##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630244.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	213983
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.80238617086404	31.0	31.0	34.0	30.0	34.0
2	31.953739315740034	33.0	31.0	34.0	30.0	34.0
3	32.01545449872186	33.0	31.0	34.0	30.0	34.0
4	35.72302939953174	37.0	35.0	37.0	33.0	37.0
5	35.68349822182136	37.0	35.0	37.0	33.0	37.0
6	35.76500936990322	37.0	35.0	37.0	35.0	37.0
7	35.75390101082796	37.0	35.0	37.0	35.0	37.0
8	35.764219587537326	37.0	35.0	37.0	35.0	37.0
9	37.419061327301705	39.0	37.0	39.0	34.0	39.0
10	37.35421505446694	39.0	37.0	39.0	34.0	39.0
11	37.44374085791862	39.0	37.0	39.0	35.0	39.0
12	37.33305916825169	39.0	37.0	39.0	34.0	39.0
13	37.384287536860406	39.0	37.0	39.0	34.0	39.0
14	38.44464747199544	40.0	38.0	41.0	34.0	41.0
15	38.44392311538767	40.0	38.0	41.0	34.0	41.0
16	38.33648000074772	40.0	38.0	41.0	34.0	41.0
17	38.41938845609231	40.0	38.0	41.0	34.0	41.0
18	38.477692153114965	40.0	38.0	41.0	34.0	41.0
19	38.51587275624699	40.0	38.0	41.0	34.0	41.0
20	38.51299869615811	40.0	38.0	41.0	34.0	41.0
21	38.47466387516765	40.0	38.0	41.0	34.0	41.0
22	38.440039629316345	40.0	38.0	41.0	34.0	41.0
23	38.386329755167466	40.0	38.0	41.0	34.0	41.0
24	38.34032142740311	40.0	38.0	41.0	34.0	41.0
25	38.297822724235104	40.0	38.0	41.0	34.0	41.0
26	38.083291663356434	40.0	37.0	41.0	33.0	41.0
27	37.95551515774618	40.0	37.0	41.0	33.0	41.0
28	37.84064154629106	40.0	37.0	41.0	33.0	41.0
29	37.7323432235271	40.0	37.0	41.0	33.0	41.0
30	37.568147936985646	39.0	36.0	41.0	33.0	41.0
31	37.34451334919129	39.0	36.0	41.0	32.0	41.0
32	37.195758541566384	39.0	35.0	41.0	32.0	41.0
33	37.09704509236715	39.0	35.0	41.0	32.0	41.0
34	36.96103896103896	39.0	35.0	41.0	31.0	41.0
35	36.830173424991706	39.0	35.0	41.0	31.0	41.0
36	36.63935452816345	39.0	35.0	41.0	31.0	41.0
37	36.513251052653715	39.0	35.0	40.0	30.0	41.0
38	36.32707738465205	39.0	35.0	40.0	30.0	41.0
39	36.15322245225088	39.0	35.0	40.0	30.0	41.0
40	35.94605646242926	38.0	35.0	40.0	29.0	41.0
41	35.700532285274996	38.0	35.0	40.0	28.0	41.0
42	35.51121818088353	38.0	35.0	40.0	27.0	41.0
43	34.28987349462341	37.0	33.0	40.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	1.0
11	1.0
12	2.0
13	4.0
14	0.0
15	1.0
16	2.0
17	6.0
18	9.0
19	13.0
20	48.0
21	73.0
22	144.0
23	230.0
24	426.0
25	613.0
26	1101.0
27	1557.0
28	2009.0
29	2954.0
30	3806.0
31	5000.0
32	6378.0
33	8238.0
34	10988.0
35	14423.0
36	20434.0
37	32584.0
38	47778.0
39	55159.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.211923377090706	18.74307772112738	14.478252945327434	22.566745956454483
2	19.254800615002125	22.668623208385714	34.00643976390648	24.070136412705683
3	21.05354163648514	23.914983900590233	31.752055069795265	23.27941939312936
4	14.67172625862802	17.331283326245543	32.68811073776889	35.308879677357545
5	13.88147656589542	38.396508133823716	32.7212909436731	15.000724356607767
6	31.7506530892641	34.002701149156714	16.716281199908405	17.530364561670787
7	26.69464396704411	32.84746919147783	20.176369150820392	20.28151769065767
8	29.281765373884845	32.267049251576054	19.683806657538216	18.76737871700088
9	27.075048017833193	13.14730609440937	20.13103844697943	39.646607440778006
10	20.46424248655267	27.610604580737725	28.24009383923022	23.68505909347939
11	33.9475565815976	23.62056798904586	18.662697504007326	23.76917792534921
12	21.01148222055023	29.06118710364842	27.398438193688285	22.528892482113065
