##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630231.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	94329
Sequences flagged as poor quality	0
Sequence length	43
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.695607925452407	31.0	31.0	34.0	30.0	34.0
2	31.840388427736965	33.0	31.0	34.0	30.0	34.0
3	31.839529731047715	33.0	31.0	34.0	30.0	34.0
4	35.585366112224236	37.0	35.0	37.0	33.0	37.0
5	35.565573683596774	37.0	35.0	37.0	33.0	37.0
6	35.66246859396368	37.0	35.0	37.0	33.0	37.0
7	35.64946092930064	37.0	35.0	37.0	33.0	37.0
8	35.71428722874196	37.0	35.0	37.0	33.0	37.0
9	37.35999533547477	39.0	37.0	39.0	34.0	39.0
10	37.27579005396007	39.0	37.0	39.0	34.0	39.0
11	37.37084035662416	39.0	37.0	39.0	34.0	39.0
12	37.30369239576376	39.0	37.0	39.0	34.0	39.0
13	37.33089505878362	39.0	37.0	39.0	34.0	39.0
14	38.32773590306269	40.0	38.0	41.0	34.0	41.0
15	38.37269556552068	40.0	38.0	41.0	34.0	41.0
16	38.211186379586344	40.0	37.0	41.0	33.0	41.0
17	38.32662277772477	40.0	38.0	41.0	34.0	41.0
18	38.357408644213336	40.0	38.0	41.0	34.0	41.0
19	38.4014884075947	40.0	38.0	41.0	34.0	41.0
20	38.47866509769	40.0	38.0	41.0	34.0	41.0
21	38.41212140487019	40.0	38.0	41.0	34.0	41.0
22	38.3565287451367	40.0	38.0	41.0	34.0	41.0
23	38.29432094053791	40.0	38.0	41.0	34.0	41.0
24	38.21091074855028	40.0	38.0	41.0	33.0	41.0
25	38.16235728142989	40.0	37.0	41.0	33.0	41.0
26	37.89131656224491	40.0	37.0	41.0	33.0	41.0
27	37.684550880429136	40.0	37.0	41.0	33.0	41.0
28	37.61410594832978	40.0	36.0	41.0	33.0	41.0
29	37.43426729849781	39.0	36.0	41.0	32.0	41.0
30	37.24932947449883	39.0	35.0	41.0	32.0	41.0
31	37.03011799128582	39.0	35.0	41.0	32.0	41.0
32	36.85156208589087	39.0	35.0	40.0	31.0	41.0
33	36.676207740991636	39.0	35.0	40.0	31.0	41.0
34	36.52825748179245	39.0	35.0	40.0	31.0	41.0
35	36.33024838596826	38.0	35.0	40.0	30.0	41.0
36	36.10320262061508	38.0	35.0	40.0	30.0	41.0
37	35.93371073582885	38.0	35.0	40.0	30.0	41.0
38	35.67708764006827	38.0	35.0	40.0	28.0	41.0
39	35.43500938205642	38.0	35.0	40.0	27.0	41.0
40	35.13867421471658	38.0	34.0	40.0	25.0	41.0
41	34.802701184153335	38.0	34.0	40.0	24.0	41.0
42	34.53379130490093	38.0	34.0	40.0	22.0	41.0
43	33.28053938873517	36.0	33.0	40.0	18.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
16	1.0
17	0.0
18	6.0
19	8.0
20	20.0
21	37.0
22	78.0
23	124.0
24	235.0
25	369.0
26	597.0
27	864.0
28	1177.0
29	1587.0
30	2030.0
31	2558.0
32	3134.0
33	3994.0
34	5086.0
35	6614.0
36	9477.0
37	14617.0
38	20119.0
39	21597.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.76323294002905	19.434108280592394	14.167435253209511	20.635223526169046
2	19.91752271305749	24.409248481379002	32.53294320940538	23.14028559615813
3	22.163915656902965	25.35381483955093	31.610639357991708	20.87163014555439
4	14.669931834324546	18.58177230756183	32.94638976348737	33.801906094626254
5	13.79639347390516	39.071759480117464	32.2361097859619	14.895737260015476
6	30.015159706983006	33.8315894369706	17.945700685897233	18.207550170149158
7	25.248863021976277	33.482810164424514	21.081533780703708	20.186793032895505
8	30.036362094371828	31.393314887256306	20.274782940559106	18.29554007781276
9	27.372282118966595	12.631322286889505	21.14832130097849	38.84807429316541
10	21.54904642262719	28.04333767982275	27.396664864463737	23.010951033086325
11	32.466155689130595	24.70290154671416	18.206490050779717	24.624452713375526
12	20.36489308696159	30.229303819610088	27.646853035651812	21.758950057776506
13	32.952750479704015	19.45001007113401	26.261277019792427	21.33596242936955
14	22.97278673578645	23.632180983578753	27.805870941067962	25.589161339566836
15	29.50948276775965	25.486329760731056	24.315957976868198	20.688229494641096
16	21.136659987914637	25.897656076074167	28.543714022198902	24.421969913812294
17	20.336269863986686	29.17024456953853	27.07014809867591	23.423337467798873
18	19.606907737811277	23.973539420538753	30.853714128210836	25.56583871343913
19	20.610840780671904	27.546141695554923	31.618060193577797	20.22495733019538
20	22.38760084385502	23.426517825907197	33.719216783809856	20.46666454642793
21	24.580987819228444	24.40818836200956	30.545219391703505	20.465604427058487
