FastQCFastQC Report
Fri 10 Feb 2017
ERR1630234.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630234.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1164693
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT64250.5516475156972696No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT60910.5229704308345633No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT45770.39297909406169695No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29490.25319977023988294No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGGTA802.7259375E-823.12523
GTCGTAC400.001931202723.1251
TCTATAC1601.8189894E-1218.53
CGCCTTA500.007034938318.49999825
GCTATAC1351.1514203E-917.8148163
CGGGTAA953.6064703E-617.52631624
CCCGTTG852.7231654E-517.41176431
TCGTATT852.7231654E-517.41176426
TACGAAT752.0672954E-417.2666667
CTTATAC5450.016.97247737
GCATTCG1005.877926E-616.6522
ACCCGTT1005.877926E-616.6530
CGTATTG904.4460394E-516.44444527
GGTATCA25200.016.2976191
TATAGAC700.002592521515.8571422
TACGTTA700.002592521515.85714219
TAACCCG957.0598035E-515.57894828
CGCGGGT1201.9354557E-615.41666722
TACGCCG1201.9354557E-615.41666710
TATACAG1954.1836756E-1115.1794875
Produced by FastQC (version 0.11.5)