13	32.93158802334764	18.882808447400027	25.46697634858844	22.718627180663884
14	23.106508460952504	22.574690512797744	26.439950837216042	27.87885018903371
15	28.73172167882495	26.113756700298623	23.402326353028045	21.752195267848382
16	22.645724193043375	25.933368538622226	26.541360762303544	24.879546506030852
17	21.191403055382903	28.159713622110168	26.201146820074495	24.447736502432434
18	21.004939644738133	23.72337989466453	29.601884261833884	25.66979619876345
19	22.000345821864354	26.732497441385533	29.858914025880562	21.408242710869555
20	23.441114481056907	23.3925124893099	31.52586887743419	21.640504152199007
21	25.359491174532558	24.17201366463691	28.714897912450986	21.753597248379545
22	25.008528714897913	24.520172163209228	27.54050555417954	22.930793567713323
23	23.657019482856114	25.437534757434	28.23355126341812	22.67189449629176
24	22.82284106681372	26.320782492067124	29.04576531780562	21.810611123313535
25	23.008369823771048	25.899721005874298	28.927531626344148	22.164377544010506
26	24.09163344751686	25.597360537986663	28.314866134225614	21.996139880270864
27	22.97939555946033	25.694097194636957	28.62937710005	22.69713014585271
28	21.64237345957389	26.691372679138066	29.490193146184513	22.176060715103535
29	22.310183519251527	26.735768729291582	29.09857325114612	21.855474500310773
30	21.4708645079282	28.476561222153162	29.69534963057813	20.357224639340508
31	22.742460849693664	26.357701312721105	28.69293355079609	22.206904286789136
32	20.367038503058655	26.964291555871263	29.299056467102524	23.369613473967558
33	21.13251987307403	26.585289485613345	30.299603239509683	21.98258740180295
34	20.928298042367853	26.87643410925167	29.022866302463278	23.172401545917197
35	21.437684302023992	27.283943116976584	29.447199076562157	21.831173504437267
36	20.30535135968745	27.619016463924705	29.15138118448662	22.924250991901225
37	21.298888229438788	27.3283391671301	29.13736137917498	22.235411224256133
38	20.68762471785142	26.12497254454793	29.52757929368221	23.659823443918444
39	20.663323721977914	25.36509909665721	31.137520270301845	22.834056911063026
40	20.079165167326376	25.804853656598887	31.44361935293925	22.672361823135486
41	19.319759046279376	25.870746741563583	31.76654220195062	23.04295201020642
42	19.504820476392982	25.78569325600632	31.690367926424063	23.019118341176636
43	19.02066986629779	26.853067767065607	31.31650645144708	22.809755915189527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	25.0
2	43.0
3	146.5
4	250.0
5	250.0
6	382.5
7	515.0
8	487.0
9	459.0
10	636.5
11	814.0
12	814.0
13	1730.5
14	2647.0
15	4628.5
16	6610.0
17	5562.5
18	4515.0
19	4515.0
20	5099.0
21	5683.0
22	4010.0
23	2337.0
24	2469.5
25	2602.0
26	2602.0
27	2681.5
28	2761.0
29	3043.0
30	3325.0
31	3546.0
32	3767.0
33	3767.0
34	4301.0
35	4835.0
36	5093.0
37	5351.0
38	6473.5
39	7596.0
40	7596.0
41	9014.5
42	10433.0
43	11622.0
44	12811.0
45	15592.0
46	18373.0
47	18373.0
48	21030.0
49	23687.0
50	21730.5
51	19774.0
52	19003.0
53	18232.0
54	18232.0
55	16566.5
56	14901.0
57	13757.5
58	12614.0
59	10834.5
60	9055.0
61	9055.0
62	7747.5
63	6440.0
64	5423.0
65	4406.0
66	3819.0
67	3232.0
68	3232.0
69	2808.5
70	2385.0
71	1953.5
72	1522.0
73	1181.0
74	840.0
75	840.0
76	681.0
77	522.0
78	416.5
79	311.0
80	238.5
81	166.0
82	166.0
83	121.0
84	76.0
85	61.0
86	46.0
87	32.5
88	19.0
89	19.0
90	16.5
91	14.0
92	9.5
93	5.0
94	3.0
95	1.0
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	213983.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.757840576120536