22	24.47285564354546	25.19691717287367	29.62397565965928	20.706251523921594
23	22.913420051097756	25.980345386890562	30.15509546374922	20.951139098262463
24	21.551166661366068	26.935512938756904	30.493273542600896	21.020046857276128
25	21.948711424906445	26.489202684222246	30.808128995324875	20.753956895546438
26	23.039574256061233	25.96656383508783	30.21234190969903	20.781519999151904
27	21.74198814786545	26.474361013050068	30.65653192549481	21.12711891358967
28	20.298105566686807	27.975490040178524	30.96714690074102	20.759257492393644
29	21.356104697388925	27.268390420761378	30.712718252075184	20.662786629774512
30	20.272662701820224	29.08543501998325	31.835384664313203	18.806517613883322
31	21.555407138843837	27.286412450041876	30.4805521101676	20.677628300946687
32	18.704746154416988	28.38469611678275	30.82403078586649	22.086526942933773
33	20.01929417252383	27.864177506387218	31.97001982423221	20.146508496856747
34	18.780014629647297	28.668808107792938	30.40528363493729	22.14589362762247
35	19.885719131974263	29.2031082699912	30.585503927742263	20.325668670292274
36	18.965535519299472	29.41089166640164	30.083007346627234	21.54056546767166
37	19.65249287069724	29.935650754274935	29.49994169343468	20.911914681593146
38	19.411845773834134	28.570217006434923	29.952612664185985	22.065324555544954
39	19.702318481060967	27.33517794103616	31.770717382777303	21.19178619512557
40	19.267669539590155	28.581878319498777	31.310625576439904	20.83982656447116
41	18.52028538413425	27.842975118998396	31.976380540448858	21.660358956418495
42	18.058073339057977	28.12920734874747	32.05800973189581	21.75470958029874
43	18.10683883005226	29.01228678349182	31.113443373723882	21.767431012732033
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	5.0
1	20.0
2	35.0
3	131.5
4	228.0
5	228.0
6	324.5
7	421.0
8	407.0
9	393.0
10	530.5
11	668.0
12	668.0
13	1300.5
14	1933.0
15	3071.5
16	4210.0
17	3334.5
18	2459.0
19	2459.0
20	2835.0
21	3211.0
22	2169.0
23	1127.0
24	1161.0
25	1195.0
26	1195.0
27	1261.0
28	1327.0
29	1318.5
30	1310.0
31	1428.0
32	1546.0
33	1546.0
34	1807.5
35	2069.0
36	2104.5
37	2140.0
38	2543.5
39	2947.0
40	2947.0
41	3648.5
42	4350.0
43	5183.0
44	6016.0
45	7148.0
46	8280.0
47	8280.0
48	9609.0
49	10938.0
50	9518.5
51	8099.0
52	7618.5
53	7138.0
54	7138.0
55	6762.0
56	6386.0
57	5825.5
58	5265.0
59	4478.0
60	3691.0
61	3691.0
62	3078.0
63	2465.0
64	1999.5
65	1534.0
66	1295.0
67	1056.0
68	1056.0
69	968.5
70	881.0
71	674.0
72	467.0
73	364.0
74	261.0
75	261.0
76	207.5
77	154.0
78	111.0
79	68.0
80	52.0
81	36.0
82	36.0
83	24.0
84	12.0
85	9.0
86	6.0
87	4.0
88	2.0
89	2.0
90	1.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	94329.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.98739518069735
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.05625533455759	30.76360398180835
2	11.194164963918993	9.176393261881287
3	4.065902801127693	4.999522946283752
4	2.2088301476864185	3.6213677660104526
5	1.3682331945270672	2.8040157321714427
6	1.0294079610997593	2.531565054225106
7	0.7061014406538552	2.025888115001749
8	0.5767788324754933	1.8912529550827424
9	0.462974937278535	1.7078523041694493
>10	3.0287354835372318	22.810588472261976
>50	0.20174326875824433	5.800973189581147
>100	0.09052582572485322	5.902744649047483
>500	0.0025864521635672348	0.7664663041058424
>1k	0.0077593564907017035	5.197765268369219
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	1985	2.104336948340383	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	1787	1.8944333131910653	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	1131	1.19899500683777	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	723	0.7664663041058424	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	290	0.3074346171378897	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	279	0.29577330407403873	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	245	0.25972924551304477	No Hit
GTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAA	236	0.2501881711880758	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	229	0.2427673356019888	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	200	0.21202387388819982	No Hit
GAATGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAAAAA	198	0.2099036351493178	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	196	0.20778339641043583	No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	195	0.2067232770409948	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	192	0.2035429189326718	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	189	0.20036256082434883	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	178	0.18870124776049782	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	170	0.18022029280496984	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	168	0.17810005406608787	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	163	0.17279945721888285	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	157	0.16643874100223685	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	154	0.16325838289391387	No Hit
GTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAA	144	0.15265718919950386	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	133	0.14099587613565287	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	132	0.1399357567662119	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	126	0.1335750405495659	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	125	0.1325149211801249	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	122	0.12933456307180188	No Hit
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	121	0.12827444370236088	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	118	0.1250940855940379	No Hit
ACGCAGAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAA	117	0.12403396622459688	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	116	0.12297384685515589	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	116	0.12297384685515589	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.12297384685515589	No Hit
GAGTACATGGGAATGGTATCAACGCAAAAAAAAAAAAAAAAAA	113	0.11979348874683289	No Hit
GTACATGGGAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAA	111	0.11767325000795091	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	109	0.11555301126906889	No Hit
CTCTATGGGCAGTCGGTGATTCCTCGCCTTATTGATATGCCCA	105	0.1113125337913049	No Hit
GAAGCAGAGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAA	103	0.1091922950524229	No Hit
CAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	102	0.10813217568298189	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	100	0.10601193694409991	No Hit
ACGCAGAGTACATGGGAAGCAGAGGTATCAACGCAAAAAAAAA	99	0.1049518175746589	No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	98	0.10389169820521792	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	98	0.10389169820521792	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	97	0.10283157883577691	No Hit
GTACTAGGCATCGTCATCCAATGCGACGAGTCCTACACTATCT	95	0.1007113400968949	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.001060119369440999	0.0
16	0.0	0.0	0.0	0.004240477477763996	0.0
17	0.0	0.0	0.0	0.006360716216645994	0.0
18	0.0	0.0	0.0	0.006360716216645994	0.0
19	0.0	0.0	0.0	0.008480954955527992	0.0
20	0.0	0.0	0.0	0.012721432433291989	0.0
21	0.0	0.0	0.0	0.019082148649937982	0.0
22	0.0	0.0	0.0	0.03286370045267097	0.0
23	0.0	0.0	0.0	0.048765490994285954	0.0
24	0.0	0.0	0.0	0.07632859459975193	0.0
25	0.0	0.0	0.0	0.08162919144695693	0.0
26	0.0	0.0	0.0	0.09965122072745392	0.0
27	0.0	0.0	0.0	0.1144928918996279	0.0
28	0.0	0.0	0.0	0.14205599550509387	0.0
29	0.0	0.0	0.0	0.19718220271602582	0.0
30	0.0	0.0	0.0	0.3392381982211197	0.0
31	0.0	0.0	0.0	0.6477329347284504	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTTGG	20	0.0018333823	37.0	1
TGCTGTG	30	8.231287E-6	37.0	36
ATCCAGA	20	0.0018333823	37.0	17
TATCACT	25	0.0054711113	29.599998	33
TCACTGT	25	0.0054711113	29.599998	35
GCTGTGA	40	5.883378E-5	27.75	37
CATGCTG	45	1.310672E-4	24.666666	34
AACTGTC	40	0.0019181423	23.125	36
TTTTGCG	40	0.0019181423	23.125	2
ATGCCCC	40	0.0019181423	23.125	37
GCACATG	50	2.6767017E-4	22.199999	31
ATGCTGT	60	3.679815E-5	21.583334	35
ACCATGC	45	0.0038002112	20.555555	8
GGCTCCT	45	0.0038002112	20.555555	24
ATATGCC	80	1.5911857E-5	18.5	35
TATGCCC	80	1.5911857E-5	18.5	36
ATACCAT	70	1.2042068E-4	18.5	6
GGGCTCC	50	0.006988267	18.499998	23
GAGAGGG	50	0.006988267	18.499998	7
CGCCCTA	105	4.68297E-7	17.619047	16
>>END_MODULE