##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630233.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1090391
Sequences flagged as poor quality	0
Sequence length	43
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537656675449448	31.0	31.0	34.0	30.0	34.0
2	31.72756011375736	31.0	31.0	34.0	30.0	34.0
3	31.84210251185125	33.0	31.0	34.0	30.0	34.0
4	35.55796406976947	37.0	35.0	37.0	33.0	37.0
5	35.48618064529146	37.0	35.0	37.0	33.0	37.0
6	35.55465975049317	37.0	35.0	37.0	33.0	37.0
7	35.527101746070905	37.0	35.0	37.0	33.0	37.0
8	35.5461041039407	37.0	35.0	37.0	33.0	37.0
9	37.22202219203937	39.0	37.0	39.0	34.0	39.0
10	37.06669350719146	39.0	37.0	39.0	33.0	39.0
11	37.171894302135655	39.0	37.0	39.0	33.0	39.0
12	37.07993096054535	39.0	37.0	39.0	33.0	39.0
13	37.11790999742294	39.0	37.0	39.0	33.0	39.0
14	38.209333165809326	40.0	38.0	41.0	33.0	41.0
15	38.20234759824687	40.0	38.0	41.0	33.0	41.0
16	38.15477658931521	40.0	37.0	41.0	33.0	41.0
17	38.16928698054184	40.0	38.0	41.0	33.0	41.0
18	38.14582016909531	40.0	38.0	41.0	33.0	41.0
19	38.19447610994588	40.0	38.0	41.0	33.0	41.0
20	38.180620529699894	40.0	38.0	41.0	33.0	41.0
21	38.12377211477351	40.0	37.0	41.0	33.0	41.0
22	38.08114887228526	40.0	37.0	41.0	33.0	41.0
23	38.015513701048526	40.0	37.0	41.0	33.0	41.0
24	37.96852596912483	40.0	37.0	41.0	33.0	41.0
25	37.94748030752272	40.0	37.0	41.0	33.0	41.0
26	37.7446310543649	40.0	37.0	41.0	33.0	41.0
27	37.597231635257444	40.0	37.0	41.0	32.0	41.0
28	37.50666504033874	39.0	37.0	41.0	32.0	41.0
29	37.40350663202466	39.0	36.0	41.0	32.0	41.0
30	37.27580656846948	39.0	36.0	41.0	32.0	41.0
31	37.167517890371435	39.0	36.0	40.0	31.0	41.0
32	37.038676951662296	39.0	35.0	40.0	31.0	41.0
33	36.91109060878162	39.0	35.0	40.0	31.0	41.0
34	36.82077988538057	39.0	35.0	40.0	31.0	41.0
35	36.65888841709075	39.0	35.0	40.0	30.0	41.0
36	36.51827280305872	39.0	35.0	40.0	30.0	41.0
37	36.41224111350883	39.0	35.0	40.0	30.0	41.0
38	36.263787943957716	39.0	35.0	40.0	30.0	41.0
39	36.14184177969187	38.0	35.0	40.0	30.0	41.0
40	35.96317651191178	38.0	35.0	40.0	29.0	41.0
41	35.786638004165475	38.0	35.0	40.0	28.0	41.0
42	35.66784483731065	38.0	35.0	40.0	27.0	41.0
43	34.454725873562786	38.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	4.0
10	10.0
11	13.0
12	4.0
13	8.0
14	8.0
15	4.0
16	10.0
17	20.0
18	75.0
19	130.0
20	248.0
21	499.0
22	971.0
23	1554.0
24	2504.0
25	3997.0
26	6100.0
27	8696.0
28	12276.0
29	16721.0
30	21784.0
31	27962.0
32	35420.0
33	45231.0
34	58632.0
35	76867.0
36	108188.0
37	164748.0
38	268349.0
39	229358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.16589186814638	19.807573613501948	13.074576000719007	23.951958517632665
2	17.429894414022126	23.302375019603062	34.67508444218633	24.59264612418848
3	19.267858960684745	25.20976420384981	31.13552844805212	24.386848387413323
4	13.472506651283805	16.371466749083584	35.83686952661935	34.31915707301326
5	13.192056794305895	38.94914759934739	34.05704926031121	13.801746346035504
6	32.267232579872726	37.0441428808565	16.6146822561815	14.073942283089277
7	27.728218593146863	32.48706198051892	21.82226375676248	17.962455669571742
8	28.666322447635757	33.43305291404643	20.15304601743778	17.747578620880034
9	27.04451889276416	13.80229660736378	20.58784417699706	38.56534032287501
10	16.637793231969084	28.500785498046117	32.66131140113959	22.200109868845214
11	34.61794897426703	23.375651486485125	21.556028984098365	20.450370555149483
12	20.675152307750157	27.707858924000657	30.184676872791506	21.432311895457683
13	30.080218930640477	20.184502623370882	25.85384508859666	23.88143335739198
14	21.975786667351436	21.39195939805079	27.373666877294472	29.2585870573033
