FastQCFastQC Report
Fri 10 Feb 2017
ERR1630239.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630239.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2726627
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC101940.37386851960315803No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT90260.33103171060801495No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT78490.2878648234613682No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT65570.24048027104550787No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT52370.19206880882496946No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41010.15040561103517278No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT35640.1307109479954537No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG34320.12586980177339988No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG33850.12414606031554737No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT32670.11981836899583258No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG31790.11659093818113002No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG28970.10624848943401499No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT28610.10492817682800032No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG27450.10067383620861967No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC802.7292117E-823.12528
GACAGGC25350.018.463517
GCTGCAT24450.018.38650312
TGCATCA25250.018.09702914
GGTATCA29900.018.0668891
CCAGGAC28500.017.7210523
GCATCAG25900.017.71428715
TTTAGCG1054.8032416E-717.61904726
CAGGACA27850.017.5368044
CTTATAC19800.017.09848437
ACAGGCT27900.017.041228
TCCAGGA29750.016.9142862
CTGCATC27050.016.75600813
GGACAGG29450.016.4584056
GGCCATC27800.016.4370526
ATCAAGC27550.016.38475630
TCTAGCG2550.015.96078428
GTACCGA700.002593716815.8571436
AGAGGCC29200.015.648972523
CATCAAG29050.015.47504329
Produced by FastQC (version 0.11.5)