FastQCFastQC Report
Fri 10 Feb 2017
ERR1630247.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630247.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences51319
Sequences flagged as poor quality0
Sequence length43
%GC38

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT26805.222237377969173No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT13482.626707457277032No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT12662.4669225822794676No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7051.3737602057717413No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6391.2451528673590677No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA3600.7014945731600382No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2650.5163779496872504No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2250.4384341082250239No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1880.3663360548724644No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1820.3546444786531304No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1690.3293127301779068No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1320.2572146768253473No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1270.24747169664256904No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1190.23188292835012372No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1120.2182427560942341No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA1060.20655117987490013No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA960.18706521950934352No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA940.1831680274362322No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT920.17927083536312088No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA910.1773222393265652No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA900.17537364329000954No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA820.15978487499756425No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA820.15978487499756425No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA820.15978487499756425No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA820.15978487499756425No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACCTGT790.1539390868878973No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA770.15004189481478594No Hit
GTTGATACCTCTGCTTCCCATGTACTCTGCGTTGATACCCTGT740.14419610670511895No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT740.14419610670511895No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA730.1422475106685633No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA700.13640172255889632No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT690.13445312652234065No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA690.13445312652234065No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG660.12860733841267366No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA630.12276155030300669No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA630.12276155030300669No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12081295426645101No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA620.12081295426645101No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA620.12081295426645101No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA610.11886435822989537No Hit
TATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACCTGTCT590.11496716615678404No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT580.11301857012022837No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA570.11106997408367272No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA570.11106997408367272No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA540.10522418597400574No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA520.10132699390089439No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT520.10132699390089439No Hit
GAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA520.10132699390089439No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG520.10132699390089439No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATTGT200.001825594837.010
CAACTGT251.2172615E-436.99999635
TCAACTG251.2172615E-436.99999634
ATCAACT251.2172615E-436.99999633
CGCCTTT451.0461554E-732.8888914
CCAGACG451.0461554E-732.8888921
GCGCGCC451.0461554E-732.8888911
ACGCTTC352.3444512E-531.71428525
TCCAGAC551.783701E-830.27272820
GCCTTTC502.6361158E-729.59999815
TGTCTGA250.005448139329.59999810
TCGCGCG502.6361158E-729.5999989
GTCTGAA250.005448139329.59999811
GGGGGAC250.005448139329.59999822
GCGCCTT502.6361158E-729.59999813
AGACGCT502.6361158E-729.59999823
CTGTCTG250.005448139329.5999989
TGAATAT250.005448139329.59999814
CGCGCGC502.6361158E-729.59999810
TATGGGG250.005448139329.59999818
Produced by FastQC (version 0.11.5)