##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630247.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	51319
Sequences flagged as poor quality	0
Sequence length	43
%GC	38
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.36421208519262	31.0	31.0	34.0	30.0	34.0
2	31.39353845554278	31.0	31.0	34.0	28.0	34.0
3	31.371246516884586	31.0	31.0	34.0	28.0	34.0
4	35.249712582084605	37.0	35.0	37.0	33.0	37.0
5	35.23856661275551	37.0	35.0	37.0	33.0	37.0
6	35.379001929110075	37.0	35.0	37.0	33.0	37.0
7	35.312126113135484	37.0	35.0	37.0	33.0	37.0
8	35.392154952356826	37.0	35.0	37.0	33.0	37.0
9	36.912215748553166	39.0	37.0	39.0	33.0	39.0
10	36.744383171924625	39.0	37.0	39.0	32.0	39.0
11	36.89148268672422	39.0	37.0	39.0	33.0	39.0
12	36.790935131237944	39.0	37.0	39.0	32.0	39.0
13	36.9022973947271	39.0	37.0	39.0	33.0	39.0
14	37.590093337750154	39.0	37.0	41.0	32.0	41.0
15	37.59383464214034	39.0	37.0	41.0	32.0	41.0
16	37.50529043823925	39.0	36.0	40.0	32.0	41.0
17	37.783335606695374	39.0	37.0	40.0	33.0	41.0
18	37.95159687445196	39.0	37.0	41.0	33.0	41.0
19	38.00280597829264	40.0	37.0	41.0	33.0	41.0
20	38.05543755724001	40.0	37.0	41.0	33.0	41.0
21	38.03184005923732	40.0	37.0	41.0	33.0	41.0
22	37.98711978019837	40.0	37.0	41.0	33.0	41.0
23	37.94284767824782	40.0	37.0	41.0	33.0	41.0
24	37.88902745571816	40.0	37.0	41.0	33.0	41.0
25	37.82903018375261	40.0	37.0	41.0	33.0	41.0
26	37.53627311522048	39.0	36.0	41.0	32.0	41.0
27	37.27864923322746	39.0	36.0	41.0	32.0	41.0
28	37.10450320544048	39.0	35.0	40.0	32.0	41.0
29	36.839747461953664	39.0	35.0	40.0	31.0	41.0
30	36.49769091369668	38.0	35.0	40.0	31.0	41.0
31	36.0603675052125	38.0	35.0	40.0	30.0	41.0
32	35.741109530583216	37.0	35.0	40.0	30.0	41.0
33	35.507161090434344	37.0	34.0	40.0	30.0	41.0
34	35.33504160252538	37.0	34.0	40.0	30.0	41.0
35	35.15029521229954	36.0	34.0	40.0	29.0	41.0
36	34.8267698123502	36.0	34.0	40.0	26.0	41.0
37	34.54997174535747	36.0	33.0	40.0	25.0	41.0
38	34.23311054385315	35.0	33.0	40.0	24.0	41.0
39	33.90290145949843	35.0	33.0	40.0	22.0	41.0
40	33.45602993043512	35.0	33.0	40.0	20.0	41.0
41	32.899822677760675	35.0	33.0	40.0	16.0	41.0
42	32.37720922075645	35.0	32.0	39.0	12.0	41.0
43	31.201173054814006	35.0	30.0	38.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	0.0
13	1.0
14	1.0
15	0.0
16	0.0
17	2.0
18	2.0
19	5.0
20	16.0
21	28.0
22	54.0
23	117.0
24	189.0
25	269.0
26	430.0
27	625.0
28	881.0
29	1114.0
30	1487.0
31	1800.0
32	2188.0
33	2858.0
34	3660.0
35	4544.0
36	6318.0
37	9413.0
38	9723.0
39	5591.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.83376527212144	17.233383347298272	16.970322882363256	16.962528498217036
2	18.008924569847427	31.023597498002687	30.653364251057113	20.31411368109277
3	20.610300278649234	29.811570763265067	34.657729106179	14.920399851906701
4	13.741499249790525	18.46489604240145	31.07231239891658	36.72129230889144
5	11.886435822989535	43.61542508622537	29.74726709405873	14.750871996726358
6	27.81815701786863	35.88729320524562	17.685457627779186	18.60909214910657
7	20.89869249205947	35.92236793390362	21.98016329234786	21.198776281689042
8	33.44570237144138	30.71182213215378	19.380736179582613	16.46173931682223
9	27.837642978234182	11.186889845866054	20.723318848769463	40.25214832713031
10	20.388160330481888	32.4207408562131	24.170385237436427	23.020713575868587
11	30.121397533077417	28.149418344083088	16.313646018043997	25.415538104795495
12	18.930610495138254	36.69790915645277	26.820475847152125	17.551004501256845
13	34.72982715953156	19.73538065823574	25.472047389855607	20.062744792377092
14	23.0246107679417	25.088173970654143	30.497476568132658	21.389738693271497
15	26.304097897464878	29.503692589489273	25.84422923283774	18.34798028020811
16	16.077865897620764	28.675539273953117	29.653734484304056	25.59286034412206
17	16.970322882363256	32.34474561078743	29.605019583390167	21.079911923459147
18	15.156179972329936	24.647791266392563	35.56577485921394	24.63025390206356
19	16.791052047000136	30.240261891307313	36.3101385451782	16.658547516514354
20	18.587657592704456	27.22773241879226	38.46138856953565	15.723221418967634
21	22.640737348740235	26.838013211481126	35.08447163818469	15.436777801593951
22	21.615775833511954	28.932753950778462	32.86307215651124	16.588398059198347
23	19.337867066778387	30.07657982423664	34.00884662600596	16.576706482979013