15	24.199759535799544	28.91724161332953	23.824114469029915	23.05888438184101
16	22.887111137197575	27.490505699331706	25.580640339107713	24.041742824363006
17	21.598123975711463	27.923744785127536	27.616882384392387	22.861248854768608
18	21.724684081214903	26.175014283866982	29.135695360655028	22.964606274263087
19	22.240278945809347	26.702898318126252	29.588560433826032	21.468262302238372
20	22.71387052901207	26.27763802159042	29.253909836012955	21.75458161338456
21	23.586768416100277	26.209955878212494	28.552601773125417	21.650673932561805
22	23.31145433151961	26.353207244006967	28.445300814111636	21.890037610361787
23	22.40554076473485	26.587801990295223	28.544072722537145	22.46258452243278
24	22.10161309108384	27.157781016167593	28.544347853201284	22.19625803954728
25	22.269351085986585	26.664104894482804	28.674576367559894	22.391967651970717
26	22.133803378787974	27.222528432461385	28.676960833315757	21.966707355434885
27	22.185619653867285	27.040667063466223	28.49482433365646	22.278888949010035
28	21.907187421759716	27.196024178482762	29.12404816253986	21.772740237217658
29	21.738715745085937	27.301032381962067	29.177331801161234	21.782920071790763
30	21.515768196912852	28.058100259448217	28.819661937781955	21.606469605856983
31	21.916266733676267	27.776916720699273	28.433837036439225	21.872979509185235
32	21.248066060706662	27.747569449857895	28.640368454985417	22.36399603445003
33	21.186436791939773	27.57267805768756	29.12863367360882	22.112251476763838
34	21.822080336319726	27.315155756054484	28.86368284404402	21.99908106358178
35	21.808965774662482	27.57231121680205	29.069755711483314	21.548967297052158
36	21.389116381188032	27.910630223470296	28.434570718210257	22.265682677131416
37	21.554928461441815	27.407691369426196	28.78325297989437	22.25412718923762
38	21.342802719391486	27.326252692841376	28.979421143424698	22.35152344434244
39	21.294471432724592	26.997196418532432	29.341584807651564	22.366747341091404
40	21.201935819352872	26.813959396216585	29.910463310867385	22.073641473563153
41	20.656810263474295	26.7620514109159	30.11277605923013	22.468362266379675
42	20.714312572279116	26.931348479582095	30.013453889476345	22.340885058662444
43	20.476691388685342	26.137596513544224	30.271709872880464	23.11400222488997
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	348.0
1	304.0
2	260.0
3	758.5
4	1257.0
5	1257.0
6	1867.5
7	2478.0
8	2351.5
9	2225.0
10	2977.5
11	3730.0
12	3730.0
13	7104.0
14	10478.0
15	17964.5
16	25451.0
17	21593.5
18	17736.0
19	17736.0
20	19383.5
21	21031.0
22	17974.5
23	14918.0
24	17178.5
25	19439.0
26	19439.0
27	22052.0
28	24665.0
29	27979.0
30	31293.0
31	34999.5
32	38706.0
33	38706.0
34	42834.0
35	46962.0
36	51290.5
37	55619.0
38	58225.5
39	60832.0
40	60832.0
41	63845.5
42	66859.0
43	67493.0
44	68127.0
45	69869.5
46	71612.0
47	71612.0
48	72996.5
49	74381.0
50	71388.5
51	68396.0
52	67582.0
53	66768.0
54	66768.0
55	64641.0
56	62514.0
57	59463.0
58	56412.0
59	52791.5
60	49171.0
61	49171.0
62	44437.5
63	39704.0
64	34289.0
65	28874.0
66	24418.0
67	19962.0
68	19962.0
69	16873.0
70	13784.0
71	11793.0
72	9802.0
73	8248.0
74	6694.0
75	6694.0
76	5519.5
77	4345.0
78	3383.0
79	2421.0
80	1888.5
81	1356.0
82	1356.0
83	1006.5
84	657.0
85	544.0
86	431.0
87	395.5
88	360.0
89	360.0
90	315.0
91	270.0
92	157.5
93	45.0
94	29.5
95	14.0
96	14.0
97	8.5
98	3.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1090391.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	63.18428849132478
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.95170440631152	52.41244422055483
2	10.914134398005496	13.79203632873341
3	2.8338356960879705	5.371616764759095
