FastQCFastQC Report
Fri 10 Feb 2017
ERR1630232.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630232.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1194326
Sequences flagged as poor quality0
Sequence length43
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT58300.4881414287221412No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT45550.3813866565745031No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT38000.31817108561649No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19150.16034148130409956No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC12310.10307068589313136No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTATCG450.00382589420.5555555
TGTACGT450.00382589420.55555512
AAGACGG3250.019.9230775
GACGGAC3250.019.9230777
CGGACCA3400.019.0441179
GGTATCA17950.018.8607251
CGCAAGA3450.018.7681162
CGTTAAC609.2371047E-418.515
TTAGTCG500.00703503818.55
CGGTACT609.2371047E-418.54
TATCGCG500.00703503818.57
GCGTACA500.00703503818.52
TACACGG609.2371047E-418.55
AAGTCGC609.2371047E-418.529
ACGGACC3700.018.08
AGACGGA3600.017.4722216
CGTTATT1705.456968E-1217.4117642
CAAGACG3900.017.0769234
GCGAAAG3700.017.018
GCGCAAG3850.016.8181821
Produced by FastQC (version 0.11.5)