##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630248.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	744544
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.761060461168178	31.0	31.0	34.0	30.0	34.0
2	31.82909136330425	31.0	31.0	34.0	30.0	34.0
3	31.863680319766193	33.0	31.0	34.0	30.0	34.0
4	35.60198457042163	37.0	35.0	37.0	33.0	37.0
5	35.58952862423175	37.0	35.0	37.0	33.0	37.0
6	35.72499140413461	37.0	35.0	37.0	33.0	37.0
7	35.676063469721065	37.0	35.0	37.0	33.0	37.0
8	35.743210609446855	37.0	35.0	37.0	33.0	37.0
9	37.345457085142044	39.0	37.0	39.0	34.0	39.0
10	37.24141622254695	39.0	37.0	39.0	34.0	39.0
11	37.37407057205484	39.0	37.0	39.0	34.0	39.0
12	37.26996121115744	39.0	37.0	39.0	34.0	39.0
13	37.34903914342201	39.0	37.0	39.0	34.0	39.0
14	38.257208438990844	40.0	38.0	41.0	34.0	41.0
15	38.225546106072976	40.0	38.0	41.0	33.0	41.0
16	38.13942627970946	40.0	37.0	41.0	33.0	41.0
17	38.335469764043495	40.0	38.0	41.0	34.0	41.0
18	38.475677730261744	40.0	38.0	41.0	34.0	41.0
19	38.54321839944986	40.0	38.0	41.0	34.0	41.0
20	38.60714074655091	40.0	38.0	41.0	34.0	41.0
21	38.59752815145915	40.0	38.0	41.0	34.0	41.0
22	38.55721354278592	40.0	38.0	41.0	34.0	41.0
23	38.47833036059655	40.0	38.0	41.0	34.0	41.0
24	38.43512001977049	40.0	38.0	41.0	34.0	41.0
25	38.380316005501356	40.0	38.0	41.0	34.0	41.0
26	38.1030886018825	40.0	37.0	41.0	34.0	41.0
27	37.91785441827481	40.0	37.0	41.0	33.0	41.0
28	37.76232029268922	40.0	36.0	41.0	33.0	41.0
29	37.536136212231916	39.0	36.0	41.0	33.0	41.0
30	37.221946587441444	39.0	35.0	41.0	33.0	41.0
31	36.82011808570078	38.0	35.0	40.0	32.0	41.0
32	36.501842738642715	38.0	35.0	40.0	31.0	41.0
33	36.276306034297505	38.0	35.0	40.0	31.0	41.0
34	36.03858066145184	38.0	35.0	40.0	31.0	41.0
35	35.80711012378046	38.0	35.0	40.0	30.0	41.0
36	35.48124758241286	37.0	35.0	40.0	30.0	41.0
37	35.18274541195685	37.0	35.0	40.0	28.0	41.0
38	34.85709105170413	36.0	34.0	40.0	26.0	41.0
39	34.48687787424249	36.0	34.0	40.0	24.0	41.0
40	34.071673400094554	35.0	33.0	40.0	22.0	41.0
41	33.584047416942454	35.0	33.0	40.0	19.0	41.0
42	33.12200756436154	35.0	33.0	40.0	15.0	41.0
43	32.00186019899429	35.0	31.0	39.0	10.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	12.0
11	16.0
12	17.0
13	14.0
14	10.0
15	7.0
16	12.0
17	6.0
18	22.0
19	47.0
20	151.0
21	292.0
22	539.0
23	1032.0
24	1693.0
25	2841.0
26	4433.0
27	6569.0
28	9585.0
29	12861.0
30	16804.0
31	21204.0
32	26601.0
33	34541.0
34	44560.0
35	57569.0
36	81486.0
37	140713.0
38	144630.0
39	136271.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.68819035543903	18.343039498001463	15.23966347187003	17.729106674689472
2	17.487885202217733	28.18127605621696	32.581284652082346	21.74955408948296
3	21.579517127261788	28.9519491124769	31.782943654102375	17.685590106158937
4	13.086533502385352	17.936078996002923	31.617204624575578	37.360182877036145
5	11.810181802552972	44.466545966390164	29.105868827094167	14.617403403962696
6	27.72690398418361	34.2933392788069	19.15346843168436	18.82628830532514
7	24.522929470924485	36.00646838870503	19.989282030343407	19.481320110027077
8	34.233705462672454	29.715503717711783	19.098804100227788	16.951986719387975
9	30.38826449477801	11.41208578673658	20.861762367301328	37.33788735118408
10	21.23514527012507	32.14867086431427	24.944664116559935	21.67151974900073
11	31.55407873812696	27.785329006747755	17.038482614862254	23.622109640263034
12	21.023069153737055	33.64582885632011	27.001224910817896	18.32987707912494
13	33.95111638801737	20.657476253921864	25.844275153651093	19.547132204409678
14	23.244966046331715	24.100120342115442	30.6876423690205	21.96727124253234
15	28.03205720548416	27.92326578415782	26.142578544719992	17.902098465638026
16	18.864163837194308	27.269711393819573	30.08217110070056	23.783953668285555
17	18.22592083207977	30.215675635019558	30.49651867451756	21.061884858383117
18	17.082133493789485	24.28385696479993	35.43605213392358	23.197957407487
19	17.433086560364465	28.71153350238535	37.66721644389049	16.188163493359696
20	19.242113293505824	25.492784845489318	38.578109554304376	16.686992306700475
21	20.59381312588645	25.816069970344262	36.32867903038638	17.261437873382903
