FastQCFastQC Report
Fri 10 Feb 2017
ERR1630235.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630235.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1752080
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT59920.34199351627779556No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT54380.3103739555271449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43610.2489041596274143No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26830.1531322770649742No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT22030.12573626775033103No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA20460.1167754897036665No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA19890.11352221359755262No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC19770.11283731336468655No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18300.023.7568321
GACGGAC2200.019.3409087
AAGACCG609.239454E-418.55
CGGACCA2300.018.59
CGCGCTT752.0680454E-417.26666612
ATCGCTA752.0680454E-417.26666612
CGCCGTA650.001580381217.07692323
AAGACGG2750.016.8181825
GTATCAA28000.015.3946432
TATACTG2055.456968E-1215.3414635
TTAGTAC855.366145E-415.2352943
TCGCGGA855.366145E-415.23529432
GCTTTAT7200.014.9027791
CGTATAA750.004105547414.830
GTTCGCG750.004105547414.830
CGAAAGC3150.014.09523919
ACGGACC3050.013.950828
AGACGGA3450.013.9420286
CGCGGAA800.006300360413.87533
TAACGCC1601.7847924E-713.8754
Produced by FastQC (version 0.11.5)