##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630229.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2966134
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.413328258264798	31.0	31.0	34.0	30.0	34.0
2	31.6267764706517	31.0	31.0	34.0	30.0	34.0
3	31.759111692189226	31.0	31.0	34.0	30.0	34.0
4	35.50067158125695	37.0	35.0	37.0	33.0	37.0
5	35.32788943452993	37.0	35.0	37.0	33.0	37.0
6	35.39945936360259	37.0	35.0	37.0	33.0	37.0
7	35.38456219442547	37.0	35.0	37.0	33.0	37.0
8	35.35084355595533	37.0	35.0	37.0	33.0	37.0
9	37.00280196376833	39.0	37.0	39.0	33.0	39.0
10	36.85495631687577	39.0	37.0	39.0	33.0	39.0
11	36.961042892869976	39.0	37.0	39.0	33.0	39.0
12	36.82930305913354	39.0	37.0	39.0	33.0	39.0
13	36.87586096919424	39.0	37.0	39.0	33.0	39.0
14	37.99604839161009	40.0	37.0	41.0	33.0	41.0
15	37.97832431036494	40.0	37.0	41.0	33.0	41.0
16	37.941073464651296	40.0	37.0	41.0	33.0	41.0
17	37.89640589400209	40.0	37.0	41.0	33.0	41.0
18	37.85214120467922	39.0	37.0	41.0	33.0	41.0
19	37.87765185254611	40.0	37.0	41.0	33.0	41.0
20	37.81530301732828	39.0	37.0	41.0	33.0	41.0
21	37.772221686545514	39.0	37.0	41.0	33.0	41.0
22	37.74758625200345	39.0	37.0	41.0	33.0	41.0
23	37.696846804628514	39.0	37.0	40.0	32.0	41.0
24	37.683402031061306	39.0	37.0	41.0	32.0	41.0
25	37.646178830760846	39.0	37.0	41.0	32.0	41.0
26	37.46269049206813	39.0	37.0	40.0	32.0	41.0
27	37.35945510216329	39.0	36.0	40.0	32.0	41.0
28	37.28002005303874	39.0	36.0	40.0	32.0	41.0
29	37.20710628717381	39.0	36.0	40.0	31.0	41.0
30	37.150319911372854	39.0	36.0	40.0	31.0	41.0
31	37.027512243209515	39.0	36.0	40.0	31.0	41.0
32	36.92649758911769	39.0	36.0	40.0	31.0	41.0
33	36.872667923971065	39.0	36.0	40.0	30.0	41.0
34	36.764396686056664	39.0	36.0	40.0	30.0	41.0
35	36.689277692781246	39.0	35.0	40.0	30.0	41.0
36	36.58936784379937	39.0	35.0	40.0	30.0	41.0
37	36.52860086563857	39.0	35.0	40.0	30.0	41.0
38	36.411743029815916	38.0	35.0	40.0	30.0	41.0
39	36.314230914719296	38.0	35.0	40.0	30.0	41.0
40	36.22774021672655	38.0	35.0	40.0	30.0	41.0
41	36.09719217000985	38.0	35.0	40.0	29.0	41.0
42	36.054285814464215	38.0	35.0	40.0	29.0	41.0
43	34.83904773014301	38.0	33.0	40.0	25.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	3.0
10	9.0
11	5.0
12	1.0
13	8.0
14	6.0
15	20.0
16	44.0
17	87.0
18	177.0
19	379.0
20	710.0
21	1465.0
22	2554.0
23	4441.0
24	6954.0
25	10883.0
26	16277.0
27	23383.0
28	33115.0
29	44621.0
30	59994.0
31	77898.0
32	99572.0
33	128414.0
34	168218.0
35	225446.0
36	317446.0
37	483557.0
38	786090.0
39	474356.0
>>END_MODULE
>>Per base sequence content	warn
#Base	G	A	T	C
1	41.343445710814144	19.997039917953806	13.00804346668087	25.651470904551175
2	18.055859917319985	21.82578400031826	35.624587425922094	24.493768656439663
3	19.741387273804893	23.845517431107293	30.57825438769793	25.83484090738989
4	15.221092506272473	16.082011129638783	34.72941546133789	33.96748090275085
5	14.180512411104825	37.302832576006345	34.49213690278322	14.024518110105614
6	34.081467661272214	35.55233849853041	15.971126051621404	14.395067788575972
7	28.443185641646668	31.224516491837523	21.70380704310729	18.628490823408516
8	26.01301896677628	35.36118058051322	20.27457289522321	18.351227557487288
9	26.277504657577843	14.93738988191363	19.777596022297036	39.00750943821149
10	16.361533228100956	27.759568515785194	33.7028603562752	22.176037899838647
11	34.98034815689379	22.34491091771309	21.961145383182284	20.71359554221084
12	20.617038879565115	26.125758310312346	29.848786332647144	23.408416477475395
13	29.404908881392412	20.313579898952643	25.737137971514436	24.54437324814051
