##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630227.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2491312
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.862981433076225	31.0	31.0	34.0	30.0	34.0
2	32.05057776785886	33.0	31.0	34.0	30.0	34.0
3	32.19496554425941	34.0	31.0	34.0	30.0	34.0
4	35.8464114490678	37.0	35.0	37.0	35.0	37.0
5	35.76813381864656	37.0	35.0	37.0	35.0	37.0
6	35.83325693449877	37.0	35.0	37.0	35.0	37.0
7	35.80483937780575	37.0	35.0	37.0	35.0	37.0
8	35.78638966135113	37.0	35.0	37.0	35.0	37.0
9	37.49335370278793	39.0	37.0	39.0	35.0	39.0
10	37.41247824439492	39.0	37.0	39.0	34.0	39.0
11	37.49387672037866	39.0	37.0	39.0	35.0	39.0
12	37.381548758244655	39.0	37.0	39.0	34.0	39.0
13	37.413034979159576	39.0	37.0	39.0	34.0	39.0
14	38.62736220914923	40.0	38.0	41.0	34.0	41.0
15	38.62540380329722	40.0	38.0	41.0	34.0	41.0
16	38.60709015972307	40.0	38.0	41.0	34.0	41.0
17	38.590482444591444	40.0	38.0	41.0	34.0	41.0
18	38.56278980713777	40.0	38.0	41.0	34.0	41.0
19	38.58882307796053	40.0	38.0	41.0	34.0	41.0
20	38.54485307340068	40.0	38.0	41.0	34.0	41.0
21	38.49416572472657	40.0	38.0	41.0	34.0	41.0
22	38.47437253944909	40.0	38.0	41.0	34.0	41.0
23	38.418161595175555	40.0	38.0	41.0	34.0	41.0
24	38.40377078422935	40.0	38.0	41.0	34.0	41.0
25	38.37213363882163	40.0	38.0	41.0	34.0	41.0
26	38.21839536758142	40.0	38.0	41.0	34.0	41.0
27	38.12322944697412	40.0	38.0	41.0	33.0	41.0
28	38.031033848831456	40.0	38.0	41.0	33.0	41.0
29	37.96036546205373	40.0	37.0	41.0	33.0	41.0
30	37.892870102179096	40.0	37.0	41.0	33.0	41.0
31	37.783868901205466	40.0	37.0	41.0	33.0	41.0
32	37.683891058205475	40.0	37.0	41.0	33.0	41.0
33	37.60837582767634	40.0	37.0	41.0	32.0	41.0
34	37.52670480453673	40.0	37.0	41.0	32.0	41.0
35	37.4327302240747	40.0	37.0	41.0	32.0	41.0
36	37.32036252384286	40.0	36.0	41.0	31.0	41.0
37	37.26145380426057	39.0	36.0	41.0	31.0	41.0
38	37.140060739080454	39.0	36.0	41.0	31.0	41.0
39	37.04338637633504	39.0	36.0	41.0	31.0	41.0
40	36.94052972891392	39.0	36.0	41.0	31.0	41.0
41	36.802821966899366	39.0	35.0	41.0	30.0	41.0
42	36.73545746177115	39.0	35.0	41.0	30.0	41.0
43	35.55874454905688	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	7.0
11	7.0
12	6.0
13	4.0
14	6.0
15	10.0
16	22.0
17	72.0
18	113.0
19	196.0
20	416.0
21	839.0
22	1373.0
23	2424.0
24	4059.0
25	6050.0
26	9397.0
27	13603.0
28	19503.0
29	27476.0
30	36986.0
31	49492.0
32	64710.0
33	84528.0
34	113046.0
35	152259.0
36	215479.0
37	330337.0
38	591533.0
39	767358.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.61919020981716	19.326282697630806	12.544635115954966	25.50989197659707
2	17.63179401054545	21.71911827984612	35.24881668775328	25.400271021855154
3	19.110613203003076	23.48674112274978	31.24068763767846	26.161958036568684
4	14.010368833771123	15.784855529937639	35.62107034365828	34.58370529263296
5	13.657141297436853	37.6537743967837	34.6715706422961	14.017513663483339
6	33.50098261478289	36.24692531485418	16.17665711881932	14.075434951543606
7	28.34976108973906	31.886532076271457	21.384395049676634	18.379311784312844
8	27.041815717983138	34.45337235962417	20.20822763266905	18.296584289723647
9	26.305175746755122	14.506332406378647	19.832241003936883	39.35625084292935
10	16.511862022901987	27.570733814150937	33.04287058385301	22.874533579094066
11	35.304690861682516	22.317277001033993	21.770777807035007	20.607254330248477
12	20.962809957163135	26.406808942436754	29.442237664331085	23.188143436069026
13	29.38319247047339	19.650449241203027	26.089586531112925	24.87677175721066
14	22.306318919509078	21.24900454057942	25.410145337075406	31.034531202836096
15	24.702526219116674	28.381029754603198	22.798790356246027	24.1176536700341
