FastQCFastQC Report
Fri 10 Feb 2017
ERR1630238.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630238.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2348006
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT70780.3014472705776732No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT61680.2626909811985148No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT48570.206856370895134No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32600.13884121250116055No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACCGA502.7032653E-422.26
TATACCG656.9060494E-519.9230775
AACCGTA609.2407275E-418.57
CGTTATA752.0684229E-417.2666682
GGTATCA29550.016.7783411
CGAACGA1802.0190782E-1015.41666616
GTACTAG1203.3032415E-513.8749991
ACGAACG2051.4260877E-913.53658515
GTATTAC2651.8189894E-1213.2641521
GTCTTAC1409.568166E-613.2142861
AGGTCGC3250.013.09230710
CGTTCAA850.0094085513.05882327
CTTATAC9350.013.05882337
TCTCGTA850.0094085513.05882312
CGCACTA850.0094085513.05882329
TAACACG1153.5796425E-412.8695654
ACACCGT1050.00264498412.3333346
TCGCCAT3750.012.33333313
CCGATAA2401.1295924E-912.3333339
TCGGCGA1205.124977E-412.33333314
Produced by FastQC (version 0.11.5)