##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630228.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	85520
Sequences flagged as poor quality	0
Sequence length	43
%GC	53
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.568685687558467	31.0	30.0	33.0	27.0	34.0
2	30.77134003741815	31.0	31.0	33.0	27.0	34.0
3	30.925327408793265	31.0	31.0	34.0	27.0	34.0
4	34.77135173058934	35.0	35.0	37.0	32.0	37.0
5	34.16717726847521	35.0	35.0	37.0	30.0	37.0
6	34.29643358278765	35.0	35.0	37.0	30.0	37.0
7	34.28774555659495	35.0	35.0	37.0	30.0	37.0
8	34.240844246959774	35.0	35.0	37.0	30.0	37.0
9	35.80401075771749	37.0	35.0	39.0	30.0	39.0
10	35.443568755846584	37.0	35.0	39.0	30.0	39.0
11	35.65798643592142	37.0	35.0	39.0	30.0	39.0
12	35.42957202993452	37.0	35.0	39.0	30.0	39.0
13	35.57749064546305	37.0	35.0	39.0	30.0	39.0
14	36.443159494855	38.0	35.0	40.0	31.0	41.0
15	36.36089803554724	38.0	35.0	40.0	30.0	41.0
16	36.32934985968195	38.0	35.0	40.0	30.0	41.0
17	36.165376520112254	38.0	35.0	40.0	30.0	41.0
18	36.12554957904584	38.0	34.0	40.0	30.0	41.0
19	36.115189429373245	38.0	34.0	40.0	30.0	41.0
20	35.997789990645465	38.0	34.0	40.0	29.0	41.0
21	35.93512628624883	38.0	34.0	40.0	29.0	41.0
22	35.877093077642655	38.0	34.0	40.0	29.0	41.0
23	35.81033676333021	38.0	34.0	40.0	29.0	41.0
24	35.73587464920487	38.0	34.0	40.0	29.0	41.0
25	35.673959307764264	38.0	34.0	40.0	28.0	41.0
26	35.430051449953226	38.0	34.0	40.0	27.0	41.0
27	35.25844246959775	38.0	34.0	40.0	27.0	40.0
28	35.14727549111319	38.0	33.0	40.0	27.0	40.0
29	34.96775023386343	38.0	33.0	40.0	26.0	40.0
30	34.87654349859682	37.0	33.0	39.0	26.0	40.0
31	34.70999766136576	37.0	33.0	39.0	25.0	40.0
32	34.57930308699719	37.0	33.0	39.0	25.0	40.0
33	34.5231407857811	37.0	33.0	39.0	25.0	40.0
34	34.34135874649205	37.0	32.0	39.0	25.0	40.0
35	34.254992984097285	37.0	32.0	39.0	25.0	40.0
36	34.127747895229184	37.0	32.0	39.0	24.0	40.0
37	34.007553788587465	37.0	32.0	39.0	24.0	40.0
38	33.86715388213283	37.0	32.0	39.0	24.0	40.0
39	33.695813844714685	36.0	31.0	39.0	23.0	40.0
40	33.57697614593078	36.0	31.0	39.0	23.0	40.0
41	33.401976145930774	36.0	31.0	39.0	23.0	40.0
42	33.28717259120673	36.0	31.0	39.0	23.0	40.0
43	31.89721702525725	35.0	29.0	38.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	1.0
15	3.0
16	2.0
17	6.0
18	24.0
19	53.0
20	76.0
21	155.0
22	251.0
23	389.0
24	557.0
25	852.0
26	1128.0
27	1517.0
28	2045.0
29	2540.0
30	3397.0
31	4078.0
32	5105.0
33	6627.0
34	8213.0
35	10424.0
36	12421.0
37	13251.0
38	9744.0
39	2660.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.28718428437792	19.135874649204865	12.226379794200188	26.35056127221703
2	20.981057062675397	20.85360149672591	31.417212347988777	26.74812909260992
3	21.797240411599624	21.266370439663238	28.20860617399439	28.727782974742748
4	16.227782974742748	15.570626753975677	32.65434985968195	35.547240411599624
5	16.16580916744621	34.96608980355472	33.552385406922355	15.315715622076706
6	38.603835360149674	33.56057062675398	14.131197380729654	13.704396632366697
7	32.72801683816651	28.272918615528532	19.333489242282507	19.66557530402245
8	29.355706267539755	32.428671655753035	18.676333021515433	19.53928905519177