16	24.517723994425427	27.337563500677554	24.064950516033317	24.079761988863698
17	23.195609381723365	27.36357389198944	25.617425677715193	23.823391048572
18	22.694788930491246	25.725601610717565	27.460791743466896	24.118817715324294
19	23.769162593846136	26.178656065559032	27.279080259718576	22.773101080876263
20	24.16008111388698	25.16284592214865	27.289837643779663	23.387235320184708
21	24.332118979878874	26.180301784762406	26.035879889793005	23.45169934556571
22	24.168711104831512	25.65419345308817	26.38685961453242	23.790235827547896
23	23.081011129878554	26.01023075391601	26.776292973340954	24.132465142864483
24	23.943889805853303	26.23248312535724	26.588359868214017	23.23526720057544
25	23.979493535936086	25.60919708169832	26.60004046060871	23.81126892175689
26	23.691211698896	26.510970926162603	26.438920536648965	23.358896838292434
27	24.151732099391808	25.563719036395284	26.230636708690042	24.053912155522873
28	23.013657061018453	26.114553295612914	27.234726120212965	23.63706352315567
29	22.93791383817041	26.553318091029947	26.978034063979138	23.530734006820502
30	22.71554104825088	27.36622309851195	26.82506245705074	23.09317339618643
31	23.76113469529308	26.539991779431883	26.426958967804914	23.27191455747012
32	22.842381845389095	26.376945159819403	26.444901321070986	24.33577167372051
33	22.777195309138317	26.021550090875813	27.06208616182959	24.13916843815628
34	23.503559570218425	25.95804941332117	26.903575304899586	23.634815711560815
35	23.91940471526649	25.95680508904545	27.090625341185685	23.033164854502367
36	22.831223066400355	26.775329625514587	26.80310615450815	23.59034115357691
37	24.172965891064628	25.428689782732956	26.624084016775097	23.774260309427323
38	23.23671222231499	25.822859601687785	27.543318540592267	23.39710963540496
39	23.505646823842216	25.59105403096842	27.195830951723433	23.70746819346593
40	23.077117599080324	25.293459831606803	28.074123192920037	23.555299376392842
41	22.193286107882113	25.863079373438573	28.44685852273822	23.496775995941093
42	23.36543957561317	25.10978151271298	28.109205109596868	23.415573802076977
43	22.45519629817542	24.824389719151995	28.290073663997124	24.430340318675462
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	247.0
1	256.5
2	266.0
3	736.0
4	1206.0
5	1206.0
6	1720.0
7	2234.0
8	2113.5
9	1993.0
10	2880.0
11	3767.0
12	3767.0
13	6697.5
14	9628.0
15	16018.5
16	22409.0
17	21128.0
18	19847.0
19	19847.0
20	22966.5
21	26086.0
22	26910.0
23	27734.0
24	31867.5
25	36001.0
26	36001.0
27	40804.5
28	45608.0
29	57008.0
30	68408.0
31	73739.5
32	79071.0
33	79071.0
34	87655.0
35	96239.0
36	104892.0
37	113545.0
38	127423.5
39	141302.0
40	141302.0
41	151472.0
42	161642.0
43	154795.5
44	147949.0
45	157858.5
46	167768.0
47	167768.0
48	174348.0
49	180928.0
50	183168.5
51	185409.0
52	193696.0
53	201983.0
54	201983.0
55	182733.5
56	163484.0
57	153806.0
58	144128.0
59	131053.5
60	117979.0
61	117979.0
62	107830.0
63	97681.0
64	85220.5
65	72760.0
66	62699.0
67	52638.0
68	52638.0
69	44541.0
70	36444.0
71	31675.5
72	26907.0
73	21439.0
74	15971.0
75	15971.0
76	12121.0
77	8271.0
78	6876.0
79	5481.0
80	4308.5
81	3136.0
82	3136.0
83	2331.0
84	1526.0
85	1424.5
86	1323.0
87	1237.0
88	1151.0
89	1151.0
90	1060.0
91	969.0
92	554.0
93	139.0
94	92.5
95	46.0
96	46.0
97	24.0
98	2.0
99	4.0
100	6.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2491312.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	47.19777886668537
#Duplication Level	Percentage of deduplicated	Percentage of total
1	81.12299562052947	38.28825208300835
2	11.395049755957587	10.756420771131266
3	3.133796648765551	4.437247237247884
4	1.3417087131256815	2.5330268458244367
5	0.659516271382927	1.556385156785612
6	0.41483808560918	1.1747661738036703
