##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630242.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1010939
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.90133331486865	33.0	31.0	34.0	30.0	34.0
2	32.128986021906364	33.0	31.0	34.0	30.0	34.0
3	32.24468538655646	34.0	31.0	34.0	30.0	34.0
4	35.893458457928716	37.0	35.0	37.0	35.0	37.0
5	35.8312232488805	37.0	35.0	37.0	35.0	37.0
6	35.87691838973469	37.0	35.0	37.0	35.0	37.0
7	35.85697059862168	37.0	35.0	37.0	35.0	37.0
8	35.844582116230555	37.0	35.0	37.0	35.0	37.0
9	37.62030943508955	39.0	37.0	39.0	35.0	39.0
10	37.51181129623053	39.0	37.0	39.0	35.0	39.0
11	37.581204207177684	39.0	37.0	39.0	35.0	39.0
12	37.50138633488272	39.0	37.0	39.0	35.0	39.0
13	37.495052619396425	39.0	37.0	39.0	35.0	39.0
14	38.76449123043032	40.0	38.0	41.0	35.0	41.0
15	38.77713393191874	40.0	38.0	41.0	35.0	41.0
16	38.74439901912974	40.0	38.0	41.0	35.0	41.0
17	38.697120202109126	40.0	38.0	41.0	35.0	41.0
18	38.58461193009667	40.0	38.0	41.0	34.0	41.0
19	38.609447256461564	40.0	38.0	41.0	34.0	41.0
20	38.595354418021266	40.0	38.0	41.0	34.0	41.0
21	38.551073803661744	40.0	38.0	41.0	34.0	41.0
22	38.5025407072039	40.0	38.0	41.0	34.0	41.0
23	38.4564271434775	40.0	38.0	41.0	34.0	41.0
24	38.41770670633935	40.0	38.0	41.0	34.0	41.0
25	38.4031400509823	40.0	38.0	41.0	34.0	41.0
26	38.226054193180794	40.0	38.0	41.0	34.0	41.0
27	38.12010516955029	40.0	38.0	41.0	33.0	41.0
28	38.04271771095981	40.0	38.0	41.0	33.0	41.0
29	37.97519830573358	40.0	38.0	41.0	33.0	41.0
30	37.9218271329922	40.0	37.0	41.0	33.0	41.0
31	37.91583567356685	40.0	37.0	41.0	33.0	41.0
32	37.844915469677204	40.0	37.0	41.0	33.0	41.0
33	37.755678631450564	40.0	37.0	41.0	33.0	41.0
34	37.68138334756103	40.0	37.0	41.0	33.0	41.0
35	37.53549818535045	40.0	37.0	41.0	32.0	41.0
36	37.477470945329046	40.0	37.0	41.0	32.0	41.0
37	37.40855877555421	40.0	37.0	41.0	32.0	41.0
38	37.32472879174708	40.0	36.0	41.0	31.0	41.0
39	37.26762841279246	39.0	36.0	41.0	31.0	41.0
40	37.14033289842414	39.0	36.0	41.0	31.0	41.0
41	37.02007539525135	39.0	36.0	41.0	31.0	41.0
42	37.02380756900268	39.0	36.0	41.0	31.0	41.0
43	35.71389272745438	38.0	34.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	1.0
10	2.0
11	4.0
12	1.0
13	2.0
14	3.0
15	1.0
16	5.0
17	11.0
18	37.0
19	70.0
20	130.0
21	306.0
22	534.0
23	896.0
24	1507.0
25	2424.0
26	3435.0
27	5077.0
28	7181.0
29	10224.0
30	13914.0
31	18576.0
32	24521.0
33	32579.0
34	45959.0
35	61477.0
36	84610.0
37	128416.0
38	243916.0
39	325119.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.4961951215652	16.658769718054206	11.210369765139143	28.634665395241456
2	19.721071202120008	18.781054049749788	33.37332915240188	28.12454559572833
3	20.570479524481694	20.59827546469174	28.614782889966655	30.216462120859916
4	15.90066265125789	14.157332935023776	31.91221230954588	38.02979210417246
5	16.36221374385596	34.99667141143036	32.543407663568225	16.09770718114545
6	38.332975580128966	31.960583180587555	14.720373830666341	14.986067408617137
7	32.614331824175345	28.349682819636	19.369714690995206	19.66627066519345
8	29.7114860540547	32.092341872259354	18.45699888915157	19.73917318453438
