##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630241.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2316105
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.658193821091878	31.0	31.0	34.0	30.0	34.0
2	31.866678755928596	33.0	31.0	34.0	30.0	34.0
3	31.99127414344341	33.0	31.0	34.0	30.0	34.0
4	35.67934182603984	37.0	35.0	37.0	33.0	37.0
5	35.58095854894316	37.0	35.0	37.0	33.0	37.0
6	35.641942398984504	37.0	35.0	37.0	33.0	37.0
7	35.62931905073388	37.0	35.0	37.0	33.0	37.0
8	35.62258187776461	37.0	35.0	37.0	33.0	37.0
9	37.34353710216074	39.0	37.0	39.0	34.0	39.0
10	37.193958391350996	39.0	37.0	39.0	34.0	39.0
11	37.281314534531035	39.0	37.0	39.0	34.0	39.0
12	37.20280254997075	39.0	37.0	39.0	34.0	39.0
13	37.21114500422045	39.0	37.0	39.0	34.0	39.0
14	38.410205927624176	40.0	38.0	41.0	34.0	41.0
15	38.41130259638488	40.0	38.0	41.0	34.0	41.0
16	38.362639863045935	40.0	38.0	41.0	34.0	41.0
17	38.32896867801762	40.0	38.0	41.0	34.0	41.0
18	38.28078433404358	40.0	38.0	41.0	33.0	41.0
19	38.313866599312206	40.0	38.0	41.0	34.0	41.0
20	38.29133394211402	40.0	38.0	41.0	34.0	41.0
21	38.22923010830684	40.0	38.0	41.0	34.0	41.0
22	38.19868140693103	40.0	38.0	41.0	34.0	41.0
23	38.13911674988828	40.0	38.0	41.0	33.0	41.0
24	38.09827879133286	40.0	37.0	41.0	33.0	41.0
25	38.07933016853726	40.0	37.0	41.0	33.0	41.0
26	37.90108436361909	40.0	37.0	41.0	33.0	41.0
27	37.786142683513916	40.0	37.0	41.0	33.0	41.0
28	37.717594409579874	40.0	37.0	41.0	32.0	41.0
29	37.6550652928084	40.0	37.0	41.0	32.0	41.0
30	37.59064507006375	40.0	37.0	41.0	32.0	41.0
31	37.54274525550439	40.0	37.0	41.0	32.0	41.0
32	37.446777671996735	40.0	37.0	41.0	32.0	41.0
33	37.36564015880109	39.0	36.0	41.0	32.0	41.0
34	37.30207093374437	39.0	36.0	41.0	31.0	41.0
35	37.16741598502659	39.0	36.0	41.0	31.0	41.0
36	37.090425520431936	39.0	36.0	41.0	31.0	41.0
37	37.03002281848189	39.0	36.0	41.0	31.0	41.0
38	36.92179499634084	39.0	36.0	41.0	30.0	41.0
39	36.83571383853496	39.0	36.0	40.0	30.0	41.0
40	36.74458239155824	39.0	35.0	40.0	30.0	41.0
41	36.63115661854709	39.0	35.0	40.0	30.0	41.0
42	36.60315616088217	39.0	35.0	40.0	30.0	41.0
43	35.35765649657507	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	6.0
11	2.0
12	1.0
13	5.0
14	6.0
15	8.0
16	22.0
17	66.0
18	124.0
19	239.0
20	444.0
21	895.0
22	1576.0
23	2828.0
24	4514.0
25	7096.0
26	10321.0
27	15271.0
28	21745.0
29	29460.0
30	39700.0
31	51873.0
32	66485.0
33	85507.0
34	111904.0
35	150208.0
36	210091.0
37	322001.0
38	598791.0
39	584910.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.21760239712793	19.23194328409118	12.347799430509411	26.20265488827147
2	18.348088709276997	21.6248399791892	34.571316930795454	25.455754380738348
3	19.74513245297601	23.582782300457016	29.753443820552178	26.918641426014794
4	14.781151977134025	16.14551153768935	34.88796924146358	34.185367243713046
5	14.394597826955168	36.63896930406869	34.49623397902945	14.470198889946701
6	33.793588805343454	35.55680765768391	16.173316840125988	14.476286696846646
7	29.06396730718167	30.681899136697172	21.769349835175866	18.484783720945295
8	26.95983126844422	34.01426101148264	20.443632736857783	18.582274983215356
9	27.078953674380045	14.374261961353222	19.60135658789217	38.94542777637456
10	16.55956875875662	26.80884502213846	33.8263161644226	22.80527005468232
11	34.82018302279042	22.199986615460006	21.866625217768622	21.113205143980952
12	21.12158127546031	25.485675304012556	30.252557634476844	23.14018578605029
