##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630237.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	139030
Sequences flagged as poor quality	0
Sequence length	43
%GC	42
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.74680284830612	31.0	31.0	34.0	30.0	34.0
2	31.85101057325757	33.0	31.0	34.0	30.0	34.0
3	31.869157735740487	33.0	31.0	34.0	30.0	34.0
4	35.6011939869093	37.0	35.0	37.0	33.0	37.0
5	35.579723800618574	37.0	35.0	37.0	33.0	37.0
6	35.70438754225707	37.0	35.0	37.0	33.0	37.0
7	35.681025677911244	37.0	35.0	37.0	33.0	37.0
8	35.73949507300583	37.0	35.0	37.0	33.0	37.0
9	37.35030568941955	39.0	37.0	39.0	34.0	39.0
10	37.291296842408116	39.0	37.0	39.0	34.0	39.0
11	37.39367762353449	39.0	37.0	39.0	34.0	39.0
12	37.290383370495576	39.0	37.0	39.0	34.0	39.0
13	37.34634970869597	39.0	37.0	39.0	34.0	39.0
14	38.350348845572896	40.0	38.0	41.0	34.0	41.0
15	38.338142846867584	40.0	38.0	41.0	34.0	41.0
16	38.17467453067683	40.0	37.0	41.0	33.0	41.0
17	38.320513558224846	40.0	38.0	41.0	34.0	41.0
18	38.43032439041934	40.0	38.0	41.0	34.0	41.0
19	38.484902539020354	40.0	38.0	41.0	34.0	41.0
20	38.53255412500899	40.0	38.0	41.0	34.0	41.0
21	38.49497230813493	40.0	38.0	41.0	34.0	41.0
22	38.44032223261166	40.0	38.0	41.0	34.0	41.0
23	38.379565561389626	40.0	38.0	41.0	34.0	41.0
24	38.329468460044595	40.0	38.0	41.0	34.0	41.0
25	38.283276990577576	40.0	37.0	41.0	34.0	41.0
26	38.020916348989424	40.0	37.0	41.0	33.0	41.0
27	37.83681939149824	40.0	37.0	41.0	33.0	41.0
28	37.70451701071711	40.0	36.0	41.0	33.0	41.0
29	37.539905056462636	39.0	36.0	41.0	33.0	41.0
30	37.3214629935985	39.0	35.0	41.0	33.0	41.0
31	37.037740056103	39.0	35.0	40.0	32.0	41.0
32	36.807386894914764	38.0	35.0	40.0	31.0	41.0
33	36.64587499100914	38.0	35.0	40.0	31.0	41.0
34	36.451816154786734	38.0	35.0	40.0	31.0	41.0
35	36.24596130331583	38.0	35.0	40.0	30.0	41.0
36	36.00060418614687	38.0	35.0	40.0	30.0	41.0
37	35.78706753937999	38.0	35.0	40.0	30.0	41.0
38	35.53919297993239	38.0	35.0	40.0	28.0	41.0
39	35.242106020283394	38.0	35.0	40.0	26.0	41.0
40	34.93240307847227	38.0	34.0	40.0	24.0	41.0
41	34.572761274545066	38.0	34.0	40.0	22.0	41.0
42	34.26236783428037	38.0	33.0	40.0	21.0	41.0
43	33.079695029849674	36.0	33.0	40.0	15.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	1.0
11	2.0
12	1.0
13	0.0
14	0.0
15	0.0
16	1.0
17	5.0
18	4.0
19	17.0
20	21.0
21	51.0
22	111.0
23	206.0
24	311.0
25	551.0
26	792.0
27	1175.0
28	1713.0
29	2373.0
30	3050.0
31	3695.0
32	4643.0
33	5880.0
34	7748.0
35	10076.0
36	13792.0
37	22435.0
38	29250.0
39	31124.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.96921527727829	18.46292167158167	14.960080558152915	20.607782492987127
2	19.2382938934043	24.452995756311587	33.3604258073797	22.94828454290441
3	21.96432424656549	25.806660433000072	31.840609940300656	20.388405380133783
4	13.829389340430124	19.123930087031578	33.04178954182551	34.0048910307128
5	13.031000503488455	40.45170107171114	31.645687981011296	14.87161044378911
6	28.511112709487165	32.653384161691726	19.162770625044953	19.672732503776164
7	24.86297921311947	34.48464360210027	20.99187225778609	19.660504926994175
8	30.849456951737036	30.760267568150756	20.417895418255053	17.972380061857155
9	28.242825289505863	12.298065165791556	21.149392217507014	38.30971732719557
10	21.92188736243976	28.04214917643674	26.242537581816872	23.793425879306625
11	30.912752643314395	25.483708552111057	18.226282097389053	25.3772567071855
12	21.02783571890959	31.109113141048695	27.80047471768683	20.062576422354887
13	33.14752211752859	19.313817161763648	27.122923110120116	20.41573761058764
14	22.670646623031	23.701359418830467	29.484283967489034	24.1437099906495
15	30.069049845357114	24.787455944760122	25.29382147737898	19.849672732503777
16	20.302812342659855	25.78867870243832	30.234481766525214	23.67402718837661
17	18.676544630655254	29.711573041789542	28.90743005106812	22.70445227648709
18	18.936200819966913	22.68790908437028	33.35898726893476	25.016902826728042
19	19.433935121916132	28.201107674602603	33.957419262029774	18.407537941451483
20	21.834136517298425	22.228296051211966	36.654678846292164	19.282888585197437
21	24.479608717542973	23.272674962238366	33.11731281018485	19.130403510033805
22	23.597784650794793	24.200532259224627	31.01704668057254	21.184636409408043
23	21.94202690066892	25.285909515931813	32.064302668488814	20.707760914910452
24	20.114363806372726	26.40149607998274	33.478385959864774	20.00575415377976
