##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630232.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	1194326
Sequences flagged as poor quality	0
Sequence length	43
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.135715876569712	31.0	31.0	34.0	28.0	34.0
2	31.363362264574327	31.0	31.0	34.0	28.0	34.0
3	31.476959389647384	31.0	31.0	34.0	28.0	34.0
4	35.26702424631131	37.0	35.0	37.0	32.0	37.0
5	35.0402737611004	37.0	35.0	37.0	32.0	37.0
6	35.115524572018025	36.0	35.0	37.0	32.0	37.0
7	35.10708885178754	36.0	35.0	37.0	32.0	37.0
8	35.1082518508347	37.0	35.0	37.0	32.0	37.0
9	36.74293953242247	38.0	37.0	39.0	33.0	39.0
10	36.5132183340227	38.0	35.0	39.0	32.0	39.0
11	36.645035777501285	38.0	35.0	39.0	32.0	39.0
12	36.52753352099845	38.0	35.0	39.0	32.0	39.0
13	36.57384164792527	38.0	35.0	39.0	32.0	39.0
14	37.58094104959618	39.0	37.0	40.0	32.0	41.0
15	37.55877122326735	39.0	37.0	40.0	32.0	41.0
16	37.495493692676874	39.0	36.0	40.0	32.0	41.0
17	37.46272876919702	39.0	36.0	40.0	32.0	41.0
18	37.409780914088785	39.0	36.0	40.0	32.0	41.0
19	37.43621004650321	39.0	36.0	40.0	32.0	41.0
20	37.420655666878226	39.0	36.0	40.0	32.0	41.0
21	37.337494118021375	39.0	36.0	40.0	32.0	41.0
22	37.27824312624861	39.0	36.0	40.0	32.0	41.0
23	37.209904163519845	39.0	36.0	40.0	32.0	41.0
24	37.14623226824167	39.0	36.0	40.0	31.0	41.0
25	37.132913459139296	39.0	36.0	40.0	31.0	41.0
26	36.914073711867616	39.0	36.0	40.0	31.0	41.0
27	36.76740270244473	39.0	36.0	40.0	31.0	41.0
28	36.65820387398416	39.0	36.0	40.0	30.0	41.0
29	36.57454246160596	38.0	35.0	40.0	30.0	41.0
30	36.48554582249737	38.0	35.0	40.0	30.0	41.0
31	36.40372142949245	38.0	35.0	40.0	30.0	41.0
32	36.29484579587148	38.0	35.0	40.0	30.0	41.0
33	36.168990711078884	38.0	35.0	40.0	30.0	41.0
34	36.09084454328215	38.0	35.0	40.0	30.0	41.0
35	35.92152142714803	38.0	35.0	40.0	29.0	41.0
36	35.80044644427066	38.0	35.0	40.0	28.0	41.0
37	35.728467771780906	38.0	34.0	40.0	28.0	41.0
38	35.58440325338308	38.0	34.0	40.0	27.0	41.0
39	35.476270298059326	38.0	34.0	40.0	27.0	41.0
40	35.32488700739999	38.0	34.0	40.0	27.0	41.0
41	35.19882594869407	38.0	34.0	40.0	26.0	41.0
42	35.12082128330121	38.0	34.0	40.0	26.0	41.0
43	33.82717281546245	37.0	32.0	39.0	23.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	1.0
9	4.0
10	5.0
11	6.0
12	6.0
13	5.0
14	6.0
15	18.0
16	29.0
17	65.0
18	133.0
19	276.0
20	489.0
21	858.0
22	1519.0
23	2547.0
24	3982.0
25	6186.0
26	8909.0
27	12509.0
28	16937.0
29	22782.0
30	29364.0
31	37525.0
32	48211.0
33	60796.0
34	79571.0
35	106354.0
36	149729.0
37	216279.0
38	276295.0
39	112929.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.941396235198766	20.34896669753484	13.024500848177132	24.68513621908926
2	18.641895093969318	22.02514221410235	33.906655301818766	25.42630739010957
3	20.09660678910113	23.7259341251886	30.19527331733547	25.982185768374798
4	14.586972066253267	16.169538300263078	35.3856484745371	33.85784115894655
5	14.179126972032762	37.38158593214918	34.26660727473069	14.172679821087375
6	33.8427698969963	36.48476211687596	15.632498999435665	14.039968986692076
7	28.954741000363388	31.326957631333492	21.344758466281398	18.373542902021725
8	28.123225986874605	33.246534028397605	19.977795007393294	18.652444977334497
9	26.801560043070317	14.015771238338612	19.995629334034426	39.18703938455664
