##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630246.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	262144
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94546890258789	33.0	31.0	34.0	30.0	34.0
2	32.06550979614258	33.0	31.0	34.0	30.0	34.0
3	32.138755798339844	34.0	31.0	34.0	30.0	34.0
4	35.800628662109375	37.0	35.0	37.0	35.0	37.0
5	35.775875091552734	37.0	35.0	37.0	35.0	37.0
6	35.87108612060547	37.0	35.0	37.0	35.0	37.0
7	35.8554801940918	37.0	35.0	37.0	35.0	37.0
8	35.88969421386719	37.0	35.0	37.0	35.0	37.0
9	37.551422119140625	39.0	37.0	39.0	35.0	39.0
10	37.493629455566406	39.0	37.0	39.0	35.0	39.0
11	37.59568786621094	39.0	37.0	39.0	35.0	39.0
12	37.48356246948242	39.0	37.0	39.0	35.0	39.0
13	37.55986022949219	39.0	37.0	39.0	35.0	39.0
14	38.67110061645508	40.0	38.0	41.0	35.0	41.0
15	38.68463134765625	40.0	38.0	41.0	35.0	41.0
16	38.553531646728516	40.0	38.0	41.0	34.0	41.0
17	38.66323471069336	40.0	38.0	41.0	35.0	41.0
18	38.68392562866211	40.0	38.0	41.0	35.0	41.0
19	38.70432662963867	40.0	38.0	41.0	35.0	41.0
20	38.69834518432617	40.0	38.0	41.0	35.0	41.0
21	38.67060852050781	40.0	38.0	41.0	35.0	41.0
22	38.61891555786133	40.0	38.0	41.0	34.0	41.0
23	38.56538391113281	40.0	38.0	41.0	34.0	41.0
24	38.50434875488281	40.0	38.0	41.0	34.0	41.0
25	38.478214263916016	40.0	38.0	41.0	34.0	41.0
26	38.2298469543457	40.0	37.0	41.0	34.0	41.0
27	38.07468032836914	40.0	37.0	41.0	33.0	41.0
28	37.979339599609375	40.0	37.0	41.0	33.0	41.0
29	37.86923599243164	40.0	37.0	41.0	33.0	41.0
30	37.67893600463867	39.0	36.0	41.0	33.0	41.0
31	37.45777893066406	39.0	36.0	41.0	33.0	41.0
32	37.282188415527344	39.0	35.0	41.0	33.0	41.0
33	37.13023376464844	39.0	35.0	41.0	33.0	41.0
34	36.94967269897461	39.0	35.0	41.0	32.0	41.0
35	36.79425811767578	39.0	35.0	41.0	31.0	41.0
36	36.56898880004883	39.0	35.0	41.0	31.0	41.0
37	36.37221145629883	39.0	35.0	40.0	31.0	41.0
38	36.1364860534668	38.0	35.0	40.0	30.0	41.0
39	35.91392517089844	38.0	35.0	40.0	29.0	41.0
40	35.660770416259766	38.0	35.0	40.0	28.0	41.0
41	35.34712600708008	38.0	35.0	40.0	26.0	41.0
42	35.08816146850586	38.0	35.0	40.0	25.0	41.0
43	33.95956802368164	37.0	33.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	3.0
11	1.0
12	2.0
13	4.0
14	0.0
15	1.0
16	0.0
17	2.0
18	6.0
19	12.0
20	32.0
21	74.0
22	155.0
23	263.0
24	425.0
25	754.0
26	1202.0
27	1689.0
28	2487.0
29	3362.0
30	4392.0
31	5877.0
32	7557.0
33	9733.0
34	13069.0
35	17515.0
36	24947.0
37	41190.0
38	59857.0
39	67533.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.43626403808594	20.964813232421875	13.293838500976562	21.305084228515625
2	19.356155395507812	23.579025268554688	33.385467529296875	23.679351806640625
3	22.59368896484375	24.773406982421875	30.501556396484375	22.13134765625
4	14.815521240234375	18.684005737304688	33.61854553222656	32.881927490234375
5	14.0411376953125	38.33732604980469	32.32994079589844	15.291595458984375
6	30.120468139648438	34.52415466308594	17.389678955078125	17.9656982421875
7	25.45013427734375	33.34236145019531	21.318435668945312	19.889068603515625
8	28.377914428710938	32.42340087890625	20.389556884765625	18.809127807617188
9	26.605987548828125	13.58184814453125	21.8505859375	37.961578369140625
10	21.618270874023438	27.596664428710938	28.873443603515625	21.91162109375
11	32.96699523925781	24.09515380859375	19.638442993164062	23.299407958984375
12	20.151138305664062	28.721237182617188	28.680419921875	22.44720458984375
13	32.06024169921875	20.0836181640625	26.27716064453125	21.5789794921875
14	22.057342529296875	24.065017700195312	28.050994873046875	25.826644897460938
15	28.980636596679688	26.197052001953125	24.106597900390625	20.715713500976562
16	21.588897705078125	26.404953002929688	28.46221923828125	23.543930053710938
17	20.882034301757812	28.604888916015625	27.3345947265625	23.178482055664062
18	20.136642456054688	25.132369995117188	30.52215576171875	24.208831787109375
19	20.856094360351562	27.502059936523438	31.233978271484375	20.407867431640625
20	21.9970703125	24.543380737304688	32.958221435546875	20.501327514648438
