAGCTGGTAGA7610.12076317246917048No Hit
GGGCTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCTCCACC7500.11901758127710627No Hit
CACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCTAGTGT7490.11885889116873677No Hit
GCTCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACAC7270.11536770878460834No Hit
TCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGCCC7260.11520901867623885No Hit
TTCTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGC7200.114256878026022No Hit
GCCATGGCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGC7130.11314604726743568No Hit
ACAGTGATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCCTG7080.1123525967255883No Hit
GTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCCTCTGG7010.11124176596700198No Hit
ACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCAAGAC6940.11013093520841565No Hit
GGCTGCGGCTGGGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGC6870.10902010444982933No Hit
CCCTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGC6820.10822665390798196No Hit
TTGTTCCACAATGCCACGCTTCTGCAGGGACCCCTCCAGGGCC6800.107909273691243No Hit
GATCACTGTCCTTCTGCCATGGCCCTGTGGATGCGCCTCCTGC6780.10759189347450407No Hit
GGTCAGGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAG6750.10711582314939563No Hit
CTGCAGCCCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCA6700.10632237260754826No Hit
GCTCCACCTGCCCCACCTGCAGGTCCTCTGCCTCCCGGCGGGG6660.10568761217407034No Hit
GGCAGGAGGCGCATCCACAGGGCCATGGCAGAAGGACAGTGAT6620.10505285174059245No Hit
CTCTACCAGCTGGAGAACTACTGCAACTAGACGCAGCCCGCAG6610.10489416163222298No Hit
GGACAGTGATCTGCTTGATGGCCTCTTCTGATGCAGCCTGTCC6610.10489416163222298No Hit
GTGGGGGGCTGCCTGCGGGCTGCGTCTAGTTGCAGTAGTTCTC6590.10457678141548403No Hit
GGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCTCTTCTG6460.10251381000668085No Hit
CTACTGCAACTAGACGCAGCCCGCAGGCAGCCCCCCACCCGCC6440.10219642978994191No Hit
GAGCAGATGCTGGTACAGCATTGTTCCACAATGCCACGCTTCT6380.10124428913972507No Hit
CCTCTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACAC6370.10108559903135557No Hit
GCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCGCCCAGCT6350.10076821881461664No Hit
GCCCTTGGCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTG6340.10060952870624715No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCATTAC405.935938E-527.751
GTAAACT509.08258E-625.9000024
GACAGGC14700.023.9115647
TGCATCA14550.023.7766314
TCCAGGA15050.023.4784072
CCAGGAC15450.023.4692553
CAGGACA15750.023.3746034
GCTGCAT15000.023.18666812
CTGCATC15350.022.53745813
GCATCAG15350.022.53745815
GGCCATC15200.022.51644726
ACAGGCT15600.022.1762838
ATCAGAA16000.021.73750117
AGGCCAT15850.021.70977825
GGACAGG16550.021.5740186
CATCAGA16050.021.55451816
GCCATCA16150.021.42105327
CCATCAA16050.021.32398828
CATCAAG16200.021.12654329
AAGAGGC16400.021.09451122
Produced by FastQC (version 0.11.5)