14	22.266593484987528	21.372972360655318	25.309915196009353	31.0505189583478
15	24.665709640899568	28.559094093523758	22.853451664692155	23.921744600884516
16	24.480519086460692	27.43004867615556	24.057409409015236	24.03202282836851
17	23.2720099631372	27.292226177239463	25.68673566332472	23.749028196298617
18	22.617319379367217	26.19520898246674	27.15625794384205	24.03121369432399
19	23.9727200456891	26.310443155973402	27.13643415975138	22.58040263858612
20	24.173756141833106	25.597292637487044	27.163540150242703	23.065411070437143
21	23.658742322497904	26.63362477892098	26.02862851105176	23.679004387529357
22	23.76848112728555	26.009445291412998	26.309431738417754	23.9126418428837
23	23.200098174930737	26.49671255580496	26.406932390782075	23.896256878482227
24	24.38672696513374	26.406763821189465	26.16584415943447	23.040665054242325
25	24.24145369022438	25.7572651808718	26.471056263810066	23.530224865093754
26	24.04078844718411	26.268604183088158	26.134018220350125	23.556589149377608
27	24.054948292963164	25.84060598745707	26.01187269354655	24.092573026033214
28	23.302116492376946	26.20805398542345	26.701558324741903	23.7882711974577
29	23.37443284760567	26.69117443783727	26.399886181811073	23.53450653274599
30	23.14865073526685	27.369431050653816	26.252252932605202	23.229665281474134
31	23.922284023580865	26.323018447581937	26.467212877098607	23.287484651738595
32	23.066085348807572	26.488958354545005	26.494790862449236	23.950165434198183
33	23.26004152206205	26.126702300030953	26.685072218584864	23.928183959322137
34	23.54333957939864	26.07626627792271	26.620914631638353	23.759479511040297
35	23.958020777213708	26.088268432916383	26.527425935578098	23.426284854291815
36	23.445602929604664	26.49492571812332	26.495937135678965	23.563534216593045
37	24.344921706167018	25.306240378890504	26.40271815096688	23.9461197639756
38	23.59080877667698	26.013389819880018	27.098506001414634	23.297295402028364
39	23.527392895937947	25.677464335731294	27.181947949755475	23.613194818575288
40	23.299048525791484	25.115689311406697	27.895435607427043	23.689826555374776
41	22.676183881105842	25.869667385222648	27.88538885970762	23.568759873963888
42	23.461583326983877	25.096674661360545	27.512816346125966	23.928925665529608
43	22.761244097535716	25.2693236381094	27.682565925881974	24.286866338472908
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	299.0
1	303.5
2	308.0
3	688.5
4	1069.0
5	1069.0
6	1436.0
7	1803.0
8	1782.0
9	1761.0
10	2737.5
11	3714.0
12	3714.0
13	6056.5
14	8399.0
15	13377.5
16	18356.0
17	18748.0
18	19140.0
19	19140.0
20	23253.0
21	27366.0
22	31019.5
23	34673.0
24	40035.0
25	45397.0
26	45397.0
27	50841.5
28	56286.0
29	72416.5
30	88547.0
31	93876.0
32	99205.0
33	99205.0
34	108811.0
35	118417.0
36	126456.5
37	134496.0
38	153158.5
39	171821.0
40	171821.0
41	183779.0
42	195737.0
43	186952.0
44	178167.0
45	189056.5
46	199946.0
47	199946.0
48	207942.5
49	215939.0
50	222120.5
51	228302.0
52	235654.5
53	243007.0
54	243007.0
55	215315.0
56	187623.0
57	176037.5
58	164452.0
59	149141.0
60	133830.0
61	133830.0
62	122839.5
63	111849.0
64	98683.5
65	85518.0
66	74333.0
67	63148.0
68	63148.0
69	54450.5
70	45753.0
71	39363.0
72	32973.0
73	26984.5
74	20996.0
75	20996.0
76	16529.0
77	12062.0
78	9852.5
79	7643.0
80	5716.5
81	3790.0
82	3790.0
83	2771.5
84	1753.0
85	1431.5
86	1110.0
87	933.5
88	757.0
89	757.0
90	654.5
91	552.0
92	338.0
93	124.0
94	80.0
95	36.0
96	36.0
97	21.0
98	6.0
99	5.0
100	4.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2966134.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	50.98710289913123