21	23.084259033203125	25.507354736328125	30.633163452148438	20.775222778320312
22	22.874069213867188	26.265716552734375	29.94842529296875	20.911788940429688
23	22.614288330078125	26.273727416992188	30.185317993164062	20.926666259765625
24	21.48895263671875	26.6632080078125	30.591583251953125	21.256256103515625
25	21.9024658203125	27.384185791015625	30.637359619140625	20.07598876953125
26	22.349929809570312	26.906967163085938	30.267333984375	20.47576904296875
27	21.33026123046875	26.994705200195312	31.124114990234375	20.550918579101562
28	20.108795166015625	28.891372680664062	30.248641967773438	20.751190185546875
29	20.900344848632812	28.097152709960938	29.673004150390625	21.329498291015625
30	20.204544067382812	29.604339599609375	31.317901611328125	18.873214721679688
31	21.561431884765625	27.237319946289062	30.121231079101562	21.08001708984375
32	19.378280639648438	28.586196899414062	29.9652099609375	22.0703125
33	20.496749877929688	27.974700927734375	30.768203735351562	20.760345458984375
34	18.95294189453125	28.569412231445312	30.47027587890625	22.007369995117188
35	20.626068115234375	28.94439697265625	30.166244506835938	20.263290405273438
36	20.127487182617188	28.8909912109375	29.875564575195312	21.10595703125
37	20.479583740234375	29.340744018554688	29.40521240234375	20.774459838867188
38	19.858551025390625	28.377151489257812	29.795455932617188	21.968841552734375
39	20.368194580078125	27.267837524414062	31.380844116210938	20.983123779296875
40	19.829177856445312	28.484344482421875	30.741119384765625	20.945358276367188
41	19.635772705078125	27.085113525390625	31.211471557617188	22.067642211914062
42	19.074249267578125	27.825164794921875	30.916213989257812	22.184371948242188
43	19.49005126953125	28.028488159179688	30.282211303710938	22.199249267578125
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	56.0
1	65.0
2	74.0
3	221.5
4	369.0
5	369.0
6	545.5
7	722.0
8	757.0
9	792.0
10	1186.5
11	1581.0
12	1581.0
13	2963.0
14	4345.0
15	6584.0
16	8823.0
17	7719.5
18	6616.0
19	6616.0
20	8208.5
21	9801.0
22	7100.0
23	4399.0
24	4365.5
25	4332.0
26	4332.0
27	4658.0
28	4984.0
29	4962.5
30	4941.0
31	5255.5
32	5570.0
33	5570.0
34	6338.5
35	7107.0
36	7423.5
37	7740.0
38	8752.0
39	9764.0
40	9764.0
41	10811.5
42	11859.0
43	14086.0
44	16313.0
45	18782.5
46	21252.0
47	21252.0
48	24313.5
49	27375.0
50	24470.5
51	21566.0
52	20387.0
53	19208.0
54	19208.0
55	18586.0
56	17964.0
57	16516.5
58	15069.0
59	12490.5
60	9912.0
61	9912.0
62	8273.5
63	6635.0
64	5459.0
65	4283.0
66	3730.5
67	3178.0
68	3178.0
69	2835.5
70	2493.0
71	1959.0
72	1425.0
73	1111.5
74	798.0
75	798.0
76	617.0
77	436.0
78	316.0
79	196.0
80	149.5
81	103.0
82	103.0
83	75.5
84	48.0
85	30.0
86	12.0
87	7.5
88	3.0
89	3.0
90	1.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	262144.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.823898315429688
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.92434686830934	21.861648559570312
2	11.951288937291931	7.367706298828125
3	4.93917305050555	4.5673370361328125
4	2.6818311201316782	3.30657958984375
5	1.660829424650075	2.559661865234375
6	1.17693649988243	2.176666259765625
7	0.8366026013885624	1.80511474609375
8	0.6447780404192913	1.5899658203125
9	0.5111196366471542	1.4179229736328125
>10	4.00851453535141	24.639892578125
>50	0.4195388785069861	8.875274658203125
>100	0.23018947316312513	12.985610961914062
>500	0.009900622501639792	1.66778564453125
>1k	0.0037127334381149213	3.12042236328125
>5k	0.001237577812704974	2.05841064453125
