FastQCFastQC Report
Fri 10 Feb 2017
ERR1630231.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630231.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences94329
Sequences flagged as poor quality0
Sequence length43
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT19852.104336948340383No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT17871.8944333131910653No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT11311.19899500683777No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7230.7664663041058424No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA2900.3074346171378897No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2790.29577330407403873No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT2450.25972924551304477No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA2360.2501881711880758No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA2290.2427673356019888No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA2000.21202387388819982No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA1980.2099036351493178No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA1960.20778339641043583No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1950.2067232770409948No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT1920.2035429189326718No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG1890.20036256082434883No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1780.18870124776049782No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA1700.18022029280496984No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC1680.17810005406608787No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA1630.17279945721888285No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC1570.16643874100223685No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA1540.16325838289391387No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA1440.15265718919950386No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC1330.14099587613565287No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA1320.1399357567662119No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG1260.1335750405495659No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA1250.1325149211801249No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC1220.12933456307180188No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1210.12827444370236088No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC1180.1250940855940379No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA1170.12403396622459688No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG1160.12297384685515589No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG1160.12297384685515589No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1160.12297384685515589No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA1130.11979348874683289No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA1110.11767325000795091No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC1090.11555301126906889No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA1050.1113125337913049No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA1030.1091922950524229No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1020.10813217568298189No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT1000.10601193694409991No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA990.1049518175746589No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA980.10389169820521792No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC980.10389169820521792No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC970.10283157883577691No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCT950.1007113400968949No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTTGG200.001833382337.01
TGCTGTG308.231287E-637.036
ATCCAGA200.001833382337.017
TATCACT250.005471111329.59999833
TCACTGT250.005471111329.59999835
GCTGTGA405.883378E-527.7537
CATGCTG451.310672E-424.66666634
AACTGTC400.001918142323.12536
TTTTGCG400.001918142323.1252
ATGCCCC400.001918142323.12537
GCACATG502.6767017E-422.19999931
ATGCTGT603.679815E-521.58333435
ACCATGC450.003800211220.5555558
GGCTCCT450.003800211220.55555524
ATATGCC801.5911857E-518.535
TATGCCC801.5911857E-518.536
ATACCAT701.2042068E-418.56
GGGCTCC500.00698826718.49999823
GAGAGGG500.00698826718.4999987
CGCCCTA1054.68297E-717.61904716
Produced by FastQC (version 0.11.5)