FastQCFastQC Report
Fri 10 Feb 2017
ERR1630240.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630240.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2367773
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC91910.38817065656209443No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT80430.3396862790478648No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT68290.28841447216435023No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT55680.23515767769967813No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT53120.2243458304491182No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG40270.17007542530470615No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT35600.1503522508280988No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG35060.14807162679868383No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG34950.14760705523713635No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT33600.14190549516359888No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT33340.14080741692721388No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG32000.13514809063199892No Hit
GCTTTATTCCATCTCTCTCGGTGCAGGAGGCGGCGGGTGGGGG29340.12391390559821401No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT28110.11871915086454655No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG27950.11804341041138657No Hit
ATGCTGTACCAGCATCTGCTCCCTCTACCAGCTGGAGAACTAC26980.11394673391410409No Hit
CTGCAGAAGCGTGGCATTGTGGAACAATGCTGTACCAGCATCT26760.1130175907910091No Hit
ACCAACACCTGTGCGGCTCACACCTGGTGGAAGCTCTCTACCT26270.11094813565320663No Hit
GGTGTGAGCCGCACAGGTGTTGGTTCACAAAGGCTGCGGCTGG25130.10613348492444166No Hit
CTGGGGACCTGACCCAGCCGCAGCCTTTGTGAACCAACACCTG24960.10541551069295917No Hit
TCTCTACCTAGTGTGCGGGGAACGAGGCTTCTTCTACACACCC24200.10220574354044917No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTAACG1153.0559022E-919.30434830
TAGCGAC500.00703703318.4999984
TGCATCA24650.017.8620714
AACGCTA1258.591087E-917.76000233
CCAGGAC29000.017.4155163
CGGATCG852.72509E-517.41176437
GCTGCAT25350.017.36883712
GCATCAG25500.017.26666615
GACAGGC26200.017.2290087
TAGGGCG650.001580602717.0769234
CAGGACA28650.016.8534034
CTGCATC26650.016.66041213
TCTAGCG1901.8189894E-1216.55263128
CGCTAAG1251.6597733E-716.2835
TCCAGGA30650.016.2365422
GGACAGG29500.016.179666
ACAGGCT27950.016.084088
ATCAAGC28100.015.80071230
AGTACCG1201.937513E-615.4166665
AGAGGCC28150.015.3783323
Produced by FastQC (version 0.11.5)