##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630239.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2726627
Sequences flagged as poor quality	0
Sequence length	43
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.861232944586845	33.0	31.0	34.0	30.0	34.0
2	32.070011409701436	33.0	31.0	34.0	30.0	34.0
3	32.18922390191251	34.0	31.0	34.0	30.0	34.0
4	35.832872996563154	37.0	35.0	37.0	35.0	37.0
5	35.76502579927507	37.0	35.0	37.0	35.0	37.0
6	35.82014518304117	37.0	35.0	37.0	35.0	37.0
7	35.79626256176587	37.0	35.0	37.0	35.0	37.0
8	35.79363037188438	37.0	35.0	37.0	35.0	37.0
9	37.54499166919421	39.0	37.0	39.0	35.0	39.0
10	37.43653972472216	39.0	37.0	39.0	34.0	39.0
11	37.50558877323521	39.0	37.0	39.0	35.0	39.0
12	37.43192853294565	39.0	37.0	39.0	34.0	39.0
13	37.438866408936754	39.0	37.0	39.0	34.0	39.0
14	38.6773566754822	40.0	38.0	41.0	35.0	41.0
15	38.68242520887529	40.0	38.0	41.0	35.0	41.0
16	38.652548368368684	40.0	38.0	41.0	34.0	41.0
17	38.62390271936719	40.0	38.0	41.0	34.0	41.0
18	38.54522419091427	40.0	38.0	41.0	34.0	41.0
19	38.584816698433634	40.0	38.0	41.0	34.0	41.0
20	38.56405148192253	40.0	38.0	41.0	34.0	41.0
21	38.52005829913663	40.0	38.0	41.0	34.0	41.0
22	38.472875461146685	40.0	38.0	41.0	34.0	41.0
23	38.41842723628864	40.0	38.0	41.0	34.0	41.0
24	38.37981579438625	40.0	38.0	41.0	34.0	41.0
25	38.364065565257	40.0	38.0	41.0	34.0	41.0
26	38.18397309202909	40.0	38.0	41.0	33.0	41.0
27	38.07267477363057	40.0	38.0	41.0	33.0	41.0
28	37.99482694185893	40.0	37.0	41.0	33.0	41.0
29	37.93271430232298	40.0	37.0	41.0	33.0	41.0
30	37.871681751849444	40.0	37.0	41.0	33.0	41.0
31	37.81643143708325	40.0	37.0	41.0	33.0	41.0
32	37.735430992211256	40.0	37.0	41.0	33.0	41.0
33	37.64035088041012	40.0	37.0	41.0	32.0	41.0
34	37.56394805743506	40.0	37.0	41.0	32.0	41.0
35	37.42481168124573	40.0	37.0	41.0	32.0	41.0
36	37.340753612430305	40.0	36.0	41.0	31.0	41.0
37	37.26045293323949	40.0	36.0	41.0	31.0	41.0
38	37.162583294304646	40.0	36.0	41.0	31.0	41.0
39	37.09061782194631	39.0	36.0	41.0	31.0	41.0
40	36.97224812928208	39.0	36.0	41.0	31.0	41.0
41	36.84055758268366	39.0	35.0	41.0	30.0	41.0
42	36.80704768198951	39.0	35.0	41.0	30.0	41.0
43	35.581650882207214	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	5.0
10	8.0
11	6.0
12	17.0
13	6.0
14	5.0
15	7.0
16	22.0
17	53.0
18	109.0
19	215.0
20	453.0
21	837.0
22	1524.0
23	2807.0
24	4493.0
25	6986.0
26	10922.0
27	15787.0
28	22092.0
29	30850.0
30	41420.0
31	54767.0
32	70781.0
33	92028.0
34	123589.0
35	164107.0
36	226847.0
37	345181.0
38	633055.0
39	877646.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.09167590579863	18.700027543187975	12.43000967862491	26.778286872388485
2	18.004589553319907	20.22003743086238	35.97066998896439	25.804703026853325
3	19.516274136506386	23.029809357862298	29.487018209678112	27.966898295953207
4	14.34211573493551	15.389380358956323	34.73889167825302	35.529612227855154
5	14.580578861721827	36.842186334984575	33.75456195511891	14.822672848174687
6	34.68926992947697	35.01670745576861	15.843934648927045	14.450087965827377
7	29.713085068107958	30.220671914420272	21.507012143575192	18.559230873896578
8	27.428614181551055	34.387688525053115	19.537472488902957	18.646224804492878
9	27.273367424293827	14.227835343814904	19.348411058791687	39.150386173099584
10	17.409678698259793	26.69877471322627	32.37758593309609	23.513960655417847
11	35.88070535500456	22.01606600389419	21.42507941130195	20.67814922979931
12	22.285519801571684	25.08700309943384	29.369987167294976	23.257489931699496
13	29.887256306051395	19.20710093459795	25.271150032622725	25.63449272672793
14	22.61215047015965	20.17151594259134	25.713454755637642	31.502878831611365
15	25.304891354776434	27.165835297603962	22.335801706650745	25.193471640968863