9	28.539902011891915	13.15499748253851	16.842460326488542	41.462640179081035
10	18.277363916121548	25.451782946349876	31.122154749198515	25.14869838833006
11	38.742693673901194	19.975092463541323	19.559637129441043	21.72257673311644
12	24.957094344960478	22.495521490416337	27.38414483959962	25.163239325023568
13	31.999260093833552	17.42380104041886	22.97636158066906	27.600577285078526
14	24.1023444540175	17.906025981785252	23.48677813399226	34.50485143020499
15	27.50067016902108	24.70950274942405	20.27609974489064	27.51372733666423
16	26.97561376106768	23.83378225590268	20.686015674536247	28.50458830849339
17	25.692351368381278	24.629675974514782	23.420700952282978	26.257271704820962
18	26.02431996391474	22.57099587611122	24.10086068496714	27.3038234750069
19	27.136849997873263	22.66358306485357	23.101492770582595	27.098074166690573
20	26.50634706940775	23.717949352038055	23.145115580663127	26.63058799789107
21	29.184352369430794	22.58603140248818	21.987676803447094	26.24193942463393
22	28.479660988447375	23.31307823716367	22.091936308718925	26.115324465670035
23	27.5506237270498	23.44899148217647	21.851565722560906	27.14881906821282
24	27.778233899374737	22.788219665083652	22.619564583026275	26.813981852515333
25	27.23833980091776	22.498785782327126	23.306945325088854	26.955929091666263
26	28.211395544142626	22.61343167095146	23.245616204340717	25.9295565805652
27	28.466900574614296	22.460603458764574	22.085506642834037	26.986989323787093
28	26.873827204213114	21.87204173545585	23.752966301626508	27.501164758704533
29	26.89885344219582	22.070174362647005	24.02538629927226	27.005585895884916
30	26.348869714196404	22.872497747143992	23.613689846766224	27.16494269189338
31	26.501895762256673	23.045900890162514	23.256101505629914	27.1961018419509
32	26.063590384780884	23.087941013255993	23.67135900385681	27.177109598106313
33	27.666357712977735	22.98872632275538	22.95222560411657	26.39269036015032
34	27.59543355237062	23.174692043733597	23.0816102653078	26.148264138587983
35	27.572583508995102	22.681487211394554	23.868007861997608	25.877921417612736
36	27.26395954652061	23.441572636924683	21.980950383752134	27.313517432802577
37	25.943207255828494	22.99812352674098	23.596082454035308	27.46258676339522
38	27.22360102835087	22.726494872588752	23.72803898158049	26.321865117479888
39	25.448815408249164	22.788912090640483	24.46685705072215	27.295415450388205
40	26.320974856049673	21.330762785885202	25.768320343759616	26.579942014305512
41	24.874300031950494	21.6570930590273	26.144406339057053	27.32420056996515
42	24.812476321518908	22.296597519731655	25.17530731329981	27.715618845449626
43	24.905063510261254	19.829782014542914	27.11192267782725	28.153231797368583
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	19.0
1	16.0
2	13.0
3	41.5
4	70.0
5	70.0
6	93.0
7	116.0
8	120.5
9	125.0
10	154.0
11	183.0
12	183.0
13	391.5
14	600.0
15	975.0
16	1350.0
17	1406.0
18	1462.0
19	1462.0
20	1825.0
21	2188.0
22	2580.5
23	2973.0
24	3830.0
25	4687.0
26	4687.0
27	6097.5
28	7508.0
29	9672.5
30	11837.0
31	14390.0
32	16943.0
33	16943.0
34	20549.0
35	24155.0
36	27935.0
37	31715.0
38	35249.0
39	38783.0
40	38783.0
41	42349.5
42	45916.0
