FastQCFastQC Report
Fri 10 Feb 2017
ERR1630248.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630248.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences744544
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT345734.643513345081017No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT251123.372802681910001No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT210042.821055572269738No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT94691.2717851463446082No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA44720.6006360940387674No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA32500.43650878927236003No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT30230.4060203292216444No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23170.3111971891520179No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA23140.31079425796192034No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19130.2569357888855461No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18200.2444449219925216No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA16960.22779043280182232No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA15930.21395646194180598No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13910.18682576180857008No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA13530.18172196673400096No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA13410.1801102419736107No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA13230.17769265483302532No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA12850.17258885975845617No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA12480.16761937508058625No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA12060.16197833841922035No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11010.14787574676580564No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10860.1458610908153178No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA10010.13444470709588688No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA9840.1321614303520007No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA9830.1320271199553015No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA9430.12665470408733398No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA9400.12625177289723644No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC9380.12598315210383806No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8860.11899901147548031No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG8760.11765590750848842No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA8510.11429814759100873No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA8290.1113433188636266No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA8020.10771693815274853No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA7890.10597090299565909No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7800.1047621094253664No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT7610.10221021188808184No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT401.5990663E-632.3756
CGTTAAC303.598272E-430.8333321
AGTCCTA850.030.47058929
ATTGCTC850.030.47058929
AACGTCA250.005495114729.628
ATTAACA250.005495114729.629
GTCAATG250.005495114729.631
CGTGTCA250.005495114729.610
AGGGGTG250.005495114729.65
ATGCCCC1750.028.54285837
CAATGCG1000.027.75000219
GAGTCCT1050.026.4285728
TGCGACG1100.025.22727222
TATTGTA901.4188117E-1024.6666685
GACGGTC451.3226001E-424.66666830
CGACGAG1200.024.66666624
TTAGAAT601.3363497E-624.6666663
ATCGTCA1150.024.13043610
CCAATGC1250.023.6818
GTCCTAC1103.6379788E-1223.54545430
Produced by FastQC (version 0.11.5)