13	29.780256076473215	20.18345454977214	25.525699396184542	24.510589977570103
14	22.870249837550542	20.801388538084414	25.602854792852657	30.72550683151239
15	24.846973690743727	27.605613735128586	22.638740471610745	24.90867210251694
16	24.518577525630313	26.914107952791433	24.23573197242785	24.331582549150404
17	23.184527471768334	26.768518698418248	26.026151664108493	24.02080216570492
18	23.302397775575805	25.86929349058009	26.92308854736724	23.905220186476868
19	24.133102773837976	25.52241802508954	27.07662217386517	23.267857027207317
20	24.622804233832234	25.198857564747712	26.608335977859383	23.570002223560678
21	24.30351819110101	25.431144097525802	26.049639372999067	24.21569833837412
22	24.353127340945253	25.33969746622023	26.282400841067222	24.0247743517673
23	23.975683313148583	25.56434185842179	26.467150668903177	23.992824159526446
24	23.836699976900874	25.757985928962633	26.52712204325797	23.87819205087852
25	24.404420352272457	25.44552168403419	26.233309802448506	23.91674816124485
26	23.88233694068274	26.149116728300314	26.249975713536305	23.71857061748064
27	24.38835890428111	25.56015379268211	25.782639388110645	24.268847914926138
28	23.94079715729641	25.7006914626064	26.39366522674922	23.96484615334797
29	23.509599089851278	25.739074869230887	26.744253822689384	24.007072218228448
30	23.618488799082943	26.467409724515946	26.387016132688284	23.527085343712827
31	24.074642557224305	25.99420147186764	26.235080015802392	23.696075955105663
32	23.492890002828023	25.882807558379263	26.561015152594553	24.063287286198165
33	23.42976678518461	25.863248859615606	26.74507416546314	23.96191018973665
34	24.22938510991514	25.251359502267817	26.64507869893636	23.874176688880684
35	23.931427979301457	25.565550784614686	27.080896591475774	23.42212464460808
36	23.670688505054823	25.660710546369874	26.637954669585362	24.03064627898994
37	24.174767551557462	25.06691190598008	26.651036978029925	24.107283564432528
38	23.74957093914136	25.205463482873185	27.023774828861384	24.02119074912407
39	23.71334632928991	25.31016512636517	26.97498602179089	24.00150252255403
40	23.13707711869712	25.160171926575003	27.550521241480848	24.152229713247024
41	22.848920925433	24.93116676489192	28.03568922825174	24.184223081423337
42	23.30900369370128	24.864978055830804	27.593179065715933	24.232839184751988
43	23.01985445392156	24.455195252374136	28.064487577203966	24.46046271650033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	605.0
1	516.0
2	427.0
3	823.0
4	1219.0
5	1219.0
6	1591.5
7	1964.0
8	1821.0
9	1678.0
10	2518.5
11	3359.0
12	3359.0
13	5514.5
14	7670.0
15	10575.0
16	13480.0
17	13970.0
18	14460.0
19	14460.0
20	18458.5
21	22457.0
22	23884.0
23	25311.0
24	29870.5
25	34430.0
26	34430.0
27	39759.0
28	45088.0
29	52296.5
30	59505.0
31	69129.0
32	78753.0
33	78753.0
34	85453.0
35	92153.0
36	100662.5
37	109172.0
38	115414.0
39	121656.0
40	121656.0
41	127528.0
42	133400.0
43	134521.5
44	135643.0
45	141416.5
46	147190.0
47	147190.0
48	148609.0
49	150028.0
50	157917.0
51	165806.0
52	174030.5
53	182255.0
54	182255.0
55	173071.5
56	163888.0
57	155449.0
58	147010.0
59	132167.0
60	117324.0
61	117324.0
62	108991.0
63	100658.0
64	88920.0
65	77182.0
66	66946.0
67	56710.0
68	56710.0
69	47600.5
70	38491.0
71	33615.0
72	28739.0
73	23123.0
74	17507.0
75	17507.0
76	13487.0
77	9467.0
78	7733.0
79	5999.0
80	4344.0
81	2689.0
82	2689.0
83	1947.5
84	1206.0
85	960.0
86	714.0
87	570.5
88	427.0
89	427.0
