FastQCFastQC Report
Fri 10 Feb 2017
ERR1630230.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630230.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences183343
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCTAGAACTGATCCAGCAATTACAACGGAGTCAAAAATTAAA1950.10635802839486644No Hit
GGTCTGAGCCATGCTGAGCTGCAATGAATCTCTTGCTTAAGAG1930.10526717682158578No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGCTA705.063426E-621.14285724
GGTATCA705.063426E-621.1428571
ACGCGCG806.894679E-720.812521
TCTTATA1202.3464963E-1020.04166837
AATGGAA656.861765E-519.92307734
GCGCGCT759.196292E-619.73333423
CGCTACA759.196292E-619.73333426
GCGCTAC759.196292E-619.73333425
CACGCGC851.2336659E-619.58823620
AGGAGTG609.196501E-418.55
GTATCAA801.6046246E-518.52
TACCCTA852.7029542E-517.4117645
AGGATTC752.0553479E-417.26666623
GAAATGG650.001573095117.07692332
GATTCCA650.001573095117.07692325
AAATGGA650.001573095117.07692333
CGCGCGC904.4132492E-516.44444522
CTACACT904.4132492E-516.44444528
TGCACGC904.4132492E-516.44444518
ATGGAAG803.363204E-416.187535
Produced by FastQC (version 0.11.5)