FastQCFastQC Report
Fri 10 Feb 2017
ERR1630233.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630233.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1090391
Sequences flagged as poor quality0
Sequence length43
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT127241.1669208568302563No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT74530.6835162799399481No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT71610.6567368952971916No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT39100.35858696559307623No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA19170.17580849438412458No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA13110.12023210022826675No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA12830.11766421402964625No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT11180.1025320275020612No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGATTA450.003825683420.55555719
CGCGGGT1500.019.73333422
ACCCGTT1500.019.73333430
ACGGACC2100.019.3809538
CGTGAAT500.00703465518.516
GTTAGAC801.616546E-518.53
GCGCAAG2300.018.51
TAAGCGG500.00703465518.55
CGTTTGA500.00703465518.526
GCGCGGG2250.018.08888821
GCGGGTA1653.6379788E-1217.93939423
AACGCCG1451.546141E-1017.8620685
AATAACG1451.546141E-1017.8620682
CCGTTGA1800.017.47222332
CCCGTTG1705.456968E-1217.41176431
CGCATCG1401.8681021E-917.17857213
TTAGCGG650.001579863417.07692327
TAGCGGG650.001579863417.07692328
CGGTCGA650.001579863417.07692316
TGTACGT650.001579863417.07692312
Produced by FastQC (version 0.11.5)