10	16.981544402449583	27.15833030512607	32.78275780649504	23.077367485929305
11	36.06352034536634	21.991566791646502	21.3513730756929	20.593539787294258
12	21.994413585570438	26.14587641900118	29.132330703677223	22.727379291751163
13	30.462620758486374	19.491997997196744	25.217235495166314	24.82814574915057
14	22.579178549240325	20.73629812965639	25.703534880761197	30.980988440342085
15	25.056224179997756	28.283818655877873	22.44688636101031	24.21307080311406
16	24.496829173944132	26.667007165547762	24.258200859731765	24.577962800776337
17	23.298496390432764	26.66550003935274	25.81430865609557	24.221694914118927
18	23.47625355221271	25.129570988155663	27.303014419848516	24.091161039783106
19	24.18334692537883	25.34601105560793	27.407257315004447	23.063384704008786
20	24.98120278717871	24.81784705348456	26.522239321592263	23.678710837744468
21	25.362673172986273	24.92426690869997	26.216125245535977	23.496934672777783
22	24.88667248305739	25.097000316496498	26.72771085951407	23.288616340932037
23	23.968497713354644	25.02976574235175	26.449059971900468	24.552676572393132
24	23.66380703426033	25.58262986822693	27.000416971580627	23.753146125932115
25	24.149604044456872	25.30364406368111	26.467731590872173	24.079020300989846
26	23.91750661042295	25.96937519571708	26.51168943822708	23.601428755632885
27	24.178323171395412	25.045004462768123	26.655117614453676	24.12155475138279
28	23.764282113928694	25.568228440141134	26.8950018671619	23.772487578768274
29	23.516192396380887	25.767001639418385	27.128355239691675	23.588450724509052
30	23.665565348154523	26.106942325629685	26.48573337597942	23.741758950236367
31	23.80246264420267	26.290225616791396	26.449980993464095	23.45733074554184
32	23.27672678983795	25.61587037375055	26.53471497731775	24.572687859093747
33	22.883031936004073	25.880789667142807	27.016241796628393	24.219936600224727
34	24.418039965637522	24.96345218977063	26.77292464536483	23.84558319922701
35	23.999393800352667	25.284553798544117	27.072089195077393	23.643963206025827
36	23.136898970632807	26.204235694441884	26.83773107175093	23.821134263174375
37	23.98633203999578	25.32976758439488	27.046384320528897	23.637516055080436
38	23.712704906365598	25.433759291851636	27.006026830195438	23.847508971587324
39	23.724929374391916	25.17026339542135	27.09369133720609	24.011115892980644
40	23.023445859840614	25.080338199118163	27.744769853457097	24.151446087584127
41	22.393634568786076	25.345090034044304	28.136790122629833	24.124485274539783
42	23.127102650365146	25.21112326115315	28.165090603403094	23.496683485078613
43	23.145690540103793	24.664287640058074	27.937765735653414	24.25225608418472
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	148.0
1	160.5
2	173.0
3	377.5
4	582.0
5	582.0
6	874.5
7	1167.0
8	1137.0
9	1107.0
10	1488.0
11	1869.0
12	1869.0
13	3457.5
14	5046.0
15	8302.0
16	11558.0
17	10818.0
18	10078.0
19	10078.0
20	11893.5
21	13709.0
22	13106.5
23	12504.0
24	15103.0
25	17702.0
26	17702.0
27	20533.0
28	23364.0
29	26644.5
30	29925.0
31	34097.0
32	38269.0
33	38269.0
34	42725.0
35	47181.0
36	52253.5
37	57326.0
38	59941.0
39	62556.0
40	62556.0
41	65540.0
42	68524.0
43	68786.0
44	69048.0
45	71764.0
46	74480.0
47	74480.0
48	77473.5
49	80467.0
50	79152.0
51	77837.0
52	79779.0