90	359.0
91	291.0
92	174.5
93	58.0
94	42.0
95	26.0
96	26.0
97	16.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2316105.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	57.84226917364609
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.14326852324511	48.091953178982806
2	9.9366392199276	11.49515520880922
3	2.7948227400245855	4.849766676633875
4	1.22879210251601	2.843044942087263
5	0.690629338834762	1.9973784058048765
6	0.4081064606729307	1.4163482249848602
7	0.2886396383908777	1.1686900160592313
8	0.204082925375356	0.9443695602645176
9	0.16441592716413575	0.8559171283916277
>10	0.9663755997008892	10.6343374858487
>50	0.09707474249718982	3.862072659390757
>100	0.06951711794785344	7.714697866216315
>500	0.005988755845204588	2.33501755653615
>1k	0.0014971889613010789	1.3370298577814168
>5k	1.4971889613010788E-4	0.45422123220827326
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5390	0.23271829213269693	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5100	0.22019727084911953	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4178	0.18038905835443556	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2491	0.1075512552323837	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	8.635187092122335E-5	0.0
6	0.0	0.0	0.0	8.635187092122335E-5	0.0
7	4.3175935460611675E-5	0.0	0.0	8.635187092122335E-5	0.0
8	4.3175935460611675E-5	0.0	0.0	8.635187092122335E-5	0.0
9	4.3175935460611675E-5	0.0	0.0	8.635187092122335E-5	0.0
10	8.635187092122335E-5	0.0	0.0	1.2952780638183503E-4	0.0
11	8.635187092122335E-5	0.0	0.0	1.2952780638183503E-4	0.0
12	8.635187092122335E-5	0.0	0.0	2.1587967730305838E-4	0.0
13	8.635187092122335E-5	0.0	0.0	3.454074836848934E-4	0.0
14	8.635187092122335E-5	0.0	0.0	3.8858341914550505E-4	0.0
15	8.635187092122335E-5	0.0	0.0	4.3175935460611675E-4	0.0
16	8.635187092122335E-5	0.0	0.0	6.044630964485634E-4	0.0
17	8.635187092122335E-5	0.0	0.0	7.771668382910101E-4	0.0
18	8.635187092122335E-5	0.0	0.0	8.635187092122335E-4	0.0
19	8.635187092122335E-5	0.0	0.0	9.930465155940686E-4	0.0
20	8.635187092122335E-5	0.0	0.0	9.930465155940686E-4	0.0
21	8.635187092122335E-5	0.0	0.0	0.0011225743219759035	0.0
22	1.2952780638183503E-4	0.0	0.0	0.0015543336765820202	0.0
23	1.2952780638183503E-4	0.0	0.0	0.0020724449021093604	0.0
24	1.2952780638183503E-4	0.0	0.0	0.0035404267077701573	0.0
25	1.2952780638183503E-4	0.0	0.0	0.004317593546061167	0.0
26	1.2952780638183503E-4	0.0	0.0	0.006217334706328081	0.0
27	1.2952780638183503E-4	0.0	0.0	0.01830659663529935	0.0
28	1.2952780638183503E-4	0.0	0.0	0.04481662100811492	0.0
29	1.2952780638183503E-4	0.0	0.0	0.08445212976095644	0.0
30	1.2952780638183503E-4	0.0	0.0	0.13557243734632066	0.0
31	1.2952780638183503E-4	0.0	0.0	0.28120486765496383	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1705	0.0	23.219942	1
ACCGTCG	140	1.87174E-9	17.178572	8
ATACCGT	205	0.0	17.146343	6
GCGACTT	120	1.9374675E-6	15.416667	22
CGTCGTA	145	5.35274E-8	15.310345	10
GTTAGAC	85	5.367071E-4	15.235294	3
CGCCGAT	100	1.0941253E-4	14.799999	37
GTATCAA	2730	0.0	14.569597	2
CTAATAC	395	0.0	14.050634	3
CGAATTA	185	4.9058144E-9	14.000001	15
GTCCCGT	80	0.0063011665	13.875001	6
CGGGTAA	200	9.858923E-10	13.874999	24
AAGACGG	375	0.0	13.813333	5
CTTATAC	885	0.0	13.79661	37
TAAGACT	270	0.0	13.703704	4
TACCGTC	190	7.1358954E-9	13.631579	7
TCTTATA	1430	0.0	13.583917	37
TATACTG	305	0.0	13.344261	5
GACGGAC	335	0.0	13.253732	7
TACACCA	985	0.0	13.147207	5
>>END_MODULE