4	1.0792724727963052	2.727722531276289
5	0.5641778173028849	1.7823586984435698
6	0.30771584734119173	1.1665684123054987
7	0.21720095458908212	0.9606581442743387
8	0.16422389936617984	0.8301096187778396
9	0.11953379104916265	0.6797391784300825
>10	0.7329861113846459	8.919270517875722
>50	0.07556067126488435	3.3143456931042987
>100	0.037321351758981214	4.107185955049393
>500	0.0010205049494582932	0.4416871720637736
>1k	8.747185281071085E-4	0.9760411418535444
>5k	2.915728427023695E-4	1.3461556480792467
>10k+	1.4578642135118475E-4	1.1720599744190732
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12724	1.1669208568302563	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7453	0.6835162799399481	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	7161	0.6567368952971916	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3910	0.35858696559307623	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	1917	0.17580849438412458	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	1311	0.12023210022826675	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	1283	0.11766421402964625	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1118	0.1025320275020612	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	9.171022137930339E-5	0.0
2	0.0	0.0	0.0	9.171022137930339E-5	0.0
3	0.0	0.0	0.0	9.171022137930339E-5	0.0
4	0.0	0.0	0.0	1.8342044275860677E-4	0.0
5	6.419715496551237E-4	0.0	0.0	1.8342044275860677E-4	0.0
6	7.336817710344271E-4	0.0	0.0	1.8342044275860677E-4	0.0
7	7.336817710344271E-4	0.0	0.0	1.8342044275860677E-4	0.0
8	7.336817710344271E-4	0.0	0.0	1.8342044275860677E-4	0.0
9	7.336817710344271E-4	0.0	0.0	1.8342044275860677E-4	0.0
10	7.336817710344271E-4	0.0	0.0	2.7513066413791014E-4	0.0
11	0.0011005226565516406	0.0	0.0	5.502613282758203E-4	0.0
12	0.0011005226565516406	0.0	0.0	9.171022137930339E-4	0.0
13	0.0011005226565516406	0.0	0.0	9.171022137930339E-4	0.0
14	0.0011005226565516406	0.0	0.0	0.001192232877930944	0.0
15	0.001192232877930944	0.0	0.0	0.0015590737634481576	0.0
16	0.001192232877930944	0.0	0.0	0.0022927555344825845	0.0
17	0.001650783984827461	0.0	0.0	0.0032098577482756185	0.0
18	0.001650783984827461	0.0	0.0	0.003301567969654922	0.0
19	0.0018342044275860678	0.0	0.0	0.004402090626206562	0.0
20	0.0018342044275860678	0.0	0.0	0.005502613282758203	0.0
21	0.0019259146489653712	0.0	0.0	0.007245107488964967	0.0
22	0.0019259146489653712	0.0	0.0	0.010179834573102677	0.0
23	0.0019259146489653712	0.0	0.0	0.013573112764136901	0.0
24	0.0020176248703446743	0.0	0.0	0.018250334054481374	0.0
25	0.0020176248703446743	0.0	0.0	0.020543089588963958	0.0
26	0.0020176248703446743	0.0	0.0	0.025312021100687736	0.0
27	0.0021093350917239777	0.0	0.0	0.04117788939930722	0.0
28	0.0022927555344825845	0.0	0.0	0.08382314234068329	0.0
29	0.0024761759772411913	0.0	0.0	0.14380162712274772	0.0
30	0.002659596419999798	0.0	0.0	0.23422790540274085	0.0
31	0.0027513066413791015	0.0	0.0	0.43195514269651897	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGATTA	45	0.0038256834	20.555557	19
CGCGGGT	150	0.0	19.733334	22
ACCCGTT	150	0.0	19.733334	30
ACGGACC	210	0.0	19.380953	8
CGTGAAT	50	0.007034655	18.5	16
GTTAGAC	80	1.616546E-5	18.5	3
GCGCAAG	230	0.0	18.5	1
TAAGCGG	50	0.007034655	18.5	5
CGTTTGA	50	0.007034655	18.5	26
GCGCGGG	225	0.0	18.088888	21
GCGGGTA	165	3.6379788E-12	17.939394	23
AACGCCG	145	1.546141E-10	17.862068	5
AATAACG	145	1.546141E-10	17.862068	2
CCGTTGA	180	0.0	17.472223	32
CCCGTTG	170	5.456968E-12	17.411764	31
CGCATCG	140	1.8681021E-9	17.178572	13
TTAGCGG	65	0.0015798634	17.076923	27
TAGCGGG	65	0.0015798634	17.076923	28
CGGTCGA	65	0.0015798634	17.076923	16
TGTACGT	65	0.0015798634	17.076923	12
>>END_MODULE