22	20.613959685391325	26.97718334980874	34.279773928740276	18.129083036059658
23	18.635701852408992	28.25541539519491	34.85717432415009	18.251708428246012
24	17.448532255984873	29.318482185068984	35.57586925688744	17.65711630205871
25	17.522537284566123	29.335002363862984	35.4000569476082	17.742403403962694
26	18.418119009756307	29.37999634675721	35.043865775561954	17.15801886792453
27	17.15748162633773	30.055309021360728	35.41442816005501	17.372781192246528
28	16.07332810418189	31.687448962049253	35.57009390982937	16.669129023939487
29	16.41555099497142	31.762528473804103	35.2850872480337	16.536833283190784
30	15.549651867451756	33.53099346714231	36.34439334680019	14.57496131860575
31	16.350141831778913	32.30030730218765	34.85690570335668	16.49264516267675
32	14.622238578243865	33.1844726436584	35.398579533244515	16.794709244853227
33	15.584438260196846	32.4898192719302	36.100351355997766	15.825391111875186
34	14.662666007650321	33.17614539906305	34.82279086259509	17.338397730691536
35	15.595854643916276	33.932850174066274	34.535500924055526	15.935794257961922
36	14.85755039326084	34.008466927407916	34.35955430437959	16.774428374951647
37	15.372765074998926	34.44994520135815	33.789138049598144	16.388151674044785
38	15.261018824945202	33.185412816435296	34.17984699359608	17.373721365023425
39	15.538504104525725	32.4073526883569	35.06213198951305	16.992011217604333
40	14.995755791464305	33.00087033137061	34.648724588472945	17.35464928869214
41	14.53695147633988	32.58491103279323	35.02559956161087	17.852537929256027
42	14.210039970774059	32.632188292431344	34.97362143808828	18.18415029870632
43	14.185729788971505	33.063861907422535	34.482582627756045	18.267825675849917
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	111.0
1	208.5
2	306.0
3	1182.0
4	2058.0
5	2058.0
6	3108.0
7	4158.0
8	4041.0
9	3924.0
10	5728.0
11	7532.0
12	7532.0
13	15757.0
14	23982.0
15	42751.0
16	61520.0
17	51511.0
18	41502.0
19	41502.0
20	45284.0
21	49066.0
22	33998.5
23	18931.0
24	19010.0
25	19089.0
26	19089.0
27	19228.0
28	19367.0
29	18548.5
30	17730.0
31	17038.0
32	16346.0
33	16346.0
34	16748.0
35	17150.0
36	16905.5
37	16661.0
38	18716.5
39	20772.0
40	20772.0
41	24064.0
42	27356.0
43	31456.5
44	35557.0
45	42396.0
46	49235.0
47	49235.0
48	59107.5
49	68980.0
50	61118.0
51	53256.0
52	47858.5
53	42461.0
54	42461.0
55	39622.0
56	36783.0
57	33561.5
58	30340.0
59	25772.0
60	21204.0
61	21204.0
62	17606.0
63	14008.0
64	11301.0
65	8594.0
66	7440.0
67	6286.0
68	6286.0
69	5414.5
70	4543.0
71	3455.5
72	2368.0
73	1947.5
74	1527.0
75	1527.0
76	1229.0
77	931.0
78	706.0
79	481.0
80	364.0
81	247.0
82	247.0
83	176.5
84	106.0
85	75.5
86	45.0
87	35.0
88	25.0
89	25.0
90	15.0
91	5.0
92	2.5
93	0.0
94	0.5
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	744544.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	24.96526297210613
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.61758936155465	17.879519518395224
2	12.050447632562136	6.016851881570149
3	4.879284797599952	3.654378842636472
4	2.5650238997657255	2.561459847495541
5	1.5731451280491648	1.9636990907517484
6	1.1323921112763968	1.696228010733223
7	0.7909884391866656	1.3823064074533618
8	0.618015072541237	1.234312704537378
9	0.4767220691308316	1.0711342638411991
>10	3.4498132341382486	17.416309503874935
>50	0.48930175353853544	8.483347572253532
>100	0.3136273354178614	14.651795184537042
>500	0.0312549578251477	5.186764285271567
>1k	0.010238693080651833	4.672715773209974
>5k	5.388785831922017E-4	1.2738878201285593
>10k+	0.0016166357495766054	10.855289293310099