25	20.390563187801195	26.489966194346543	32.93677623534489	20.18269438250737
26	22.720276199381427	25.334100553837303	31.868661439976982	20.076961806804285
27	20.472559879162773	25.854851470905558	32.56563331655038	21.106955333381286
28	19.328921815435518	27.348773645975687	33.35395238437747	19.96835215421132
29	19.824498309717328	27.10062576422355	33.27770984679566	19.797166079263466
30	18.658562900093507	29.14694670215062	34.93562540458894	17.25886499316694
31	20.114363806372726	27.445874991009134	32.41386751060922	20.02589369200892
32	17.09846795655614	28.380205710997625	33.981155146371286	20.54017118607495
33	18.55858447817018	27.451629144788896	35.187369632453425	18.8024167445875
34	17.929943177731424	28.212615982162127	32.75839746817233	21.099043371934115
35	18.660001438538444	29.408760699129683	32.44335754873049	19.48788031360138
36	17.212112493706393	29.521685967057472	32.41602531827663	20.850176220959504
37	18.168021290368984	30.052506653240307	31.972955477235125	19.806516579155577
38	18.131338560023018	27.691145795871396	32.53470473998418	21.64281090412141
39	18.76069912968424	26.76041142199525	34.112781414083294	20.366108034237214
40	18.08098971445012	27.764511256563328	33.30288426958211	20.851614759404445
41	16.594979500827158	27.777458102567792	34.03294253038913	21.594619866215925
42	16.383514349420988	27.650147450190605	34.38610371862188	21.580234481766524
43	16.015967776738833	29.3030281234266	33.6934474573833	20.98755664245127
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	7.0
1	38.0
2	69.0
3	221.5
4	374.0
5	374.0
6	583.0
7	792.0
8	781.5
9	771.0
10	959.5
11	1148.0
12	1148.0
13	2299.0
14	3450.0
15	5352.5
16	7255.0
17	6007.5
18	4760.0
19	4760.0
20	5317.5
21	5875.0
22	3915.0
23	1955.0
24	1922.0
25	1889.0
26	1889.0
27	1961.0
28	2033.0
29	2034.0
30	2035.0
31	2071.5
32	2108.0
33	2108.0
34	2448.0
35	2788.0
36	2778.5
37	2769.0
38	3279.0
39	3789.0
40	3789.0
41	4624.0
42	5459.0
43	6752.0
44	8045.0
45	10276.0
46	12507.0
47	12507.0
48	15258.5
49	18010.0
50	15580.5
51	13151.0
52	11593.5
53	10036.0
54	10036.0
55	9208.0
56	8380.0
57	7667.5
58	6955.0
59	5850.5
60	4746.0
61	4746.0
62	3938.5
63	3131.0
64	2457.0
65	1783.0
66	1452.0
67	1121.0
68	1121.0
69	995.5
70	870.0
71	687.5
72	505.0
73	364.0
74	223.0
75	223.0
76	172.5
77	122.0
78	90.0
79	58.0
80	46.0
81	34.0
82	34.0
83	25.0
84	16.0
85	11.5
86	7.0
87	5.5
88	4.0
89	4.0
90	2.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	139030.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.131554340789755
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.50170463350379	28.034956484212042
2	10.733379823337982	7.970941523412213
3	3.936153726948706	4.38466518017694
4	2.2799473113280646	3.386319499388621
5	1.485743065240973	2.758397468172337
6	0.9588563458856346	2.1362295907358124
7	0.6779792344645901	1.7622095950514278
8	0.5772508910584224	1.7147378263684097
9	0.38935378893537886	1.3011580234481768
>10	3.006353633968697	21.2047759476372
>50	0.2924996125832946	7.539379989930231
>100	0.1530295986362932	10.197799036179243
>500	0.0	0.0
>1k	0.007748334108166745	7.608429835287348
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	3580	2.5749838164424945	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3338	2.4009206646047616	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	2228	1.6025318276630944	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1432	1.0299935265769977	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	463	0.33302165000359635	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	460	0.33086384233618643	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	410	0.2949003812126879	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	385	0.2769186506509386	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	349	0.25102495864201974	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	339	0.24383226641732	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	318	0.22872761274545061	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	313	0.2251312666331008	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	307	0.22081565129828096	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	259	0.18629072861972237	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	250	0.17981730561749262	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	240	0.17262461339279292	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	