FastQCFastQC Report
Fri 10 Feb 2017
ERR1630243.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630243.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences349919
Sequences flagged as poor quality0
Sequence length43
%GC39

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT156674.477321894495583No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT112303.2093141555617155No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT86392.4688570783524186No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT42081.2025640219593676No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA25680.7338841274695No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA15250.435815145790883No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT14590.4169536378419006No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13990.3998068124337346No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT11420.3263612436020908No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA9010.25748816154595777No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC8210.23462572766840326No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA8180.23376838639799496No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA8140.23262526470411724No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA7700.22005092607146223No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA7590.21690734141329848No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.21290641548472647No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7340.20976283082656272No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA7190.20547612447452124No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC7100.2029041006632964No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA6930.19804583346431603No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA6930.19804583346431603No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG6770.19347334668880514No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA6630.1894724207602331No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6590.18832929906635534No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA6530.18661461652553876No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT6410.1831852514439056No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA6410.1831852514439056No Hit
GTACATGGGAAGCAGTGGTATCAAAAAAAAAAAAAAAAAAAAA6160.1760407408571698No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG5990.17118247365818948No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC5740.16403796307145369No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC5510.15746501333165674No Hit
GTACATGGGAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAA5290.15117784401532927No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT5280.15089206359185983No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG5020.14346177258165463No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG4760.1360314815714494No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG4670.1334594577602245No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA4610.13174477521940792No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC4510.1288869709847136No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA4510.1288869709847136No Hit
GAAGAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4500.12860119056124417No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA4420.1263149471734887No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC4320.12345714293879441No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT4250.12145667997450837No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4170.11917043658675294No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC4130.11802731489287521No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA4050.11574107150511975No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG4040.11545529108165033No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT4000.11431216938777261No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA3990.11402638896430316No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT3990.11402638896430316No Hit
GAAGCATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA3990.11402638896430316No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC3950.11288326727042544No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA3890.11116858472960886No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT3880.11088280430613942No Hit
CTCCAAGGCTAAATACTCCTGACTGACCGATAGTGAACCAGTA3880.11088280430613942No Hit
CACACACACTGATTCAGGCTCTGGGCTGCTCCCCGTTCGCTCG3850.11002546303573112No Hit
CCGCTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTA3810.1088823413418534No Hit
CACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCG3770.10773921964797567No Hit
CATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAAA3620.1034525132959342No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTGT3560.10173783075511761No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTACGA200.001840187137.010
TGGCCCG200.001840187137.05
CGAGGCT200.001840187137.014
TTTTACG352.3835973E-531.7142854
CTTTTGC303.5944083E-430.83333224
CTACCTA303.5944083E-430.83333214
TACCCGG250.00549118529.59999820
CCCACTC250.00549118529.59999833
CTACCCG250.00549118529.59999819
GCTACCC250.00549118529.59999818
TAAGCTA250.00549118529.59999810
TAGTAGT250.00549118529.5999984
AGGCTAC250.00549118529.59999816
CAGTCGG851.8189894E-1228.29411710
AGTCGGT851.8189894E-1228.29411711
TTTACGA405.9285074E-527.755
CGTATCC358.8570203E-426.428573
TACCTAC358.8570203E-426.4285715
TTGATCA451.3206441E-424.66666620
CCCTCGG451.3206441E-424.66666622
Produced by FastQC (version 0.11.5)