##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630226.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	496239
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.59668425899617	31.0	31.0	34.0	30.0	34.0
2	31.75181515358527	31.0	31.0	34.0	30.0	34.0
3	31.826444515646696	33.0	31.0	34.0	30.0	34.0
4	35.52718548925014	37.0	35.0	37.0	33.0	37.0
5	35.49037258256606	37.0	35.0	37.0	33.0	37.0
6	35.58341242828556	37.0	35.0	37.0	33.0	37.0
7	35.579817386380356	37.0	35.0	37.0	33.0	37.0
8	35.59558196755999	37.0	35.0	37.0	33.0	37.0
9	37.19317304766453	39.0	37.0	39.0	34.0	39.0
10	37.119186521011045	39.0	37.0	39.0	33.0	39.0
11	37.21290547498282	39.0	37.0	39.0	34.0	39.0
12	37.082998313312736	39.0	37.0	39.0	33.0	39.0
13	37.14991163532088	39.0	37.0	39.0	33.0	39.0
14	38.17557467268796	40.0	38.0	41.0	33.0	41.0
15	38.207202577790135	40.0	38.0	41.0	33.0	41.0
16	38.08121086815023	40.0	37.0	41.0	33.0	41.0
17	38.15146330699522	40.0	37.0	41.0	33.0	41.0
18	38.17928659375825	40.0	38.0	41.0	33.0	41.0
19	38.169654138429266	40.0	38.0	41.0	33.0	41.0
20	38.15849620848019	40.0	38.0	41.0	33.0	41.0
21	38.109767672432035	40.0	37.0	41.0	33.0	41.0
22	38.07762187171907	40.0	37.0	41.0	33.0	41.0
23	38.02407912316444	40.0	37.0	41.0	33.0	41.0
24	37.96438409717898	40.0	37.0	41.0	33.0	41.0
25	37.90945693506556	40.0	37.0	41.0	33.0	41.0
26	37.63374301495852	39.0	37.0	41.0	33.0	41.0
27	37.47011822932095	39.0	36.0	41.0	32.0	41.0
28	37.386785399777125	39.0	36.0	41.0	32.0	41.0
29	37.28190448554023	39.0	36.0	41.0	32.0	41.0
30	37.109812005908445	39.0	36.0	40.0	31.0	41.0
31	36.90467496508739	39.0	35.0	40.0	31.0	41.0
32	36.73876095994068	39.0	35.0	40.0	31.0	41.0
33	36.62121880787282	38.0	35.0	40.0	31.0	41.0
34	36.43358744475948	38.0	35.0	40.0	30.0	41.0
35	36.291389834333856	38.0	35.0	40.0	30.0	41.0
36	36.0566682586415	38.0	35.0	40.0	30.0	41.0
37	35.903635143549785	38.0	35.0	40.0	29.0	41.0
38	35.68666509484341	38.0	35.0	40.0	28.0	41.0
39	35.473809998811056	38.0	35.0	40.0	27.0	41.0
40	35.22443016369129	38.0	34.0	40.0	26.0	41.0
41	34.95788924288498	38.0	34.0	40.0	25.0	41.0
42	34.76212470200851	38.0	34.0	40.0	24.0	41.0
43	33.54944492472377	36.0	32.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	1.0
10	1.0
11	5.0
12	5.0
13	2.0
14	1.0
15	3.0
16	5.0
17	7.0
18	19.0
19	48.0
20	110.0
21	242.0
22	438.0
23	801.0
24	1346.0
25	2151.0
26	3118.0
27	4579.0
28	6068.0
29	8278.0
30	10719.0
31	13633.0
32	17470.0
33	22207.0
34	28457.0
35	37439.0
36	51450.0
37	78155.0
38	113998.0
39	95482.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.13969679932452	20.983235900443134	13.670428966687423	22.206638333544927
2	19.71167119069642	22.80090843323479	33.06592186426299	24.421498511805805
3	22.71546573324547	23.440116556739795	30.284600766969145	23.55981694304559
4	15.101795707310389	18.602125185646432	33.33837122837988	32.95770787866331
5	14.182480619217754	38.24749767753038	32.28585419525672	15.28416750799514
6	30.459314967183154	34.62686326548297	17.3962546273066	17.517567140027285
7	26.483005164850006	32.82007258599183	20.054449569663007	20.642472679495164
8	28.76557465253638	32.23487069738574	19.922658235245517	19.07689641483237
9	26.66396635492172	13.791136932002523	21.134574267641195	38.41032244543456
