FastQCFastQC Report
Fri 10 Feb 2017
ERR1630241.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630241.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2316105
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT53900.23271829213269693No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51000.22019727084911953No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41780.18038905835443556No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24910.1075512552323837No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA17050.023.2199421
ACCGTCG1401.87174E-917.1785728
ATACCGT2050.017.1463436
GCGACTT1201.9374675E-615.41666722
CGTCGTA1455.35274E-815.31034510
GTTAGAC855.367071E-415.2352943
CGCCGAT1001.0941253E-414.79999937
GTATCAA27300.014.5695972
CTAATAC3950.014.0506343
CGAATTA1854.9058144E-914.00000115
GTCCCGT800.006301166513.8750016
CGGGTAA2009.858923E-1013.87499924
AAGACGG3750.013.8133335
CTTATAC8850.013.7966137
TAAGACT2700.013.7037044
TACCGTC1907.1358954E-913.6315797
TCTTATA14300.013.58391737
TATACTG3050.013.3442615
GACGGAC3350.013.2537327
TACACCA9850.013.1472075
Produced by FastQC (version 0.11.5)