FastQCFastQC Report
Fri 10 Feb 2017
ERR1630244.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630244.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences213983
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35731.6697588126159555No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT30531.4267488538809157No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT21821.0197071729997242No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10820.505647644906371No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4700.2196436165489782No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT3530.16496637583359428No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3250.1518812242093998No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3160.14767528261590873No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3010.1406653799600903No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2960.13832874574148415No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2690.1257109209610109No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2620.1224396330549623No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2600.12150497936751985No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA2480.1158970572428651No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2440.11402774986798017No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2420.11309309618053771No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2230.10421388614983433No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAAG250.00548647629.59999835
AATTCTA250.00548647629.59999823
TTGTTCA250.00548647629.5999982
ACCATAG358.8457234E-426.42857232
TGATTGT451.3183011E-424.66666618
CTACTAC400.001926081623.1250021
TGGACAC400.001926081623.1250025
AGTACTG855.1311872E-821.7647065
GCAGTCG1850.021.09
GTCCTAA450.003815791620.5555551
TCCTACT450.003815791620.5555552
TCCTAAG450.003815791620.5555552
CATAGGA450.003815791620.55555534
GATTGTT450.003815791620.55555519
CTACACT450.003815791620.5555554
AATAGCA450.003815791620.5555555
TGGTGAT450.003815791620.55555515
CGGTGAT1850.020.014
GGGCAGT2050.019.8536577
CAGTCGG1900.019.47368410
Produced by FastQC (version 0.11.5)