FastQCFastQC Report
Fri 10 Feb 2017
ERR1630228.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630228.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences85520
Sequences flagged as poor quality0
Sequence length43
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG1240.1449953227315248No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT1170.13681010289990644No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA1060.12394761459307764No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG1000.11693171188026193No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC920.10757717492984097No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG900.10523854069223575No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA890.10406922357343311No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC870.10173058933582786No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTTATC250.005468286629.62
GTTATCT250.005468286629.63
CGCGCAC601.3133831E-624.66666821
GCGCACT601.3133831E-624.66666822
CTACCCT400.001916683423.1254
AGGCGGC652.6349535E-622.7692331
TCTTATA502.6738647E-422.237
AACTGGC502.6738647E-422.27
TCCGCGC603.6746333E-521.5833348
ATCCGCG603.6746333E-521.5833347
CACTGGA806.80644E-720.812525
CAAGTAC555.089785E-420.18181814
GATCCGC656.807133E-519.9230776
CGCCTAG656.807133E-519.92307712
TGCTGAT656.807133E-519.9230772
GCCTAGA656.807133E-519.92307713
TAGAGTT701.2025354E-418.516
TATCAAC500.00698305318.51
TGTCACC609.141852E-418.512
GGCCGTT500.00698305318.513