FastQCFastQC Report
Fri 10 Feb 2017
ERR1630229.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630229.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2966134
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT61830.20845315821874533No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT57560.19405731501004336No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG53370.1799311831495138No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT45580.15366804062122613No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43680.1472623961021316No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA38530.1298997280635332No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG37880.12770832335963245No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC35420.11941469940333106No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT29780.10040004935717671No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGCCAT9950.016.36180913
CGACGGT2300.016.0869567
CTTATAC9300.015.11827937
GACGGTA2600.014.9423078
GAGGTCG11250.014.89
CGAATTA2006.184564E-1114.79999915
AGGTCGC10900.014.76605510
GGTATCA26650.014.7166991
TACGACG2550.014.5098045
GTAAACG1803.3360266E-914.38888727
GTCGCCA11250.014.30666712
CGGCCTT11300.013.75221324
TCTAGCG2304.0017767E-1113.67391328
TTATACT4350.013.6091964
GGTCGCC11900.013.52520911
CTAGTAC1409.570191E-613.2142863
TTGGCCG11800.013.16949231
CTAGCGG2254.0745363E-1013.15555629
TCTTATA15900.013.14779937
TACGTTA1551.8901865E-613.12903219
Produced by FastQC (version 0.11.5)