##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630235.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1752080
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.75610303182503	31.0	31.0	34.0	30.0	34.0
2	31.971023012647823	33.0	31.0	34.0	30.0	34.0
3	32.09541288069038	34.0	31.0	34.0	30.0	34.0
4	35.749951486233506	37.0	35.0	37.0	33.0	37.0
5	35.67922355143601	37.0	35.0	37.0	33.0	37.0
6	35.73710104561435	37.0	35.0	37.0	33.0	37.0
7	35.72165711611342	37.0	35.0	37.0	33.0	37.0
8	35.72485902470207	37.0	35.0	37.0	33.0	37.0
9	37.445375781927766	39.0	37.0	39.0	35.0	39.0
10	37.33530946075522	39.0	37.0	39.0	34.0	39.0
11	37.40802360622803	39.0	37.0	39.0	34.0	39.0
12	37.33927731610429	39.0	37.0	39.0	34.0	39.0
13	37.34968665814346	39.0	37.0	39.0	34.0	39.0
14	38.55981690333775	40.0	38.0	41.0	34.0	41.0
15	38.565864572393956	40.0	38.0	41.0	34.0	41.0
16	38.51304449568513	40.0	38.0	41.0	34.0	41.0
17	38.49699842472946	40.0	38.0	41.0	34.0	41.0
18	38.45592952376604	40.0	38.0	41.0	34.0	41.0
19	38.48192148760331	40.0	38.0	41.0	34.0	41.0
20	38.47346639422857	40.0	38.0	41.0	34.0	41.0
21	38.41413748230674	40.0	38.0	41.0	34.0	41.0
22	38.37521802657413	40.0	38.0	41.0	34.0	41.0
23	38.324276288753936	40.0	38.0	41.0	34.0	41.0
24	38.28143977443953	40.0	38.0	41.0	34.0	41.0
25	38.266067188712846	40.0	38.0	41.0	34.0	41.0
26	38.09818158988174	40.0	38.0	41.0	33.0	41.0
27	37.97678359435642	40.0	37.0	41.0	33.0	41.0
28	37.902885712981146	40.0	37.0	41.0	33.0	41.0
29	37.83893201223688	40.0	37.0	41.0	33.0	41.0
30	37.779618510570295	40.0	37.0	41.0	33.0	41.0
31	37.718079653897085	40.0	37.0	41.0	33.0	41.0
32	37.642667572256975	40.0	37.0	41.0	32.0	41.0
33	37.53525980548834	40.0	37.0	41.0	32.0	41.0
34	37.48066983242774	40.0	37.0	41.0	32.0	41.0
35	37.34918896397425	40.0	36.0	41.0	31.0	41.0
36	37.26362951463403	39.0	36.0	41.0	31.0	41.0
37	37.20512362449203	39.0	36.0	41.0	31.0	41.0
38	37.09845840372586	39.0	36.0	41.0	31.0	41.0
39	37.00677080955207	39.0	36.0	41.0	31.0	41.0
40	36.89671932788457	39.0	36.0	41.0	30.0	41.0
41	36.78055111638738	39.0	35.0	41.0	30.0	41.0
42	36.7456891237843	39.0	35.0	41.0	30.0	41.0
43	35.53171544678325	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	7.0
11	3.0
12	0.0
13	1.0
14	3.0
15	9.0
16	9.0
17	26.0
18	65.0
19	113.0
20	266.0
21	547.0
22	1000.0
23	1797.0
24	2884.0
25	4660.0
26	6837.0
27	10499.0
28	14896.0
29	20801.0
30	28335.0
31	36837.0
32	47781.0
33	61918.0
34	81554.0
35	108152.0
36	152015.0
37	231186.0
38	423724.0
39	516151.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	42.60416191041505	19.629411899000047	12.531162960595408	25.235263229989496
2	19.09775809323775	21.35581708597781	33.53454180174421	26.011883019040226
3	20.25786493767408	23.049404136797406	29.90651111821378	26.786219807314733
4	14.74835623944112	15.702079813707137	35.16523218117894	34.3843317656728
5	14.575590155700652	36.43458061275741	34.41087164969636	14.578957581845579
6	34.19067622482992	35.39775581023697	15.64169444317611	14.769873521756999
7	29.033834071503584	30.787977717912423	21.377505593351902	18.80068261723209
8	27.873784302086662	32.372437331628696	20.243539107803297	19.51023925848135
9	26.734509839733345	14.249748869914617	20.43108762156979	38.584653668782245
10	17.480023743208072	26.613682023651887	33.278731564768734	22.627562668371308
11	34.82654901602666	22.258116067759463	21.55597917903292	21.35935573718095