53	81721.0
54	81721.0
55	79348.5
56	76976.0
57	74986.0
58	72996.0
59	70566.0
60	68136.0
61	68136.0
62	62056.5
63	55977.0
64	50022.5
65	44068.0
66	38028.0
67	31988.0
68	31988.0
69	27727.0
70	23466.0
71	20030.0
72	16594.0
73	12182.5
74	7771.0
75	7771.0
76	5818.5
77	3866.0
78	3119.0
79	2372.0
80	1902.5
81	1433.0
82	1433.0
83	1091.0
84	749.0
85	662.0
86	575.0
87	554.0
88	533.0
89	533.0
90	452.5
91	372.0
92	225.0
93	78.0
94	54.0
95	30.0
96	30.0
97	16.5
98	3.0
99	2.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	1194326.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	61.790865349960924
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.92589706697463	53.09435535740031
2	8.892766942254564	10.98983529434871
3	2.1912241053435677	4.06192900934619
4	0.8568488819166908	2.1178173555111526
5	0.4521703495458776	1.396999859201704
6	0.29076807051261566	1.0780086415867787
7	0.1954833755611885	0.8455360856220069
8	0.14456952358973627	0.7146460772673116
9	0.11469812887034356	0.6378566973227877
>10	0.7662583291465628	9.154833155745063
>50	0.09055222844547806	3.887360890586669
>100	0.07344854365568824	8.660182843307497
>500	0.004360577791225219	1.71223162533948
>1k	8.176083358547286E-4	1.1575140473834193
>5k	1.362680559757881E-4	0.4908930600309402
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5830	0.4881414287221412	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	4555	0.3813866565745031	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3800	0.31817108561649	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1915	0.16034148130409956	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	1231	0.10307068589313136	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	1.674584661139421E-4	0.0
10	0.0	0.0	0.0	1.674584661139421E-4	0.0
11	0.0	0.0	0.0	1.674584661139421E-4	0.0
12	0.0	0.0	0.0	2.5118769917091316E-4	0.0
13	0.0	0.0	0.0	3.349169322278842E-4	0.0
14	0.0	0.0	0.0	3.349169322278842E-4	0.0
15	0.0	0.0	0.0	4.1864616528485525E-4	0.0
16	0.0	0.0	0.0	5.023753983418263E-4	0.0
17	0.0	0.0	0.0	7.535630975127394E-4	0.0
18	0.0	0.0	0.0	8.372923305697105E-4	0.0
19	0.0	0.0	0.0	0.0010884800297406236	0.0
20	0.0	0.0	0.0	0.0014233969619685078	0.0
21	0.0	0.0	0.0	0.0020095015933673053	0.0
22	0.0	0.0	0.0	0.0030979816231079286	0.0
23	0.0	0.0	0.0	0.005274941682589176	0.0
24	0.0	0.0	0.0	0.006949526343728597	0.0
25	0.0	0.0	0.0	0.008038006373469221	0.0
26	0.0	0.0	0.0	0.009963778733779554	0.0
27	0.0	0.0	0.0	0.016327200446109354	0.0
28	0.0	0.0	0.0	0.03608729944755452	0.0
29	0.0	0.0	0.0	0.06773694954308958	0.0
30	0.0	0.0	0.0	0.11429040312276548	0.0
31	0.0	0.0	0.0	0.26877083811287705	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TGTATCG	45	0.003825894	20.555555	5
TGTACGT	45	0.003825894	20.555555	12
AAGACGG	325	0.0	19.923077	5
GACGGAC	325	0.0	19.923077	7
CGGACCA	340	0.0	19.044117	9
GGTATCA	1795	0.0	18.860725	1
CGCAAGA	345	0.0	18.768116	2
CGTTAAC	60	9.2371047E-4	18.5	15
TTAGTCG	50	0.007035038	18.5	5
CGGTACT	60	9.2371047E-4	18.5	4
TATCGCG	50	0.007035038	18.5	7
GCGTACA	50	0.007035038	18.5	2
TACACGG	60	9.2371047E-4	18.5	5
AAGTCGC	60	9.2371047E-4	18.5	29
ACGGACC	370	0.0	18.0	8
AGACGGA	360	0.0	17.472221	6
CGTTATT	170	5.456968E-12	17.411764	2
CAAGACG	390	0.0	17.076923	4
GCGAAAG	370	0.0	17.0	18
GCGCAAG	385	0.0	16.818182	1
>>END_MODULE