FastQCFastQC Report
Fri 10 Feb 2017
ERR1630237.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630237.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences139030
Sequences flagged as poor quality0
Sequence length43
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT35802.5749838164424945No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT33382.4009206646047616No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT22281.6025318276630944No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14321.0299935265769977No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA4630.33302165000359635No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT4600.33086384233618643No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4100.2949003812126879No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA3850.2769186506509386No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3490.25102495864201974No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3390.24383226641732No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA3180.22872761274545061No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA3130.2251312666331008No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA3070.22081565129828096No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2590.18629072861972237No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA2500.17981730561749262No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC2400.17262461339279292No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT2380.17118607494785298No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA2210.1589584981658635No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA2200.1582392289433935No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG2190.15751995972092353No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2180.15680069049845358No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC2090.15032726749622383No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA2040.14673092138387397No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA2040.14673092138387397No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA2010.14457311371646409No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1950.14025749838164425No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC1920.13809969071423434No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1860.13378407537941453No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA1830.1316262677120046No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1830.1316262677120046No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT1760.1265913831547148No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA1750.12587211393224482No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1750.12587211393224482No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA1740.12515284470977486No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1730.1244335754873049No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC1710.12299503704236495No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA1670.12011796015248506No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1670.12011796015248506No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG1580.11364453715025534No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT1580.11364453715025534No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA1570.11292526792778537No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1570.11292526792778537No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1560.11220599870531539No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1550.11148672948284542No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1540.11076746026037546No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1520.10932892181543552No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT1480.10645184492555564No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT1470.10573257570308567No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT1460.10501330648061569No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA1450.10429403725814572No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA1430.10285549881320578No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGTTTTT251.2280504E-437.037
CGTATAC200.001836375337.03
CCGTATA200.001836375337.02
GTATACT200.001836375337.04
AAGTCCC200.001836375337.024
GGTCGGT303.5833646E-430.83333418
GCGGTCG303.5833646E-430.83333416
ACACCGC303.5833646E-430.8333346
TACTGTG303.5833646E-430.8333347
CCCTTCC303.5833646E-430.8333348
CGGTCGG303.5833646E-430.83333417
CGGTATA303.5833646E-430.83333421
AAAAGGG250.00547994129.636
CCCGTAT250.00547994129.61
TATACTG250.00547994129.65
CACCGCC250.00547994129.67
TGAGCAG250.00547994129.612
TCCGGCG358.830068E-426.4285712
GTGATCC358.830068E-426.428573
ACTGTGA358.830068E-426.428578
Produced by FastQC (version 0.11.5)