FastQCFastQC Report
Fri 10 Feb 2017
ERR1630225.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630225.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1312110
Sequences flagged as poor quality0
Sequence length43
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATATGGACAATACTAGTTAAGAAAGCTAACAGGATGTGAAAG23750.1810061656416002No Hit
GTCCATATCAGACCTCTGATCCCTCGCCCCCACACCCCATCTC23260.17727172264520505No Hit
GTCCCAGGCTGCTGAGCAGGATGAAATGAGGCTTGAGCTGCAG22890.17445183711731488No Hit
GCCTGGGACAGATCTTCAGGTTCCAGGGCATCATTCTCCGTCT22240.16949798416291317No Hit
GCCCAGGGCCAGGACGATGGACAGCGCAGCCAGCGCGCACTGG20200.15395050719832942No Hit
GCCCTGGGCTGTGTCACCGGCGCTCCCTCGGACCCCAGACTCC16480.12559922567467666No Hit
AGCTAACAGGATGTGAAAGTCTTCCAGAAGAAATTCTTGCAGC16080.12255070077966024No Hit
GTCTGAACCCAACCAGACGGAGAATGATGCCCTGGAACCTGAA16010.12201720892303237No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT15980.12178856955590614No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT15190.1157677328882487No Hit
GGATGAAATGAGGCTTGAGCTGCAGAGATCTGCTAACTCAAAC14650.11165222427997654No Hit
GAGATGGGGTGTGGGGGCGAGGGATCAGAGGTCTGATATGGAC13990.10662215820319942No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAC556.2628897E-726.9090928
GCGAACT809.786163E-1025.43750229
GGTATCA9850.024.2284281
TTCCGCG753.7416066E-722.19999925
CTAGCGG1101.7553248E-920.18181829
CTTATAC5450.018.66972537
TCTAGCG1002.875695E-718.528
CGCAAGA1700.018.52
ACTATAC1054.7985486E-717.6190473
CGCGTAA953.6071724E-617.52631610
AAGACGG1800.017.4722215
GTATCAA14050.016.722422
TCTTATA9700.016.2113437
TCCGCGA1059.345036E-615.85714226
GTATAGA1302.589568E-715.6538461
ACGGACC2055.456968E-1215.3414638
TATTAGA1701.4861143E-915.2352942
AAGGCGT1101.4521011E-515.1363648
TCTTCCG1353.9730912E-715.07407523
TTAGAGT2109.094947E-1214.976194
Produced by FastQC (version 0.11.5)