FastQCFastQC Report
Fri 10 Feb 2017
ERR1630224.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630224.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences678911
Sequences flagged as poor quality0
Sequence length43
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT51330.7560637550430027No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT39060.5753331438141376No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT31420.46279998409217116No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13670.2013518708637804No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC8840.13020852512332248No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT7150.10531571884974614No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA6930.10207523519283088No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTATA200.001841404937.02
CTTAAGG250.00549477929.59999819
AACCGCG250.00549477929.59999835
TCACATT250.00549477929.59999826
ATAAGGT502.7180067E-729.5999983
GCGGATC454.00508E-628.77777733
TTGTGCA405.9366008E-527.75000219
ACTGTGC405.9366008E-527.7500028
TGCGGAT509.083933E-625.89999832
CGCCGAA809.767973E-1025.43750233
TTAGAGT601.3360604E-624.6666684
GAGGCAC1103.6379788E-1223.5454547
CGTCTAA903.8216967E-922.611111
GCCGAAT903.8216967E-922.6111134
TTAGGTT1155.456968E-1222.521744
GTGATCG502.700524E-422.1999998
GTTGTGC502.700524E-422.19999918
CTCGCTA502.700524E-422.19999913
GAGCATG502.700524E-422.1999997
GCCGTTT502.700524E-422.19999912