FastQCFastQC Report
Fri 10 Feb 2017
ERR1630246.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630246.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences262144
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53962.05841064453125No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT39651.5125274658203125No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT27541.050567626953125No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14610.5573272705078125No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6410.2445220947265625No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5690.2170562744140625No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5610.2140045166015625No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA5420.206756591796875No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5290.2017974853515625No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC5130.1956939697265625No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5130.1956939697265625No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA5040.1922607421875No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4820.183868408203125No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4660.177764892578125No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC4480.1708984375No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4420.168609619140625No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4360.16632080078125No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4340.165557861328125No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3970.1514434814453125No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3890.1483917236328125No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3880.14801025390625No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3740.142669677734375No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3720.14190673828125No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3460.131988525390625No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3420.130462646484375No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3370.1285552978515625No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT3340.127410888671875No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG3240.12359619140625No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG3220.122833251953125No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG3150.1201629638671875No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3100.118255615234375No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3080.11749267578125No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC3000.11444091796875No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2840.10833740234375No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2830.1079559326171875No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2760.10528564453125No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2740.104522705078125No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2720.103759765625No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2690.1026153564453125No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2650.1010894775390625No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC2640.1007080078125No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATAATCC250.005488701629.5999983
CATCTGT250.005488701629.59999837
GTAATCT250.005488701629.59999836
TTGGATA405.9229154E-527.7514
AATCCTC358.8510616E-426.4285725
TAATCCT358.8510616E-426.4285724
CTAGATG451.3194082E-424.6666683
CTATCTT451.3194082E-424.6666689
CGCACTA451.3194082E-424.66666829
ATACCAT551.895403E-523.5454546
ACCATGC400.001927233823.1258
AACCAGA400.001927233823.1251
CAGGCAG400.001927233823.12510
GTATACT400.001927233823.1254
TCTTGGA502.694398E-422.19999912
ACACGTT502.694398E-422.19999914
ACGTTTC502.694398E-422.19999916
ATATGGC502.694398E-422.19999923
TATCTTG502.694398E-422.19999910
TGAATGG502.694398E-422.19999929
Produced by FastQC (version 0.11.5)