16	24.54919576458386	25.93974166616849	23.74461193261858	25.766450636629067
17	23.299886636492634	26.71271134628976	25.776132929073174	24.211269088144434
18	23.586614524098824	24.930912809122773	26.768897982745717	24.71357468403269
19	24.44881533117658	24.99201394250112	26.40848931665387	24.15068140966843
20	24.267309023199726	25.478659163868034	26.22107827730012	24.03295353563212
21	26.00109952699801	24.72571422493799	25.497620319904406	23.77556592815959
22	25.264988573794657	25.11146555799528	25.767844299935415	23.855701568274647
23	24.424866327517478	25.399623784257987	25.665263345518106	24.51024654270643
24	24.57816929121585	25.40182430526801	25.832282890178966	24.187723513337176
25	24.586787998505113	24.898675176325916	26.209635568047997	24.304901257120978
26	24.84971358385287	25.417668056540187	26.118130569381144	23.614487790225798
27	25.00444688620776	25.206968169830347	25.357887235767855	24.430697708194042
28	24.02191425523183	24.782047562794617	26.755584830635065	24.440453351338483
29	23.914932258794476	24.886498960070448	26.932103290989197	24.266465490145883
30	23.74142117715404	25.565433042363328	26.446595005477462	24.24655077500516
31	23.975739989371483	25.35278936209463	26.23699537927263	24.434475269261252
32	23.506112130482094	25.118323848476525	26.502488239132084	24.873075781909296
33	24.094934877414474	25.34842499542475	26.22335214901048	24.333287978150295
34	24.705102678144094	25.193141562817356	26.13932892177771	23.962426837260836
35	24.76983467118898	25.026855525159842	26.67233178575581	23.53097801789537
36	24.32118511259516	25.38128610917445	25.478842540618867	24.818686237611526
37	23.805089585044087	24.66692363862017	26.681904052149413	24.84608272418633
38	24.195095258720755	24.602778451177958	26.938888230770104	24.26323805933118
39	23.066961487581544	24.71848918095508	27.39454278124584	24.820006550217542
40	23.71688536789227	23.888049227122007	28.252672624455048	24.14239278053067
41	22.492735530015658	24.237381937463393	28.406085614203924	24.86379691831703
42	22.681540232675758	24.816742444052668	27.728435169166886	24.773282154104688
43	22.593812795076115	23.33832240346773	28.768988204107128	25.298876597349036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1102.0
1	820.5
2	539.0
3	1218.5
4	1898.0
5	1898.0
6	2399.5
7	2901.0
8	2631.5
9	2362.0
10	3378.5
11	4395.0
12	4395.0
13	7832.5
14	11270.0
15	15998.0
16	20726.0
17	19747.0
18	18768.0
19	18768.0
20	22513.5
21	26259.0
22	25996.5
23	25734.0
24	30304.0
25	34874.0
26	34874.0
27	40541.5
28	46209.0
29	53561.0
30	60913.0
31	70073.0
32	79233.0
33	79233.0
34	90941.0
35	102649.0
36	114919.5
37	127190.0
38	134300.5
39	141411.0
40	141411.0
41	149962.0
42	158513.0
43	162622.5
44	166732.0
45	170589.5
46	174447.0
47	174447.0
48	175161.0
49	175875.0
50	180760.0
51	185645.0
52	190853.0
53	196061.0
54	196061.0
55	194733.0
56	193405.0
57	180667.0
58	167929.0
59	152482.5
60	137036.0
61	137036.0
62	129535.5
63	122035.0
64	106240.0
65	90445.0
66	76585.0
67	62725.0
68	62725.0
69	54365.0
70	46005.0
71	41013.0
72	36021.0
73	38437.5
74	40854.0
75	40854.0
76	38451.5
77	36049.0
78	27576.0
79	19103.0
80	12025.0
81	4947.0
82	4947.0
83	3380.5
84	1814.0
85	1462.0
86	1110.0
87	945.0
88	780.0
89	780.0
90	635.0
91	490.0
92	300.5
93	111.0
94	78.0
95	45.0
96	45.0
97	27.5
98	10.0
99	8.5
100	7.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2726627.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.74842734601717
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.36161102747745	38.6875372923586
2	11.954468196427248	11.655230486676132
3	3.6807007738352318	5.38285122767208
4	1.5260828406685811	2.975765539293433
5	0.8107775563179126	1.9762065398972555
6	0.502691119412218	1.4703240907292734
