FastQCFastQC Report
Fri 10 Feb 2017
ERR1630226.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630226.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences496239
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT84381.700390336108206No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT63691.2834541420565493No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT43050.8675255270142007No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT18720.3772375810849208No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA13010.26217205822194545No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA12170.2452447308655708No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC12070.2432295728469548No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA11730.2363780355836603No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA11190.2254961822831337No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA10460.21078552874723672No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG9770.1968809384187861No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC9610.19365668558900045No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA9310.18761121153315238No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG9090.18317786389219712No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC8950.18035664266613466No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA8820.17773693724193382No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA8770.1767293582326258No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC8280.16685508394140727No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT8230.16584750493209927No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8180.16483992592279123No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA8080.16282476790417522No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC7950.16020506247997437No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG7550.15214443040551026No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC7460.1503307881887558No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT7450.15012927238689422No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA7340.1479125985664166No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA6740.13582165045472042No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG6690.1348140714454124No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC6550.13199285021934995No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC6420.1293731447951491No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA6200.12493979715419384No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC5870.11828977569276093No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT5700.1148640070611137No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA5600.11284884904249766No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT5560.11204278583505127No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT5160.10398215376058713No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA5110.10297457475127911No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG5100.10277305894941752No Hit
CACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCG5090.10257154314755593No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG5060.1019669957419711No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG5030.10136244833638629No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTGC250.005493371329.59999816
ACGTTTA454.002224E-628.77777726
TCACGTT454.002224E-628.77777724
TAAGGGT405.9334307E-527.7500024
TCGTGGT706.5738277E-926.4285735
GTTATGG358.8622636E-426.428571
AATCGGC358.8622636E-426.4285725
ATCACGT509.077499E-625.89999823
AGTCCTA659.3694325E-825.61538529
CGTGGTT809.749783E-1025.43750236
TCTTATC451.3217324E-424.6666662
TAGCACA601.334849E-624.6666664
TTAGGTC701.9183426E-723.78571320
ATTAGGT701.9183426E-723.78571319
GCACACA551.8998348E-523.5454546
TATACTG400.001929648823.1250025
TACACTA652.6777452E-622.7692334
GAGTCCT753.7319114E-722.19999928
TTAGAGC502.6991047E-422.1999993
CAATGCG753.7319114E-722.19999919
Produced by FastQC (version 0.11.5)