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.13325534615156	41.8773674177485
2	10.417418695363514	10.623079979276659
3	3.062670089390527	4.684700249815387
4	1.3195186512377108	2.691137329919201
5	0.7024038518956217	1.79067687366741
6	0.46519579930797833	1.4231391652535699
7	0.2902373586063487	1.0358853447903766
8	0.22134230660852783	0.9028482370384051
9	0.17296693937414287	0.7937174822415495
>10	1.0009118512506416	9.446799062169292
>50	0.09917092262421734	3.5248999639422345
>100	0.0932258425196615	10.050240806769231
>500	0.015582113860404097	5.35531463168688
>1k	0.0059013112066866125	5.216127298407582
>5k	1.9892060247258246E-4	0.5840661572737131
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	6183	0.20845315821874533	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5756	0.19405731501004336	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	5337	0.1799311831495138	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	4558	0.15366804062122613	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4368	0.1472623961021316	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	3853	0.1298997280635332	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	3788	0.12770832335963245	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	3542	0.11941469940333106	No Hit
CTACCAGAAGACAGCAGAAATGAAAAGCATTTACTTTGTGGCT	2978	0.10040004935717671	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	3.3713918521550276E-5	0.0
5	0.0	0.0	0.0	3.3713918521550276E-5	0.0
6	0.0	0.0	0.0	3.3713918521550276E-5	0.0
7	0.0	0.0	0.0	3.3713918521550276E-5	0.0
8	0.0	0.0	0.0	3.3713918521550276E-5	0.0
9	0.0	0.0	0.0	6.742783704310055E-5	0.0
10	0.0	0.0	0.0	1.348556740862011E-4	0.0
11	0.0	0.0	0.0	1.6856959260775137E-4	0.0
12	0.0	0.0	0.0	1.6856959260775137E-4	0.0
13	0.0	0.0	0.0	1.6856959260775137E-4	0.0
14	0.0	0.0	0.0	2.0228351112930163E-4	0.0
15	0.0	0.0	0.0	2.3599742965085192E-4	0.0
16	0.0	0.0	0.0	3.034252666939525E-4	0.0
17	0.0	0.0	0.0	5.394226963448044E-4	0.0
18	0.0	3.3713918521550276E-5	0.0	6.06850533387905E-4	0.0
19	0.0	1.0114175556465082E-4	0.0	6.405644519094552E-4	0.0
20	0.0	1.0114175556465082E-4	0.0	7.754201259956563E-4	0.0
21	0.0	1.0114175556465082E-4	0.0	0.0010788453926896088	0.0
22	0.0	1.0114175556465082E-4	0.0	0.0014496984964266618	0.0
23	0.0	1.0114175556465082E-4	0.0	0.002359974296508519	0.0
24	0.0	1.0114175556465082E-4	0.0	0.003236536178068826	0.0
25	0.0	1.0114175556465082E-4	0.0	0.00367481711884898	0.0
26	0.0	1.0114175556465082E-4	0.0	0.005293085207883393	0.0
27	0.0	1.0114175556465082E-4	0.0	0.010114175556465082	0.0
28	0.0	1.0114175556465082E-4	0.0	0.025588864157856658	0.0
29	0.0	1.348556740862011E-4	0.0	0.04925603495998495	0.0
30	0.0	1.348556740862011E-4	0.0	0.08172253849623787	0.0
31	0.0	1.348556740862011E-4	0.0	0.20295778949973264	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGCCAT	995	0.0	16.361809	13
CGACGGT	230	0.0	16.086956	7
CTTATAC	930	0.0	15.118279	37
GACGGTA	260	0.0	14.942307	8
GAGGTCG	1125	0.0	14.8	9
CGAATTA	200	6.184564E-11	14.799999	15
AGGTCGC	1090	0.0	14.766055	10
GGTATCA	2665	0.0	14.716699	1
TACGACG	255	0.0	14.509804	5
GTAAACG	180	3.3360266E-9	14.388887	27
GTCGCCA	1125	0.0	14.306667	12
CGGCCTT	1130	0.0	13.752213	24
TCTAGCG	230	4.0017767E-11	13.673913	28
TTATACT	435	0.0	13.609196	4
GGTCGCC	1190	0.0	13.525209	11
CTAGTAC	140	9.570191E-6	13.214286	3
TTGGCCG	1180	0.0	13.169492	31
CTAGCGG	225	4.0745363E-10	13.155556	29
TCTTATA	1590	0.0	13.147799	37
TACGTTA	155	1.8901865E-6	13.129032	19
>>END_MODULE