12	21.898600520524177	25.93534541801744	28.91939865759554	23.24665540386284
13	30.444842701246515	20.00570750194055	24.8797429341126	24.669706862700334
14	22.801698552577506	21.359298662161542	25.516528925619834	30.322473859641114
15	25.35689009634263	28.121432811287157	22.46227341217296	24.05940368019725
16	24.398885895621206	26.702205378749827	24.470743345052735	24.42816538057623
17	23.475925756814757	26.41157937993699	25.91502671110908	24.19746815213917
18	23.106593306241724	25.286402447376833	27.314163736815672	24.292840509565774
19	24.11773435002968	25.358659421944203	27.51854938130679	23.005056846719327
20	24.966668188667185	25.10199305967764	26.384925345874617	23.54641340578056
21	24.75817314277887	25.180984886534862	26.309300945162324	23.75154102552395
22	24.73237523400758	25.261517738916034	26.80088808730195	23.20521893977444
23	24.26139217387334	25.158725628966717	26.300454317154472	24.279427880005482
24	23.698917857632072	25.650541071183962	26.992774302543264	23.657766768640702
25	24.343237751700837	25.582964248207844	26.279165334916215	23.794632665175104
26	23.871912241450165	26.024953198484084	26.365919364412584	23.737215195653167
27	24.162595315282406	25.120713666042647	26.533719921464773	24.182971097210174
28	23.738128395963653	25.556652664261907	26.633601205424412	24.071617734350028
29	23.511141043787955	25.91028948449842	27.054586548559428	23.523982923154193
30	23.947707867220675	26.142984338614678	26.173804849093653	23.735502945071
31	23.550237432080724	26.443997990959318	26.600155244052782	23.405609332907172
32	23.391112277978173	25.70510478973563	26.601867494634952	24.301915437651246
33	22.80358202821789	26.097895073284327	27.081354732660607	24.017168165837177
34	24.482957399205514	24.79972375690608	26.763789324688368	23.953529519200035
35	23.507602392584815	25.21756997397379	27.207433450527375	24.067394182914022
36	23.13193461485777	26.023069722843704	27.037235742660155	23.80775991963837
37	23.81860417332542	24.885222135975525	27.435162777955348	23.861010912743712
38	23.631055659558925	25.480971188530205	27.0356376421168	23.852335509794074
39	23.328386831651525	25.027224784256425	27.69319894068764	23.95118944340441
40	22.6585544039085	24.65880553399388	28.34413953700744	24.33850052509018
41	22.177925665494726	24.880142459248436	28.57734806629834	24.364583808958496
42	22.44104150495411	24.759600018264006	28.761072553764667	24.038285923017213
43	22.608442536870463	24.414467375918907	28.68967170448838	24.28741838272225
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	890.0
1	681.5
2	473.0
3	856.0
4	1239.0
5	1239.0
6	1598.0
7	1957.0
8	1747.0
9	1537.0
10	2211.0
11	2885.0
12	2885.0
13	5159.5
14	7434.0
15	10271.5
16	13109.0
17	13157.0
18	13205.0
19	13205.0
20	16716.0
21	20227.0
22	20662.0
23	21097.0
24	25207.0
25	29317.0
26	29317.0
27	33214.5
28	37112.0
29	42050.0
30	46988.0
31	53067.0
32	59146.0
33	59146.0
34	64721.5
35	70297.0
36	76118.0
37	81939.0
38	84680.5
39	87422.0
40	87422.0
41	90694.0
42	93966.0
43	95419.0
44	96872.0
45	99601.0
46	102330.0
47	102330.0
48	107390.5
49	112451.0
50	112565.0
51	112679.0
52	116536.0
53	120393.0
54	120393.0
55	116781.0
56	113169.0
57	111205.5
58	109242.0
59	105479.5
60	101717.0
61	101717.0
62	94733.5
63	87750.0
64	79575.0
65	71400.0
66	61096.5
67	50793.0
68	50793.0
69	43963.0