7	0.2873929946860556	0.9495017707718856
8	0.20562157307444195	0.7763905228949999
9	0.15914107480614997	0.6759994731576571
>10	0.9952557568807382	9.046708345877429
>50	0.12232728704734584	4.074621866601592
>100	0.137560876283768	14.201200232965395
>500	0.017723130945192705	5.664219914208463
>1k	0.006816588825074106	4.8980652506734685
>5k	2.55622080940279E-4	0.9671943550478423
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	9756	0.39160089141785537	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	7661	0.3075086540746402	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	6633	0.26624525551195516	No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG	4400	0.17661376816713442	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3292	0.13213921018322877	No Hit
GTTCTACAGCACACTACCAGAAGACAGCAGAAATGAAAAGCAT	2878	0.11552146017841203	No Hit
CTTCTGGGAAATCTCGCCTTCCTCGGCCTTTCACCAGCCAAGC	2808	0.11271169568484397	No Hit
CTGTAGAACAGAGCAGGTGAAGAGAGAGCAAGCCCTCTTTGGG	2730	0.10958081524915386	No Hit
TCCCAGAAGAGGTCGCCATTGTTGAAGAACTTGGCCGCAGACA	2632	0.10564714495815859	No Hit
GCGCAAGACGGACCAGAGCGAAAGCATTTGCCAAGAATGTTTT	2505	0.10054942937697084	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	4.013949276525782E-5	0.0	0.0	0.0	0.0
6	4.013949276525782E-5	0.0	0.0	0.0	0.0
7	4.013949276525782E-5	0.0	0.0	0.0	0.0
8	4.013949276525782E-5	0.0	0.0	0.0	0.0
9	4.013949276525782E-5	0.0	0.0	0.0	0.0
10	4.013949276525782E-5	0.0	0.0	4.013949276525782E-5	0.0
11	4.013949276525782E-5	4.013949276525782E-5	0.0	8.027898553051564E-5	0.0
12	4.013949276525782E-5	4.013949276525782E-5	0.0	1.6055797106103128E-4	0.0
13	4.013949276525782E-5	4.013949276525782E-5	0.0	2.0069746382628912E-4	0.0
14	8.027898553051564E-5	4.013949276525782E-5	0.0	2.0069746382628912E-4	0.0
15	8.027898553051564E-5	4.013949276525782E-5	0.0	2.8097644935680475E-4	0.0
16	8.027898553051564E-5	4.013949276525782E-5	0.0	6.422318842441251E-4	0.0
17	1.2041847829577347E-4	4.013949276525782E-5	0.0	8.830688408356721E-4	0.0
18	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.001123905797427219	0.0
19	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.0013246032612535081	0.0
20	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.001605579710610313	0.0
21	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.0021675326093239227	0.0
22	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.0030506014501595945	0.0
23	1.2041847829577347E-4	4.013949276525782E-5	0.0	0.004575902175239392	0.0
24	1.6055797106103128E-4	4.013949276525782E-5	0.0	0.0062617608713802205	0.0
25	1.6055797106103128E-4	4.013949276525782E-5	0.0	0.007104690219450635	0.0
26	1.6055797106103128E-4	4.013949276525782E-5	0.0	0.009352501814305072	0.0
27	2.0069746382628912E-4	4.013949276525782E-5	0.0	0.01802263225160076	0.0
28	2.0069746382628912E-4	4.013949276525782E-5	0.0	0.04379218660689629	0.0
29	2.0069746382628912E-4	4.013949276525782E-5	0.0	0.07819173190672224	0.0
30	2.0069746382628912E-4	4.013949276525782E-5	0.0	0.13005195655943536	0.0
31	2.4083695659154694E-4	4.013949276525782E-5	0.0	0.2696571123970021	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAAGACG	555	0.0	21.666666	4
AAGACGG	580	0.0	20.73276	5
CGGACCA	545	0.0	20.366972	9
GACGGAC	555	0.0	20.333334	7
ACGGACC	565	0.0	19.973452	8
AGACGGA	615	0.0	18.650406	6
TAGGTCG	50	0.007037132	18.5	21
GACGCGT	50	0.007037132	18.5	25
GCGCAAG	660	0.0	17.939394	1
CGCAAGA	660	0.0	17.378788	2
GCTTAGG	1130	0.0	17.026548	1
CGGCCTT	785	0.0	16.968153	24
TTAGGAC	1345	0.0	16.780668	3
CGAACGA	310	0.0	16.709677	16
GGTATCA	3690	0.0	16.544716	1
ATCTCGC	820	0.0	16.243904	11
CTTAGGA	1280	0.0	16.1875	2
CGAGCCG	530	0.0	16.056604	15
ACGAACG	325	0.0	15.938461	15
TCTCGCC	860	0.0	15.918604	12
>>END_MODULE