10	22.08915462105961	26.670616376383155	28.749654904189313	22.490574098367926
11	34.83563363621158	23.17270508766945	19.31226687140672	22.679394404712244
12	20.424432581880907	27.459147709067604	28.421385662956762	23.695034046094722
13	33.21181930481079	19.22642113981368	25.802284786161504	21.759474769214027
14	22.242911177880014	23.312154022557678	26.927549023756697	27.517385775805607
15	29.54906809017429	26.17730569342595	22.916981535107077	21.356644681292682
16	22.5631197870381	26.215996727383377	26.93641571903861	24.284467766539912
17	21.333873395682325	27.900064283540793	26.651472375206303	24.114589945570582
18	21.743756536668823	24.262301028335138	29.298785464262178	24.695156970733862
19	21.463448056279333	27.474059878405367	29.511989182631755	21.550502882683546
20	23.30852673812417	23.833475401973644	31.385078560935355	21.47291929896683
21	23.66077635977825	25.09355371101425	29.118025789992323	22.127644139215178
22	23.694832530292864	25.67976317862965	28.162840889168322	22.46256340190916
23	23.553569953187882	25.68177833664827	28.681341047358227	22.083310662805626
24	22.7037778167375	25.666463135706785	28.953387379871394	22.67637166768432
25	23.109630641686767	26.45479295258938	29.31893704444834	21.116639361275514
26	23.65271572770379	26.36330477854421	28.62491662283698	21.35906287091502
27	22.82206759243026	26.13478585923315	29.74010507033909	21.303041477997496
28	21.44954346595088	27.973013003814696	28.54431030209234	22.033133228142084
29	21.647633499180838	27.735022841816143	28.033266228571314	22.584077430431705
30	21.151098563393848	28.85968253200575	30.060515195299036	19.928703709301367
31	22.628612422643123	26.38829273797505	28.33836115258978	22.644733686792048
32	20.273094214682843	27.50992969113673	28.638821213165432	23.578154881014996
33	21.958370865651432	26.342548650952462	29.917035944373577	21.78204453902253
34	20.05747230669093	27.118384488119634	29.17283808809868	23.651305117090757
35	21.985575498902747	27.716080356441154	28.965478327983092	21.332865816673014
36	21.150695531790127	27.667716563994365	29.074498376790213	22.107089527425295
37	21.86789027061557	27.896840030711008	28.324255046459466	21.911014652213954
38	21.36269015534853	26.794347078726176	28.68396075278243	23.15900201314286
39	21.717962514030535	25.54232940176004	30.19150046650908	22.548207617700342
40	21.099309002315415	26.772583372125126	29.66977605548939	22.458331570070065
41	20.48609641725056	25.280761890943676	30.33316607521779	23.899975616587973
42	19.995606955519417	25.93226247836224	30.39019504714462	23.68193551897372
43	20.513704082105598	26.480385459425797	29.348156835718274	23.657753622750327
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	137.0
1	122.5
2	108.0
3	241.5
4	375.0
5	375.0
6	592.5
7	810.0
8	856.5
9	903.0
10	1422.5
11	1942.0
12	1942.0
13	3867.0
14	5792.0
15	9390.0
16	12988.0
17	11612.5
18	10237.0
19	10237.0
20	13153.0
21	16069.0
22	11747.0
23	7425.0
24	7066.5
25	6708.0
26	6708.0
27	7271.0
28	7834.0
29	7778.5
30	7723.0
31	8052.0
32	8381.0
33	8381.0
34	10205.5
35	12030.0
36	12119.5
37	12209.0
38	13799.0
39	15389.0
40	15389.0
41	17967.5
42	20546.0
43	25378.5
44	30211.0
45	35852.5
46	41494.0
47	41494.0
48	48948.5
49	56403.0
50	51629.0
51	46855.0
52	43705.5
53	40556.0
54	40556.0
55	39465.5
56	38375.0