70	37133.0
71	30776.0
72	24419.0
73	17364.5
74	10310.0
75	10310.0
76	7537.5
77	4765.0
78	3771.0
79	2777.0
80	2204.5
81	1632.0
82	1632.0
83	1192.0
84	752.0
85	636.0
86	520.0
87	455.5
88	391.0
89	391.0
90	329.5
91	268.0
92	162.5
93	57.0
94	34.5
95	12.0
96	12.0
97	8.0
98	4.0
99	4.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1752080.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	53.46489405573549
#Duplication Level	Percentage of deduplicated	Percentage of total
1	78.66887770305648	42.06023211877527
2	12.839339870181155	13.729078918096322
3	3.956048009926879	6.345290631904316
4	1.5162144317594881	3.2425697583919293
5	0.7668619996156032	2.050009778240885
6	0.4258003535221709	1.3659222473974553
7	0.2887074375306373	1.0804998792474878
8	0.19992946472164905	0.8551366119970298
9	0.1503217382224463	0.7233242227503386
>10	0.9761980109594517	10.362131092244327
>50	0.11377714185193938	4.232108402846701
>100	0.0891389438759144	9.308412277665575
>500	0.007392167555631952	2.763228035066167
>1k	0.0011784614943761082	1.2273630930599442
>5k	2.142657262502015E-4	0.6546929323163694
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	5992	0.34199351627779556	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5438	0.3103739555271449	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4361	0.2489041596274143	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2683	0.1531322770649742	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	2203	0.12573626775033103	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	2046	0.1167754897036665	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	1989	0.11352221359755262	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1977	0.11283731336468655	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	5.70750194055066E-5	0.0
11	0.0	0.0	0.0	1.141500388110132E-4	0.0
12	0.0	0.0	0.0	1.712250582165198E-4	0.0
13	0.0	0.0	0.0	1.712250582165198E-4	0.0
14	0.0	0.0	0.0	3.424501164330396E-4	0.0
15	0.0	0.0	0.0	3.424501164330396E-4	0.0
16	0.0	0.0	0.0	3.9952513583854616E-4	0.0
17	0.0	0.0	0.0	4.566001552440528E-4	0.0
18	0.0	0.0	0.0	4.566001552440528E-4	0.0
19	0.0	0.0	0.0	5.707501940550659E-4	0.0
20	0.0	0.0	0.0	6.849002328660792E-4	0.0
21	0.0	0.0	0.0	9.702753298936121E-4	0.0
22	0.0	0.0	0.0	0.0012556504269211452	0.0
23	0.0	0.0	0.0	0.0022259257568147573	0.0
24	0.0	0.0	0.0	0.003367426144924889	0.0
25	0.0	0.0	0.0	0.0045660015524405274	0.0
26	0.0	0.0	0.0	0.007476827542121364	0.0
27	0.0	0.0	0.0	0.020204556869549337	0.0
28	0.0	0.0	0.0	0.05422126843523127	0.0
29	0.0	0.0	0.0	0.10182183461942378	0.0
30	0.0	0.0	0.0	0.1667161316834848	0.0
31	0.0	0.0	0.0	0.3253276106113876	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTATCA	1830	0.0	23.756832	1
GACGGAC	220	0.0	19.340908	7
AAGACCG	60	9.239454E-4	18.5	5
CGGACCA	230	0.0	18.5	9
CGCGCTT	75	2.0680454E-4	17.266666	12
ATCGCTA	75	2.0680454E-4	17.266666	12
CGCCGTA	65	0.0015803812	17.076923	23
AAGACGG	275	0.0	16.818182	5
GTATCAA	2800	0.0	15.394643	2
TATACTG	205	5.456968E-12	15.341463	5
TTAGTAC	85	5.366145E-4	15.235294	3
TCGCGGA	85	5.366145E-4	15.235294	32
GCTTTAT	720	0.0	14.902779	1
CGTATAA	75	0.0041055474	14.8	30
GTTCGCG	75	0.0041055474	14.8	30
CGAAAGC	315	0.0	14.095239	19
ACGGACC	305	0.0	13.95082	8
AGACGGA	345	0.0	13.942028	6
CGCGGAA	80	0.0063003604	13.875	33
TAACGCC	160	1.7847924E-7	13.875	4
>>END_MODULE