43	49021.5
44	52127.0
45	54205.0
46	56283.0
47	56283.0
48	58432.5
49	60582.0
50	65695.5
51	70809.0
52	77658.0
53	84507.0
54	84507.0
55	87496.0
56	90485.0
57	86665.5
58	82846.0
59	74626.5
60	66407.0
61	66407.0
62	65033.0
63	63659.0
64	55992.5
65	48326.0
66	40737.0
67	33148.0
68	33148.0
69	28190.5
70	23233.0
71	21676.0
72	20119.0
73	23572.5
74	27026.0
75	27026.0
76	25802.5
77	24579.0
78	18314.5
79	12050.0
80	7335.5
81	2621.0
82	2621.0
83	1734.5
84	848.0
85	607.5
86	367.0
87	262.0
88	157.0
89	157.0
90	118.5
91	80.0
92	54.0
93	28.0
94	17.5
95	7.0
96	7.0
97	4.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1010939.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	55.33550763224668
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.85998442279123	46.40434808067451
2	9.803285213787268	10.849395275372329
3	2.616195435235125	4.343055074217066
4	1.1198482825384726	2.4786949274146393
5	0.5840947286544632	1.6160589157707053
6	0.37414248793604976	1.2422018698039825
7	0.2570763203320782	0.9957814080063925
8	0.19279378138162526	0.8534673408874097
9	0.14521881209307866	0.7232181016430117
>10	0.8882873404469033	9.306787263371907
>50	0.07864489163116867	3.0059913136752994
>100	0.062157270254033094	7.71955555218638
>500	0.012718058012035393	4.988668579459761
>1k	0.005373827329029039	4.877058159612919
>5k	1.791275776343013E-4	0.5957181379036571
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	6010	0.5944967995101583	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	3581	0.3542251312888315	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	2972	0.2939841078442913	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	2352	0.23265498709615515	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	2275	0.22503830597098343	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2126	0.21029953340409263	No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT	2018	0.19961639624151406	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	1891	0.18705381828181522	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	1889	0.18685598240843415	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	1797	0.17775553223290427	No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG	1791	0.177162024612761	No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC	1725	0.17063344079118523	No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG	1619	0.16014813950198775	No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG	1619	0.16014813950198775	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	1606	0.1588622063250107	No Hit
GGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCCCAGAG	1551	0.1534217198070309	No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT	1537	0.1520368686933633	No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC	1518	0.150157427896243	No Hit
GTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGACCCCT	1371	0.1356164912027333	No Hit
GTGTAGAAGAAGCCTCGTTCCCCGCACACTAGGTAGAGAGCTT	1333	0.13185760960849272	No Hit
GCACAGGTGTTGGTTCACAAAGGCTGCGGCTGGGTCAGGTCCC	1302	0.1287911535710859	No Hit
GGTCCCCAGAGGGCCAGCAGCGCCAGCAGGGGCAGGAGGCGCA	1296	0.12819764595094263	No Hit
TTGTGGAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCT	1168	0.11553615005455324	No Hit
CTAGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTG	1166	0.11533831418117217	No Hit
TGCTGGTACAGCATTGTTCCACAATGCCACGCTTCTGCAGGGA	1160	0.11474480656102891	No Hit
GAACAATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGA	1141	0.11286536576390861	No Hit
CTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCCA	1126	0.11138159671355047	No Hit
CACCTGGTGGAAGCTCTCTACCTAGTGTGCGGGGAACGAGGCT	1119	0.11068917115671667	No Hit
GCCCTGTGGATGCGCCTCCTGCCCCTGCTGGCGCTGCTGGCCC	1114	0.11019458147326397	No Hit
TTGCAGTAGTTCTCCAGCTGGTAGAGGGAGCAGATGCTGGTAC	1027	0.1015887209811868	No Hit
CCACAGGGCCATGGCAGAAGGACAGTGATCTGCTTGATGGCCT	1013	0.10020386986751921	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.891793669054216E-5	0.0
2	0.0	0.0	0.0	9.891793669054216E-5	0.0
3	0.0	0.0	0.0	9.891793669054216E-5	0.0
4	0.0	0.0	0.0	9.891793669054216E-5	0.0
5	1.9783587338108432E-4	0.0	0.0	9.891793669054216E-5	0.0
6	1.9783587338108432E-4	0.0	0.0	9.891793669054216E-5	0.0
7	1.9783587338108432E-4	0.0	0.0	9.891793669054216E-5	0.0
8	1.9783587338108432E-4	0.0	0.0	9.891793669054216E-5	0.0
9	1.9783587338108432E-4	0.0	0.0	2.967538100716265E-4	0.0
10	2.967538100716265E-4	0.0	0.0	2.967538100716265E-4	0.0
11	3.9567174676216865E-4	0.0	0.0	2.967538100716265E-4	0.0
12	3.9567174676216865E-4	0.0	0.0	2.967538100716265E-4	0.0
13	4.945896834527108E-4	0.0	0.0	4.945896834527108E-4	0.0
14	4.945896834527108E-4	0.0	0.0	5.93507620143253E-4	0.0
15	4.945896834527108E-4	0.0	0.0	5.93507620143253E-4	0.0
16	4.945896834527108E-4	0.0	0.0	5.93507620143253E-4	0.0
17	7.913434935243373E-4	0.0	0.0	8.902614302148794E-4	0.0
18	8.902614302148794E-4	0.0	0.0	9.891793669054216E-4	0.0
19	8.902614302148794E-4	0.0	0.0	0.001187015240286506	0.0
20	9.891793669054216E-4	0.0	0.0	0.001187015240286506	0.0
21	9.891793669054216E-4	0.0	0.0	0.001285933176977048	0.0
22	0.0010880973035959638	0.0	0.0	0.001978358733810843	0.0
23	0.0010880973035959638	0.0	0.0	0.0022751125438824696	0.0
24	0.0010880973035959638	0.0	0.0	0.003264291910787891	0.0
25	0.0010880973035959638	0.0	0.0	0.00415455334100277	0.0
26	0.0010880973035959638	0.0	0.0	0.007122091441719035	0.0
27	0.001187015240286506	0.0	0.0	0.018992243844584096	0.0
28	0.001187015240286506	0.0	0.0	0.050547065648867046	0.0
29	0.001187015240286506	0.0	0.0	0.09911577256392325	0.0
30	0.001285933176977048	0.0	0.0	0.16222541617248915	0.0
31	0.001285933176977048	0.0	0.0	0.33285885696367434	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAGCGGG	45	1.3230582E-4	24.666666	28
ACAATCG	40	0.0019310269	23.125002	37
GGTATCA	530	0.0	22.688679	1
GGCGTAA	55	5.142505E-4	20.181818	13
TGCATCA	1200	0.0	19.733334	14
CAGGACA	1305	0.0	19.70498	4
GACAGGC	1270	0.0	19.519686	7
GCATCAG	1215	0.0	19.489712	15
CCAGGAC	1435	0.0	18.69338	3
GCTGCAT	1280	0.0	18.644531	12
TTTAGCG	70	1.2190125E-4	18.5	26
TAGAGTA	50	0.007034309	18.5	5
GTATTAT	80	1.6163563E-5	18.5	1
ACAGGCT	1350	0.0	18.5	8
TATAGAC	80	1.6163563E-5	18.5	3
GCGTAAA	60	9.235767E-4	18.5	14
GGACAGG	1410	0.0	18.237589	6
TCCAGGA	1485	0.0	17.690235	2
CTGCATC	1350	0.0	17.540741	13
GCCGATC	85	2.72259E-5	17.411764	24
>>END_MODULE