FastQCFastQC Report
Fri 10 Feb 2017
ERR1630242.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630242.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1010939
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC60100.5944967995101583No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT35810.3542251312888315No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG29720.2939841078442913No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG23520.23265498709615515No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT22750.22503830597098343No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG21260.21029953340409263No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT20180.19961639624151406No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG18910.18705381828181522No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT18890.18685598240843415No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT17970.17775553223290427No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG17910.177162024612761No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC17250.17063344079118523No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG16190.16014813950198775No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG16190.16014813950198775No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG16060.1588622063250107No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG15510.1534217198070309No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT15370.1520368686933633No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC15180.150157427896243No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT13710.1356164912027333No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT13330.13185760960849272No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC13020.1287911535710859No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA12960.12819764595094263No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT11680.11553615005455324No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG11660.11533831418117217No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA11600.11474480656102891No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA11410.11286536576390861No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA11260.11138159671355047No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT11190.11068917115671667No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC11140.11019458147326397No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC10270.1015887209811868No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT10130.10020386986751921No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGCGGG451.3230582E-424.66666628
ACAATCG400.001931026923.12500237
GGTATCA5300.022.6886791
GGCGTAA555.142505E-420.18181813
TGCATCA12000.019.73333414
CAGGACA13050.019.704984
GACAGGC12700.019.5196867
GCATCAG12150.019.48971215
CCAGGAC14350.018.693383
GCTGCAT12800.018.64453112
TTTAGCG701.2190125E-418.526
TAGAGTA500.00703430918.55
GTATTAT801.6163563E-518.51
ACAGGCT13500.018.58
TATAGAC801.6163563E-518.53
GCGTAAA609.235767E-418.514
GGACAGG14100.018.2375896
TCCAGGA14850.017.6902352
CTGCATC13500.017.54074113
GCCGATC852.72259E-517.41176424
Produced by FastQC (version 0.11.5)