FastQCFastQC Report
Fri 10 Feb 2017
ERR1630236.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630236.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2227235
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT63570.28542116121558797No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT47870.21493017126616634No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT39540.1775295377452312No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT25930.11642238021582815No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA21950.021.5763111
TAGACCG500.007036902518.55
GTCTTAC1701.4879333E-915.2352931
CTTATAC10650.014.76525837
GTATCAA32950.014.5417312
TAGTACT2750.013.4545464
GTTAGAC1254.8483686E-513.323
TCTATAC1409.567642E-613.2142853
GACGGAC3100.013.1290327
TTAGACC2305.7661964E-1012.8695654
GAGCGAA3450.012.86956516
ACGGACC3050.012.7377058
CGATCGC1901.047174E-712.65789519
CGGACCA3400.012.5147069
CGCAAGA3550.012.5070432
TCTGTCG1807.4707896E-712.3333348
GTCTAAT1050.002644911212.3333331
GCGCAAG4050.012.3333331
AAGACGG3350.012.1492545
TACACTC2603.092282E-1012.0961555
Produced by FastQC (version 0.11.5)