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	34573	4.643513345081017	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	25112	3.372802681910001	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	21004	2.821055572269738	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9469	1.2717851463446082	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	4472	0.6006360940387674	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	3250	0.43650878927236003	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3023	0.4060203292216444	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2317	0.3111971891520179	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	2314	0.31079425796192034	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1913	0.2569357888855461	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1820	0.2444449219925216	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	1696	0.22779043280182232	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	1593	0.21395646194180598	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1391	0.18682576180857008	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	1353	0.18172196673400096	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	1341	0.1801102419736107	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1323	0.17769265483302532	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	1285	0.17258885975845617	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	1248	0.16761937508058625	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	1206	0.16197833841922035	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1101	0.14787574676580564	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1086	0.1458610908153178	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1001	0.13444470709588688	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	984	0.1321614303520007	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	983	0.1320271199553015	No Hit
CCCATAGAGAGGGGAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	943	0.12665470408733398	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	940	0.12625177289723644	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	938	0.12598315210383806	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	886	0.11899901147548031	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	876	0.11765590750848842	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	851	0.11429814759100873	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	829	0.1113433188636266	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	802	0.10771693815274853	No Hit
GTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAA	789	0.10597090299565909	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	780	0.1047621094253664	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	761	0.10221021188808184	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.343103966991877E-4	0.0
3	0.0	0.0	0.0	2.686207933983754E-4	0.0
4	0.0	0.0	0.0	2.686207933983754E-4	0.0
5	0.0	0.0	0.0	2.686207933983754E-4	0.0
6	0.0	0.0	0.0	2.686207933983754E-4	0.0
7	0.0	0.0	0.0	2.686207933983754E-4	0.0
8	0.0	0.0	0.0	2.686207933983754E-4	0.0
9	0.0	0.0	0.0	4.0293119009756305E-4	0.0
10	0.0	0.0	0.0	4.0293119009756305E-4	0.0
11	0.0	0.0	0.0	6.715519834959385E-4	0.0
12	0.0	0.0	0.0	0.0010744831735935015	0.0
13	0.0	0.0	0.0	0.0012087935702926893	0.0
14	0.0	0.0	0.0	0.002148966347187003	0.0
15	0.0	0.0	0.0	0.00349207031417888	0.0
16	0.0	0.0	0.0	0.006849830231658572	0.0
17	0.0	0.0	0.0	0.011550694116130142	0.0
18	0.0	0.0	0.0	0.013565350066617956	0.0
19	0.0	0.0	0.0	0.017191730777496025	0.0
20	0.0	0.0	0.0	0.020549490694975716	0.0
21	0.0	0.0	0.0	0.02901104568702454	0.0
22	0.0	0.0	0.0	0.04083036059655306	0.0
23	0.0	0.0	0.0	0.05829071216744746	0.0
24	0.0	0.0	0.0	0.08663020587097606	0.0
25	0.0	0.0	0.0	0.09374865689603301	0.0
26	0.0	0.0	0.0	0.10677676537585422	0.0
27	0.0	0.0	0.0	0.12396849615335023	0.0
28	0.0	0.0	0.0	0.15808333691494392	0.0
29	0.0	0.0	0.0	0.21341922035500924	0.0
30	0.0	0.0	0.0	0.3321496110370912	0.0
31	0.0	0.0	0.0	0.6307216228993854	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGACCGT	40	1.5990663E-6	32.375	6
CGTTAAC	30	3.598272E-4	30.833332	1
AGTCCTA	85	0.0	30.470589	29
ATTGCTC	85	0.0	30.470589	29
AACGTCA	25	0.0054951147	29.6	28
ATTAACA	25	0.0054951147	29.6	29
GTCAATG	25	0.0054951147	29.6	31
CGTGTCA	25	0.0054951147	29.6	10
AGGGGTG	25	0.0054951147	29.6	5
ATGCCCC	175	0.0	28.542858	37
CAATGCG	100	0.0	27.750002	19
GAGTCCT	105	0.0	26.42857	28
TGCGACG	110	0.0	25.227272	22
TATTGTA	90	1.4188117E-10	24.666668	5
GACGGTC	45	1.3226001E-4	24.666668	30
CGACGAG	120	0.0	24.666666	24
TTAGAAT	60	1.3363497E-6	24.666666	3
ATCGTCA	115	0.0	24.130436	10
CCAATGC	125	0.0	23.68	18
GTCCTAC	110	3.6379788E-12	23.545454	30
>>END_MODULE