9	28.15130963517306	12.69644527595884	17.340972871842844	41.811272217025255
10	17.725678203928904	25.287652011225443	32.00420954162769	24.98246024321796
11	40.21281571562207	19.721702525724975	19.19200187090739	20.873479887745557
12	23.458840037418145	23.421421889616465	27.500000000000004	25.619738072965387
13	33.631898971000936	16.97380729653882	22.69995322731525	26.694340505144996
14	24.717025257249766	18.457670720299348	22.51286248830683	34.312441534144064
15	26.805425631431245	26.11085126286249	20.28531337698784	26.798409728718433
16	28.797942001870908	23.49158091674462	21.45112254443405	26.25935453695042
17	26.592609915809167	24.108980355472404	22.546772684752106	26.751637043966326
18	26.47100093545369	22.643826005612723	24.2855472404116	26.599625818521982
19	28.478718428437794	23.412067352666043	22.79583723105706	25.313376987839103
20	28.298643592142188	22.685921421889617	23.046071094480823	25.969363891487372
21	28.082319925163706	23.123246024321798	22.024087932647333	26.770346117867167
22	27.835594013096355	22.646164639850326	22.730355472404113	26.787885874649202
23	26.297942001870904	22.800514499532273	23.367633302151543	27.533910196445277
24	27.12698783910196	23.279934518241348	22.681244153414408	26.91183348924228
25	27.411131898971004	23.08933582787652	22.8122076707203	26.687324602432177
26	27.8589803554724	23.09401309635173	22.00537885874649	27.04162768942937
27	27.77829747427502	22.953695042095415	22.048643592142188	27.21936389148737
28	27.459073900841908	23.001637043966326	22.711646398503273	26.827642656688493
29	27.04162768942937	23.51262862488307	23.7511693171188	25.694574368568755
30	27.21819457436857	23.072965388213284	22.74438727782975	26.964452759588397
31	27.15154349859682	22.961880261927035	22.54911131898971	27.337464920486436
32	26.17867165575304	23.443638914873713	22.960710944808234	27.416978484565014
33	26.658091674462113	22.58068288119738	23.285781103835358	27.475444340505145
34	27.773620205799816	22.457904583723106	23.467025257249766	26.301449953227312
35	28.08465855940131	22.85196445275959	22.662535079513564	26.40084190832554
36	26.135406922357348	23.499766136576238	22.836763330215152	27.528063610851262
37	26.354069223573433	22.5456033676333	24.46913002806361	26.631197380729652
38	26.418381665107578	22.459073900841908	23.753507951356408	27.36903648269411
39	26.134237605238543	22.698783910196447	25.011693171188025	26.155285313376986
40	26.986669784845652	21.949251637043965	24.828110383536014	26.235968194574365
41	25.194106641721238	22.433348924228248	25.50397567820393	26.868568755846585
42	25.542563143124415	22.780636108512628	25.261927034611787	26.41487371375117
43	25.091206735266603	21.145930776426567	25.730823199251635	28.032039289055195
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	2.0
5	2.0
6	3.5
7	5.0
8	3.5
9	2.0
10	6.0
11	10.0
12	10.0
13	18.0
14	26.0
15	62.5
16	99.0
17	90.5
18	82.0
19	82.0
20	99.0
21	116.0
22	141.5
23	167.0
24	238.0
25	309.0
26	309.0
27	404.5
28	500.0
29	623.5
30	747.0
31	953.0
32	1159.0
33	1159.0
34	1508.5
35	1858.0
36	2228.0
37	2598.0
38	2934.0
39	3270.0
40	3270.0
41	3563.0
42	3856.0
43	4207.5
44	4559.0
45	4876.5
46	5194.0
47	5194.0
