FastQCFastQC Report
Fri 10 Feb 2017
ERR1630245.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630245.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228304
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT59182.5921578246548465No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT42321.85366879248721No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT35371.549250122643493No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18410.8063809657299039No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA9400.4117317261195599No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA6060.2654355596047375No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT5790.25360922279066506No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA5740.2514191604176887No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA5500.24090686102740205No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA5410.2369647487560446No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA5340.23389866143387764No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5030.22032027472142407No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT4810.21068400028032797No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA4780.20936996285654216No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG4770.2089319503819469No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA4340.19009741397434998No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC3850.16863480271918144No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA3830.1677587777699909No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3810.16688275282080034No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA3790.16600672787160978No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3780.1655687153970145No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC3770.16513070292241924No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC3730.16337865302403812No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC3720.16294064054944285No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA3530.15461840353213258No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA3530.15461840353213258No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC3410.14936225383698928No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG3390.14848622888779872No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC3330.14585815404022706No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA3090.13534585464994042No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC2960.12965169248020184No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG2920.1278996425818207No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG2840.12439554278505852No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2800.1226434928866774No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT2780.12176746793748686No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA2630.1151972808185577No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA2620.11475926834396244No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG2590.11344523092017662No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA2550.11169318102179551No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2540.11125516854720023No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT2520.11037914359800967No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC2500.10950311864881912No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA2490.10906510617422385No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG2480.10862709369962857No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2470.10818908122503328No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT2460.10775106875043801No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT2390.10468498142827108No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.10337094400448524No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA2350.10293293152988997No Hit
CCCATATTCAGACAGGATACCACGTGTCCCGCCCTACTCATCG2300.10074286915691358No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGATCAC303.5905276E-430.83333421
AGTCCTA601.2587407E-930.83333429
GAGTCCT601.2587407E-930.83333428
TATTGAC303.5905276E-430.83333430
TCATCCA601.2587407E-930.83333414
CAATGCG601.2587407E-930.83333419
TAGTACT551.8420906E-830.2727284
TTAGGTC502.7046553E-729.620
CGCGCTT250.00548723629.617
GGCCACC250.00548723629.611
AGACGGC250.00548723629.634
AGGCCAC250.00548723629.610
GTCGTCA502.7046553E-729.624
CCGCGCT250.00548723629.616
GCGCTTA250.00548723629.618
CGTCATT502.7046553E-729.626
CATCCAA652.9631337E-928.46153815
CTAGTAC754.5838533E-1027.1333333
ATTAGGT556.224873E-726.9090919
CTACACT556.224873E-726.9090933
Produced by FastQC (version 0.11.5)