24	17.157388101872602	31.894619926343072	34.827256961359346	16.120735010424987
25	16.235702176581775	31.228200081841035	34.870126074163565	17.66597166741363
26	19.11182992653793	29.905103373019738	34.57783666868022	16.405230031762116
27	17.038523743642706	31.031391882148913	34.460920906486876	17.469163467721508
28	16.239599368654883	32.486993121455995	36.05682106042597	15.216586449463163
29	18.071279643017206	31.748475223601396	35.222821956780145	14.957423176601258
30	16.520197197918897	33.51000604064771	35.45080769305715	14.518989068376234
31	17.410705586624836	31.80498450866151	34.75710750404334	16.02720240067032
32	14.232545451002554	33.367758529979156	35.5014711900076	16.898224829010697
33	14.20721370252733	32.80266567937801	37.771585572594944	15.218535045499717
34	14.113681092772657	33.5587209415616	35.14292951928136	17.18466844638438
35	14.160447397649994	34.568093688497434	35.19749020830491	16.073968705547653
36	13.486233169001736	34.08873906350475	34.08484187143163	18.340185896061886
37	14.020148483017985	34.30308462752586	34.29918743545276	17.377579454003392
38	14.010405502835207	32.94491319004657	34.2446267464292	18.800054560689023
39	13.866209396130088	31.54971842787272	36.60242795066155	17.981644225335646
40	14.201367914417663	32.259007385178975	36.64139987139266	16.898224829010697
41	13.76683099826575	31.993998324207407	36.89861454821801	17.340556129308833
42	13.131588690348602	32.048559013230964	37.05255363510591	17.767298661314523
43	12.584033204076464	32.64288080438044	36.59463356651533	18.178452425027768
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	3.0
1	9.0
2	15.0
3	79.0
4	143.0
5	143.0
6	221.5
7	300.0
8	321.5
9	343.0
10	452.0
11	561.0
12	561.0
13	1211.5
14	1862.0
15	3559.5
16	5257.0
17	4094.0
18	2931.0
19	2931.0
20	3075.0
21	3219.0
22	2136.5
23	1054.0
24	1015.0
25	976.0
26	976.0
27	940.5
28	905.0
29	909.0
30	913.0
31	933.5
32	954.0
33	954.0
34	974.5
35	995.0
36	1005.0
37	1015.0
38	1198.0
39	1381.0
40	1381.0
41	1786.0
42	2191.0
43	2542.5
44	2894.0
45	3668.5
46	4443.0
47	4443.0
48	4962.5
49	5482.0
50	4473.0
51	3464.0
52	3080.5
53	2697.0
54	2697.0
55	2493.5
56	2290.0
57	2065.5
58	1841.0
59	1562.5
60	1284.0
61	1284.0
62	1059.5
63	835.0
64	637.5
65	440.0
66	356.0
67	272.0
68	272.0
69	225.5
70	179.0
71	134.0
72	89.0
73	66.0
74	43.0
75	43.0
76	32.5
77	22.0
78	19.5
79	17.0
80	10.0
81	3.0
82	3.0
83	2.0
84	1.0
85	3.0
86	5.0
87	2.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	51319.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	43.742083828601494
#Duplication Level	Percentage of deduplicated	Percentage of total
1	77.94458303635068	34.094584851614414
2	10.009800427655025	8.756990588281145
3	3.96471846044191	5.202751417603617
4	2.1872772630078403	3.8270426157953197
5	1.2963292943692089	2.8352072331884877
6	0.9310406272273699	2.4435394298407997
7	0.565751960085531	1.7323018764979832
8	0.4632929436920884	1.6212319024143103
9	0.31628652886671416	1.2451528673590677
>10	2.0937277263007843	16.742337146086246
>50	0.15591589451176052	4.696116448099144
>100	0.049002138275124736	3.867963132562988
>500	0.008909479686386316	2.618913073130809
>1k	0.013364219529579474	10.315867417525673
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	2680	5.222237377969173	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1348	2.626707457277032	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1266	2.4669225822794676	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	705	1.3737602057717413	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	639	1.2451528673590677	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	360	0.7014945731600382	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	265	0.5163779496872504	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.4384341082250239	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	188	0.3663360548724644	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	182	0.3546444786531304	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	169	0.3293127301779068	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	132	0.2572146768253473	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	127	0.24747169664256904	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	119	0.23188292835012372	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	112	0.2182427560942341	No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	106	0.20655117987490013	No Hit
GAATGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	96	0.18706521950934352	No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	94	0.1831680274362322	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	92	0.17927083536312088	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	91	0.1773222393265652	No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	90	0.17537364329000954	No Hit
GAACATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.15978487499756425	No Hit
GTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	82	0.15978487499756425	No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA	82	0.15978487499756425	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	82	0.15978487499756425	No Hit
GGTATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACCTGT	79	0.1539390868878973	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	77	0.15004189481478594	No Hit
GTTGATACCTCTGCTTCCCATGTACTCTGCGTTGATACCCTGT	74	0.14419610670511895	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	74	0.14419610670511895	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	73	0.1422475106685633	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	70	0.13640172255889632	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT	69	0.13445312652234065	No Hit
CAGTTGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	69	0.13445312652234065	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	66	0.12860733841267366	No Hit
GAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAA	63	0.12276155030300669	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	63	0.12276155030300669	No Hit
CAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.12081295426645101	No Hit
GGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	62	0.12081295426645101	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	62	0.12081295426645101	No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA	61	0.11886435822989537	No Hit
TATCAACGCAGAGTACATGGGAAGCAGAGGTATCAACCTGTCT	59	0.11496716615678404	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGTT	58	0.11301857012022837	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	57	0.11106997408367272	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	57	0.11106997408367272	No Hit
GTACATGGGAAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAA	54	0.10522418597400574	No Hit
AAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.10132699390089439	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	52	0.10132699390089439	No Hit
GAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	52	0.10132699390089439	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	52	0.10132699390089439	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0019485960365556616	0.0
11	0.0	0.0	0.0	0.0038971920731113233	0.0
12	0.0	0.0	0.0	0.0038971920731113233	0.0
13	0.0	0.0	0.0	0.005845788109666985	0.0
14	0.0	0.0	0.0	0.0077943841462226465	0.0
15	0.0	0.0	0.0	0.0077943841462226465	0.0
16	0.0	0.0	0.0	0.019485960365556616	0.0
17	0.0	0.0	0.0	0.031177536584890586	0.0
18	0.0	0.0	0.0	0.031177536584890586	0.0
19	0.0	0.0	0.0	0.042869112804224556	0.0
20	0.0	0.0	0.0	0.05845788109666985	0.0
21	0.0	0.0	0.0	0.0818410335353378	0.0
22	0.0	0.0	0.0	0.13835031859545197	0.0
23	0.0	0.0	0.0	0.1890138155458992	0.0
24	0.0	0.0	0.0	0.2494202926791247	0.0
25	0.0	0.0	0.0	0.28644361737368224	0.0
26	0.0	0.0	0.0	0.3195697499951285	0.0
27	0.0	0.0	0.0	0.35269588261657475	0.0
28	0.0	0.0	0.0	0.3955649954207993	0.0
29	0.0	0.0	0.0	0.5163779496872504	0.0
30	0.0	0.0	0.0	0.9431204816929403	0.0
31	0.0	0.0	0.0	1.734250472534539	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGATTGT	20	0.0018255948	37.0	10
CAACTGT	25	1.2172615E-4	36.999996	35
TCAACTG	25	1.2172615E-4	36.999996	34
ATCAACT	25	1.2172615E-4	36.999996	33
CGCCTTT	45	1.0461554E-7	32.88889	14
CCAGACG	45	1.0461554E-7	32.88889	21
GCGCGCC	45	1.0461554E-7	32.88889	11
ACGCTTC	35	2.3444512E-5	31.714285	25
TCCAGAC	55	1.783701E-8	30.272728	20
GCCTTTC	50	2.6361158E-7	29.599998	15
TGTCTGA	25	0.0054481393	29.599998	10
TCGCGCG	50	2.6361158E-7	29.599998	9
GTCTGAA	25	0.0054481393	29.599998	11
GGGGGAC	25	0.0054481393	29.599998	22
GCGCCTT	50	2.6361158E-7	29.599998	13
AGACGCT	50	2.6361158E-7	29.599998	23
CTGTCTG	25	0.0054481393	29.599998	9
TGAATAT	25	0.0054481393	29.599998	14
CGCGCGC	50	2.6361158E-7	29.599998	10
TATGGGG	25	0.0054481393	29.599998	18
>>END_MODULE