48	5504.0
49	5814.0
50	6054.0
51	6294.0
52	6853.0
53	7412.0
54	7412.0
55	7473.5
56	7535.0
57	7165.5
58	6796.0
59	6816.5
60	6837.0
61	6837.0
62	6404.5
63	5972.0
64	5130.0
65	4288.0
66	3690.5
67	3093.0
68	3093.0
69	2737.0
70	2381.0
71	2267.5
72	2154.0
73	1801.0
74	1448.0
75	1448.0
76	928.0
77	408.0
78	321.0
79	234.0
80	191.5
81	149.0
82	149.0
83	116.5
84	84.0
85	58.0
86	32.0
87	23.0
88	14.0
89	14.0
90	13.0
91	12.0
92	8.0
93	4.0
94	2.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	85520.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	75.63026192703461
#Duplication Level	Percentage of deduplicated	Percentage of total
1	92.54317475533017	69.99064546304959
2	3.8884336492524625	5.881665107577175
3	1.0405231991836608	2.3608512628624885
4	0.5751480387761098	1.7399438727782977
5	0.3896164133644614	1.4733395696913003
6	0.30767327880765005	1.3961646398503276
7	0.14842530032931864	0.78578110383536
8	0.15615578472147065	0.9448082319925164
9	0.10822678149012817	0.7366697848456502
>10	0.7854172142426445	11.582086061739943
>50	0.05256729386663368	2.7022918615528533
>100	0.0046382906352912074	0.4057530402245089
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG	124	0.1449953227315248	No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT	117	0.13681010289990644	No Hit
AGCAGGAACTGGCCAAGTACTTCTTGGCAGAGCTGCTGTCTGA	106	0.12394761459307764	No Hit
GGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGG	100	0.11693171188026193	No Hit
CTTTAGGAGCGAGGTTCGGAGCCATCGCTGCTGCCTGCTGATC	92	0.10757717492984097	No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG	90	0.10523854069223575	No Hit
ACGATGGACAGCGCAGCCAGCGCGCACTGGAGGCGGCAGGACA	89	0.10406922357343311	No Hit
CTGCTGATCCGCGCCTAGAGTTTGACCAGCCACTCTCCAGCTC	87	0.10173058933582786	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	0.0	0.0
14	0.0	0.0	0.0	0.0	0.0
15	0.0	0.0	0.0	0.0	0.0
16	0.0	0.0	0.0	0.0	0.0
17	0.0	0.0	0.0	0.0	0.0
18	0.0	0.0	0.0	0.0	0.0
19	0.0	0.0	0.0	0.0	0.0
20	0.0	0.0	0.0	0.0	0.0
21	0.0	0.0	0.0	0.0011693171188026192	0.0
22	0.0	0.0	0.0	0.0011693171188026192	0.0
23	0.0	0.0	0.0	0.004677268475210477	0.0
24	0.0	0.0	0.0	0.004677268475210477	0.0
25	0.0	0.0	0.0	0.004677268475210477	0.0
26	0.0	0.0	0.0	0.005846585594013096	0.0
27	0.0	0.0	0.0	0.009354536950420954	0.0
28	0.0	0.0	0.0	0.025724976613657625	0.0
29	0.0	0.0	0.0	0.05963517305893358	0.0
30	0.0	0.0	0.0	0.10406922357343311	0.0
31	0.0	0.0	0.0	0.29466791393826003	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGTTATC	25	0.0054682866	29.6	2
GTTATCT	25	0.0054682866	29.6	3
CGCGCAC	60	1.3133831E-6	24.666668	21
GCGCACT	60	1.3133831E-6	24.666668	22
CTACCCT	40	0.0019166834	23.125	4
AGGCGGC	65	2.6349535E-6	22.76923	31
TCTTATA	50	2.6738647E-4	22.2	37
AACTGGC	50	2.6738647E-4	22.2	7
TCCGCGC	60	3.6746333E-5	21.583334	8
ATCCGCG	60	3.6746333E-5	21.583334	7
CACTGGA	80	6.80644E-7	20.8125	25
CAAGTAC	55	5.089785E-4	20.181818	14
GATCCGC	65	6.807133E-5	19.923077	6
CGCCTAG	65	6.807133E-5	19.923077	12
TGCTGAT	65	6.807133E-5	19.923077	2
GCCTAGA	65	6.807133E-5	19.923077	13
TAGAGTT	70	1.2025354E-4	18.5	16
TATCAAC	50	0.006983053	18.5	1
TGTCACC	60	9.141852E-4	18.5	12
GGCCGTT	50	0.006983053	18.5	13
>>END_MODULE