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.33259799415814	37.21604052658389
2	7.829319607002063	7.165055167933901
3	3.219151500296178	4.419042634227953
4	1.6739179279776129	3.063794787436385
5	1.100966153971853	2.518891687657431
6	0.7598504810343771	2.0861470303715715
7	0.5515043813959188	1.76649546926625
8	0.44120350511673506	1.6150815719005716
9	0.3492861082174153	1.4384320249739466
>10	2.465428845721756	21.856409153998214
>50	0.18894131584860183	5.931312300509854
>100	0.08374696161938028	6.301435160737068
>500	0.0	0.0
>1k	0.004085217639969769	4.621862484402967
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3573	1.6697588126159555	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3053	1.4267488538809157	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2182	1.0197071729997242	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1082	0.505647644906371	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	470	0.2196436165489782	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	353	0.16496637583359428	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	325	0.1518812242093998	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	316	0.14767528261590873	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	301	0.1406653799600903	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	296	0.13832874574148415	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	269	0.1257109209610109	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	262	0.1224396330549623	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	260	0.12150497936751985	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	248	0.1158970572428651	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	244	0.11402774986798017	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	242	0.11309309618053771	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	223	0.10421388614983433	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	4.673268437212302E-4	0.0
13	0.0	0.0	0.0	0.0014019805311636907	0.0
14	0.0	0.0	0.0	0.0014019805311636907	0.0
15	0.0	0.0	0.0	0.0018693073748849208	0.0
16	0.0	0.0	0.0	0.005607922124654763	0.0
17	0.0	0.0	0.0	0.010748517405588295	0.0
18	0.0	0.0	0.0	0.013552478467915675	0.0
19	0.0	0.0	0.0	0.014954458999079366	0.0
20	0.0	0.0	0.0	0.016356439530243058	0.0
21	0.0	0.0	0.0	0.02383366902978274	0.0
22	0.0	0.0	0.0	0.032712879060486116	0.0
23	0.0	0.0	0.0	0.043928723309795636	0.0
24	0.0	0.0	0.0	0.06776239233957838	0.0
25	0.0	0.0	0.0	0.08131487080749406	0.0
26	0.0	0.0	0.0	0.09112873452563988	0.0
27	0.0	0.0	0.0	0.10748517405588294	0.0
28	0.0	0.0	0.0	0.13973072627264782	0.0
29	0.0	0.0	0.0	0.19394064014431053	0.0
30	0.0	0.0	0.0	0.3402139422290556	0.0
31	0.0	0.0	0.0	0.7636120626404901	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGAAG	25	0.005486476	29.599998	35
AATTCTA	25	0.005486476	29.599998	23
TTGTTCA	25	0.005486476	29.599998	2
ACCATAG	35	8.8457234E-4	26.428572	32
TGATTGT	45	1.3183011E-4	24.666666	18
CTACTAC	40	0.0019260816	23.125002	1
TGGACAC	40	0.0019260816	23.125002	5
AGTACTG	85	5.1311872E-8	21.764706	5
GCAGTCG	185	0.0	21.0	9
GTCCTAA	45	0.0038157916	20.555555	1
TCCTACT	45	0.0038157916	20.555555	2
TCCTAAG	45	0.0038157916	20.555555	2
CATAGGA	45	0.0038157916	20.555555	34
GATTGTT	45	0.0038157916	20.555555	19
CTACACT	45	0.0038157916	20.555555	4
AATAGCA	45	0.0038157916	20.555555	5
TGGTGAT	45	0.0038157916	20.555555	15
CGGTGAT	185	0.0	20.0	14
GGGCAGT	205	0.0	19.853657	7
CAGTCGG	190	0.0	19.473684	10
>>END_MODULE