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5396	2.05841064453125	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3965	1.5125274658203125	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2754	1.050567626953125	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1461	0.5573272705078125	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	641	0.2445220947265625	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	569	0.2170562744140625	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	561	0.2140045166015625	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	542	0.206756591796875	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	529	0.2017974853515625	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	513	0.1956939697265625	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	513	0.1956939697265625	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	504	0.1922607421875	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	482	0.183868408203125	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	466	0.177764892578125	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	448	0.1708984375	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	442	0.168609619140625	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	436	0.16632080078125	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	434	0.165557861328125	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	397	0.1514434814453125	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	389	0.1483917236328125	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	388	0.14801025390625	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	374	0.142669677734375	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	372	0.14190673828125	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	346	0.131988525390625	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	342	0.130462646484375	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	337	0.1285552978515625	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	334	0.127410888671875	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	324	0.12359619140625	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	322	0.122833251953125	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	315	0.1201629638671875	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	310	0.118255615234375	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	308	0.11749267578125	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	300	0.11444091796875	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	284	0.10833740234375	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	283	0.1079559326171875	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	276	0.10528564453125	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	274	0.104522705078125	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	272	0.103759765625	No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	269	0.1026153564453125	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	265	0.1010894775390625	No Hit
CTGTCACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAAC	264	0.1007080078125	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	3.814697265625E-4	0.0
17	0.0	0.0	0.0	0.0011444091796875	0.0
18	0.0	0.0	0.0	0.0011444091796875	0.0
19	0.0	0.0	0.0	0.0026702880859375	0.0
20	0.0	0.0	0.0	0.0041961669921875	0.0
21	0.0	0.0	0.0	0.0049591064453125	0.0
22	0.0	0.0	0.0	0.0091552734375	0.0
23	0.0	0.0	0.0	0.014495849609375	0.0
24	0.0	0.0	0.0	0.0278472900390625	0.0
25	0.0	0.0	0.0	0.031280517578125	0.0
26	0.0	0.0	0.0	0.0408172607421875	0.0
27	0.0	0.0	0.0	0.060272216796875	0.0
28	0.0	0.0	0.0	0.0888824462890625	0.0
29	0.0	0.0	0.0	0.1312255859375	0.0
30	0.0	0.0	0.0	0.202178955078125	0.0
31	0.0	0.0	0.0	0.3826141357421875	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATAATCC	25	0.0054887016	29.599998	3
CATCTGT	25	0.0054887016	29.599998	37
GTAATCT	25	0.0054887016	29.599998	36
TTGGATA	40	5.9229154E-5	27.75	14
AATCCTC	35	8.8510616E-4	26.428572	5
TAATCCT	35	8.8510616E-4	26.428572	4
CTAGATG	45	1.3194082E-4	24.666668	3
CTATCTT	45	1.3194082E-4	24.666668	9
CGCACTA	45	1.3194082E-4	24.666668	29
ATACCAT	55	1.895403E-5	23.545454	6
ACCATGC	40	0.0019272338	23.125	8
AACCAGA	40	0.0019272338	23.125	1
CAGGCAG	40	0.0019272338	23.125	10
GTATACT	40	0.0019272338	23.125	4
TCTTGGA	50	2.694398E-4	22.199999	12
ACACGTT	50	2.694398E-4	22.199999	14
ACGTTTC	50	2.694398E-4	22.199999	16
ATATGGC	50	2.694398E-4	22.199999	23
TATCTTG	50	2.694398E-4	22.199999	10
TGAATGG	50	2.694398E-4	22.199999	29
>>END_MODULE