238	0.17118607494785298	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	221	0.1589584981658635	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	220	0.1582392289433935	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	219	0.15751995972092353	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	218	0.15680069049845358	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	209	0.15032726749622383	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	204	0.14673092138387397	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	204	0.14673092138387397	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	201	0.14457311371646409	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	195	0.14025749838164425	No Hit
GTACATGGGCCTCTCTATGGGCATATCAATAAGGCGAGGAATC	192	0.13809969071423434	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	186	0.13378407537941453	No Hit
CTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCATA	183	0.1316262677120046	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	183	0.1316262677120046	No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTT	176	0.1265913831547148	No Hit
GGTATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAA	175	0.12587211393224482	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	175	0.12587211393224482	No Hit
GGTATCAACGCAGAGTACATGGGAACAGTGGCTGTCTCTTATA	174	0.12515284470977486	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	173	0.1244335754873049	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCC	171	0.12299503704236495	No Hit
CCACTGTTCCCATGTACTCTGCGTTGATACCCTGTCTCTTATA	167	0.12011796015248506	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	167	0.12011796015248506	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTG	158	0.11364453715025534	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATACTGTCT	158	0.11364453715025534	No Hit
CTCTATGGGCAGTCGGTGATTCCTCCGCTTATTGATATGCCCA	157	0.11292526792778537	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	157	0.11292526792778537	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	156	0.11220599870531539	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	155	0.11148672948284542	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	154	0.11076746026037546	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	152	0.10932892181543552	No Hit
GCCTTGGAGGATGGTCCCCCCATATTCAGACAGGATACCACGT	148	0.10645184492555564	No Hit
TATCAATAAGGCGAGGAATCACCGACTGCCCATAGAGCTGTCT	147	0.10573257570308567	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAGCATGT	146	0.10501330648061569	No Hit
CTCTATGGGCATATCAATAAGGCGAGGAATCACCGACTGCCCA	145	0.10429403725814572	No Hit
TATCAACGCAGAGTACATGGGCCTCTCTATGGGCATATCAATA	143	0.10285549881320578	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	7.192692224699705E-4	0.0
10	0.0	0.0	0.0	0.001438538444939941	0.0
11	0.0	0.0	0.0	0.001438538444939941	0.0
12	0.0	0.0	0.0	0.0035963461123498523	0.0
13	0.0	0.0	0.0	0.0035963461123498523	0.0
14	0.0	0.0	0.0	0.005034884557289793	0.0
15	0.0	0.0	0.0	0.007911961447169675	0.0
16	0.0	0.0	0.0	0.025174422786448967	0.0
17	0.0	0.0	0.0	0.036682730345968494	0.0
18	0.0	0.0	0.0	0.04243688412572826	0.0
19	0.0	0.0	0.0	0.05322592246277782	0.0
20	0.0	0.0	0.0	0.05682226857512767	0.0
21	0.0	0.0	0.0	0.08559303747392649	0.0
22	0.0	0.0	0.0	0.13594188304682442	0.0
23	0.0	0.0	0.0	0.20571099762641157	0.0
24	0.0	0.0	0.0	0.31144357332949724	0.0
25	0.0	0.0	0.0	0.34381068834064593	0.0
26	0.0	0.0	0.0	0.39847514924836364	0.0
27	0.0	0.0	0.0	0.4603323023807811	0.0
28	0.0	0.0	0.0	0.5193123786233187	0.0
29	0.0	0.0	0.0	0.6243256851039344	0.0
30	0.0	0.0	0.0	0.8429835287348054	0.0
31	0.0	0.0	0.0	1.4248723297130115	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AGTTTTT	25	1.2280504E-4	37.0	37
CGTATAC	20	0.0018363753	37.0	3
CCGTATA	20	0.0018363753	37.0	2
GTATACT	20	0.0018363753	37.0	4
AAGTCCC	20	0.0018363753	37.0	24
GGTCGGT	30	3.5833646E-4	30.833334	18
GCGGTCG	30	3.5833646E-4	30.833334	16
ACACCGC	30	3.5833646E-4	30.833334	6
TACTGTG	30	3.5833646E-4	30.833334	7
CCCTTCC	30	3.5833646E-4	30.833334	8
CGGTCGG	30	3.5833646E-4	30.833334	17
CGGTATA	30	3.5833646E-4	30.833334	21
AAAAGGG	25	0.005479941	29.6	36
CCCGTAT	25	0.005479941	29.6	1
TATACTG	25	0.005479941	29.6	5
CACCGCC	25	0.005479941	29.6	7
TGAGCAG	25	0.005479941	29.6	12
TCCGGCG	35	8.830068E-4	26.42857	12
GTGATCC	35	8.830068E-4	26.42857	3
ACTGTGA	35	8.830068E-4	26.42857	8
>>END_MODULE