57	34659.0
58	30943.0
59	26239.0
60	21535.0
61	21535.0
62	17832.5
63	14130.0
64	11568.5
65	9007.0
66	7812.0
67	6617.0
68	6617.0
69	6078.5
70	5540.0
71	4365.5
72	3191.0
73	2562.0
74	1933.0
75	1933.0
76	1501.0
77	1069.0
78	771.5
79	474.0
80	339.5
81	205.0
82	205.0
83	135.5
84	66.0
85	43.0
86	20.0
87	13.0
88	6.0
89	6.0
90	3.5
91	1.0
92	1.0
93	1.0
94	0.5
95	0.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	496239.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.24655298351104
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.97844090130192	20.04896291605632
2	12.168709982432173	6.874482225195003
3	4.977545545806829	4.21795511962416
4	2.6743485838120176	3.021645158760955
5	1.6287741034227914	2.300362700525128
6	1.123390049205315	1.9039137927616208
7	0.808204042420726	1.598028481400739
8	0.6382886423993759	1.4423563165045843
9	0.5116526757615673	1.3007181973548876
>10	3.661114221640185	20.7484827221561
>50	0.49157407513902324	9.70838924458985
>100	0.30866279136636343	16.71964490642777
>500	0.022149413269345525	4.454589377319706
>1k	0.005715977617895619	2.672107799733278
>5k	0.0014289944044739047	2.9883610415899278
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	8438	1.700390336108206	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	6369	1.2834541420565493	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	4305	0.8675255270142007	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1872	0.3772375810849208	No Hit
AGCGTACACGGTGGATGCCCTGGCAGTCAGAGGCGATGAAGGA	1301	0.26217205822194545	No Hit
ACCCTGTATCGCGCGCCTTTCCAGACGCTTCCACTAACACACA	1217	0.2452447308655708	No Hit
GGTTAATGAGGCGAACCGGGGGAACTGAAACATCTAAGTACCC	1207	0.2432295728469548	No Hit
GTACTGGTTCACTATCGGTCAGTCAGGAGTATTTAGCCTTGGA	1173	0.2363780355836603	No Hit
ATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGGGGAA	1119	0.2254961822831337	No Hit
ACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAAAAAAA	1046	0.21078552874723672	No Hit
ACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTATG	977	0.1968809384187861	No Hit
GATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAACCC	961	0.19365668558900045	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATATTGTGA	931	0.18761121153315238	No Hit
GTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCGCGCGATACAG	909	0.18317786389219712	No Hit
CATCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCC	895	0.18035664266613466	No Hit
GTGGTATCCTGTCTGAATATGGGGGGACCATCCTCCAAGGCTA	882	0.17773693724193382	No Hit
ATACAGGGTGACAGCCCCGTACACAAAAATGCACATGCTGTGA	877	0.1767293582326258	No Hit
GTGGATGCCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATC	828	0.16685508394140727	No Hit
GGATACCACGTGTCCCGCCCTACTCATCGAGCTCACAATATGT	823	0.16584750493209927	No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	818	0.16483992592279123	No Hit
GGTGATATGAACCGTTATAACCGGCGATTTCCGAATGGGGAAA	808	0.16282476790417522	No Hit
GGGTTTCCCCATTCGGAAATCGCCGGTTATAACGGTTCATATC	795	0.16020506247997437	No Hit
GTTAATGATAGTGTGTCGAAACACACTGGGTTTCCCCATTCGG	755	0.15214443040551026	No Hit
GTTATAACCGGCGATTTCCGAATGGGGAAACCCAGTGTGTTTC	746	0.1503307881887558	No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTT	745	0.15012927238689422	No Hit
GGTATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAA	734	0.1479125985664166	No Hit
