FastQCFastQC Report
Fri 10 Feb 2017
ERR1630227.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630227.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491312
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT97560.39160089141785537No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT76610.3075086540746402No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT66330.26624525551195516No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG44000.17661376816713442No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT32920.13213921018322877No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT28780.11552146017841203No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC28080.11271169568484397No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG27300.10958081524915386No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA26320.10564714495815859No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT25050.10054942937697084No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAAGACG5550.021.6666664
AAGACGG5800.020.732765
CGGACCA5450.020.3669729
GACGGAC5550.020.3333347
ACGGACC5650.019.9734528
AGACGGA6150.018.6504066
TAGGTCG500.00703713218.521
GACGCGT500.00703713218.525
GCGCAAG6600.017.9393941
CGCAAGA6600.017.3787882
GCTTAGG11300.017.0265481
CGGCCTT7850.016.96815324
TTAGGAC13450.016.7806683
CGAACGA3100.016.70967716
GGTATCA36900.016.5447161
ATCTCGC8200.016.24390411
CTTAGGA12800.016.18752
CGAGCCG5300.016.05660415
ACGAACG3250.015.93846115
TCTCGCC8600.015.91860412
Produced by FastQC (version 0.11.5)