7	0.34534335917764647	1.1784461958010708
8	0.23349919060854063	0.9106174662987406
9	0.1767558604041296	0.7754913197004119
>10	1.143380434718811	10.777753863313048
>50	0.13959403079080923	4.750191168521537
>100	0.10893450743469169	10.270450744938923
>500	0.010874760713249089	3.624268074523095
>1k	0.004908746155284156	4.134147632376823
>5k	3.0207668647902496E-4	1.055432277555154
>10k+	7.551917161975624E-5	0.3752860803445268
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CTCCAGGACAGGCTGCATCAGAAGAGGCCATCAAGCAGATCAC	10194	0.37386851960315803	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9026	0.33103171060801495	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7849	0.2878648234613682	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6557	0.24048027104550787	No Hit
GTGCAGGAGGCGGCGGGTGGGGGGCTGCCTGCGGGCTGCGTCT	5237	0.19206880882496946	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4101	0.15040561103517278	No Hit
AGGTAGAGAGCTTCCACCAGGTGTGAGCCGCACAGGTGTTGGT	3564	0.1307109479954537	No Hit
CTTCTACACACCCAAGACCCGCCGGGAGGCAGAGGACCTGCAG	3432	0.12586980177339988	No Hit
CTCCAGGGCCAAGGGCTGCAGGCTGCCTGCACCAGGGCCCCCG	3385	0.12414606031554737	No Hit
CTTCTGCAGGGACCCCTCCAGGGCCAAGGGCTGCAGGCTGCCT	3267	0.11981836899583258	No Hit
GGTCTTGGGTGTGTAGAAGAAGCCTCGTTCCCCGCACACTAGG	3179	0.11659093818113002	No Hit
CCCAAGACCCGCCGGGAGGCAGAGGACCTGCAGGTGGGGCAGG	2897	0.10624848943401499	No Hit
GCCCTGGAGGGGTCCCTGCAGAAGCGTGGCATTGTGGAACAAT	2861	0.10492817682800032	No Hit
CATCAGAAGAGGCCATCAAGCAGATCACTGTCCTTCTGCCATG	2745	0.10067383620861967	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	3.667535016707456E-5	0.0
2	0.0	0.0	0.0	7.335070033414912E-5	0.0
3	0.0	0.0	0.0	7.335070033414912E-5	0.0
4	0.0	0.0	0.0	7.335070033414912E-5	0.0
5	0.0	0.0	0.0	7.335070033414912E-5	0.0
6	0.0	0.0	0.0	7.335070033414912E-5	0.0
7	0.0	0.0	0.0	7.335070033414912E-5	0.0
8	0.0	0.0	0.0	7.335070033414912E-5	0.0
9	0.0	0.0	0.0	1.1002605050122368E-4	0.0
10	0.0	0.0	0.0	2.567274511695219E-4	0.0
11	0.0	0.0	0.0	4.7677955217196926E-4	0.0
12	0.0	0.0	0.0	4.7677955217196926E-4	0.0
13	0.0	0.0	0.0	4.7677955217196926E-4	0.0
14	0.0	0.0	0.0	5.501302525061184E-4	0.0
15	0.0	0.0	0.0	6.234809528402674E-4	0.0
16	0.0	0.0	0.0	8.435330538427148E-4	0.0
17	1.4670140066829824E-4	0.0	0.0	9.168837541768639E-4	0.0
18	1.4670140066829824E-4	0.0	0.0	0.001173611205346386	0.0
19	1.8337675083537279E-4	0.0	0.0	0.0014303386565159078	0.0
20	1.8337675083537279E-4	0.0	0.0	0.0016137154073512805	0.0
21	1.8337675083537279E-4	0.0	0.0	0.001907118208687877	0.0
22	1.8337675083537279E-4	0.0	0.0	0.0026773005621964427	0.0
23	1.8337675083537279E-4	0.0	0.0	0.003997613168211127	0.0
24	2.2005210100244736E-4	0.0	0.0	0.005868056026731929	0.0
25	2.2005210100244736E-4	0.0	0.0	0.0072250439829136875	0.0
26	2.2005210100244736E-4	0.0	0.0	0.011039280400289442	0.0
27	2.2005210100244736E-4	0.0	0.0	0.02945030618416087	0.0
28	2.934028013365965E-4	0.0	0.0	0.0775683656033627	0.0
29	2.934028013365965E-4	0.0	0.0	0.14941537658066176	0.0
30	3.30078151503671E-4	0.0	0.0	0.24873222483309965	0.0
31	3.30078151503671E-4	0.0	0.0	0.5313891485707433	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGCGAAC	80	2.7292117E-8	23.125	28
GACAGGC	2535	0.0	18.46351	7
GCTGCAT	2445	0.0	18.386503	12
TGCATCA	2525	0.0	18.097029	14
GGTATCA	2990	0.0	18.066889	1
CCAGGAC	2850	0.0	17.721052	3
GCATCAG	2590	0.0	17.714287	15
TTTAGCG	105	4.8032416E-7	17.619047	26
CAGGACA	2785	0.0	17.536804	4
CTTATAC	1980	0.0	17.098484	37
ACAGGCT	2790	0.0	17.04122	8
TCCAGGA	2975	0.0	16.914286	2
CTGCATC	2705	0.0	16.756008	13
GGACAGG	2945	0.0	16.458405	6
GGCCATC	2780	0.0	16.43705	26
ATCAAGC	2755	0.0	16.384756	30
TCTAGCG	255	0.0	15.960784	28
GTACCGA	70	0.0025937168	15.857143	6
AGAGGCC	2920	0.0	15.6489725	23
CATCAAG	2905	0.0	15.475043	29
>>END_MODULE