TATCAACGCAGAGTACATGGGAAAAAAAAAAAAAAAAAAAAAA	674	0.13582165045472042	No Hit
TCTAAGTACCCCGAGGAAAAGAAATCAACCGAGATTCCCCCAG	669	0.1348140714454124	No Hit
GGGTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACC	655	0.13199285021934995	No Hit
GATTAGCACGTCCTTCATCGCCTCTGACTGCCAGGGCATCCAC	642	0.1293731447951491	No Hit
ATCCATAGGTTAATGAGGCGAACCGGGGGAACTGAAACATCTA	620	0.12493979715419384	No Hit
TCATTAACCTATGGATTCAGTTAATGATAGTGTGTCGAAACAC	587	0.11828977569276093	No Hit
GTGCTAATCTGCGATAAGCGTCGGTAAGGTGATATGAACCGTT	570	0.1148640070611137	No Hit
CCCTGGCAGTCAGAGGCGATGAAGGACGTGCTAATCTGCGATA	560	0.11284884904249766	No Hit
GTATTTAGCCTTGGAGGATGGTCCCCCCATATTCAGACAGGAT	556	0.11204278583505127	No Hit
CTACTGGGGGAATCTCGGTTGATTTCTTTTCCTCGGGGTACTT	516	0.10398215376058713	No Hit
GTACTTAGATGTTTCAGTTCCCCCGGTTCGCCTCATTAACCTA	511	0.10297457475127911	No Hit
CACTATCATTAACTGAATCCATAGGTTAATGAGGCGAACCGGG	510	0.10277305894941752	No Hit
CACACACACTGATTCAGGCTCTGGGCTCCTCCCCGTTCGCTCG	509	0.10257154314755593	No Hit
GCCCAGAGCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGG	506	0.1019669957419711	No Hit
GCCTGAATCAGTGTGTGTGTTAGTGGAAGCGTCTGGAAAGGCG	503	0.10136244833638629	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	2.0151580186160298E-4	0.0	0.0	0.0	0.0
11	2.0151580186160298E-4	0.0	0.0	0.0	0.0
12	2.0151580186160298E-4	0.0	0.0	0.0	0.0
13	2.0151580186160298E-4	0.0	0.0	0.0	0.0
14	2.0151580186160298E-4	0.0	0.0	4.0303160372320596E-4	0.0
15	2.0151580186160298E-4	0.0	0.0	4.0303160372320596E-4	0.0
16	2.0151580186160298E-4	0.0	0.0	6.045474055848089E-4	0.0
17	2.0151580186160298E-4	0.0	0.0	0.0010075790093080148	0.0
18	2.0151580186160298E-4	0.0	0.0	0.0012090948111696178	0.0
19	2.0151580186160298E-4	0.0	0.0	0.0018136422167544268	0.0
20	2.0151580186160298E-4	0.0	0.0	0.002619705424200839	0.0
21	2.0151580186160298E-4	0.0	0.0	0.0034257686316472506	0.0
22	2.0151580186160298E-4	0.0	0.0	0.006650021461432898	0.0
23	2.0151580186160298E-4	0.0	0.0	0.010075790093080149	0.0
24	4.0303160372320596E-4	0.0	0.0	0.015113685139620223	0.0
25	4.0303160372320596E-4	0.0	0.0	0.01692732735637465	0.0
26	4.0303160372320596E-4	0.0	0.0	0.02176370660105312	0.0
27	4.0303160372320596E-4	0.0	0.0	0.03748193914625816	0.0
28	4.0303160372320596E-4	0.0	0.0	0.07556842569810111	0.0
29	4.0303160372320596E-4	0.0	0.0	0.13380649243610437	0.0
30	4.0303160372320596E-4	0.0	0.0	0.21118856035095993	0.0
31	4.0303160372320596E-4	0.0	0.0	0.4280195631540447	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTGC	25	0.0054933713	29.599998	16
ACGTTTA	45	4.002224E-6	28.777777	26
TCACGTT	45	4.002224E-6	28.777777	24
TAAGGGT	40	5.9334307E-5	27.750002	4
TCGTGGT	70	6.5738277E-9	26.42857	35
GTTATGG	35	8.8622636E-4	26.42857	1
AATCGGC	35	8.8622636E-4	26.42857	25
ATCACGT	50	9.077499E-6	25.899998	23
AGTCCTA	65	9.3694325E-8	25.615385	29
CGTGGTT	80	9.749783E-10	25.437502	36
TCTTATC	45	1.3217324E-4	24.666666	2
TAGCACA	60	1.334849E-6	24.666666	4
TTAGGTC	70	1.9183426E-7	23.785713	20
ATTAGGT	70	1.9183426E-7	23.785713	19
GCACACA	55	1.8998348E-5	23.545454	6
TATACTG	40	0.0019296488	23.125002	5
TACACTA	65	2.6777452E-6	22.76923	34
GAGTCCT	75	3.7319114E-7	22.199999	28
TTAGAGC	50	2.6991047E-4	22.199999	3
CAATGCG	75	3.7319114E-7	22.199999	19
>>END_MODULE