##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630224.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	678911
Sequences flagged as poor quality	0
Sequence length	43
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.752586126900287	31.0	31.0	34.0	30.0	34.0
2	31.944120805230728	33.0	31.0	34.0	30.0	34.0
3	32.06643433380811	33.0	31.0	34.0	30.0	34.0
4	35.74068176830247	37.0	35.0	37.0	33.0	37.0
5	35.686618717328194	37.0	35.0	37.0	33.0	37.0
6	35.77094052092248	37.0	35.0	37.0	35.0	37.0
7	35.762563870669354	37.0	35.0	37.0	35.0	37.0
8	35.75335795118948	37.0	35.0	37.0	33.0	37.0
9	37.43742257821717	39.0	37.0	39.0	34.0	39.0
10	37.33504686181252	39.0	37.0	39.0	34.0	39.0
11	37.419131520921006	39.0	37.0	39.0	34.0	39.0
12	37.29423444310079	39.0	37.0	39.0	34.0	39.0
13	37.36523638591804	39.0	37.0	39.0	34.0	39.0
14	38.51444298295358	40.0	38.0	41.0	34.0	41.0
15	38.50959256809803	40.0	38.0	41.0	34.0	41.0
16	38.47050791635428	40.0	38.0	41.0	34.0	41.0
17	38.4785413699292	40.0	38.0	41.0	34.0	41.0
18	38.47554244959943	40.0	38.0	41.0	34.0	41.0
19	38.51527077923321	40.0	38.0	41.0	34.0	41.0
20	38.46596976628748	40.0	38.0	41.0	34.0	41.0
21	38.4412610784035	40.0	38.0	41.0	34.0	41.0
22	38.428307981458545	40.0	38.0	41.0	34.0	41.0
23	38.38298097983388	40.0	38.0	41.0	34.0	41.0
24	38.37590936072622	40.0	38.0	41.0	34.0	41.0
25	38.33797508068068	40.0	38.0	41.0	34.0	41.0
26	38.15256786235604	40.0	38.0	41.0	34.0	41.0
27	38.06082535118742	40.0	37.0	41.0	33.0	41.0
28	37.99955958881208	40.0	37.0	41.0	33.0	41.0
29	37.94009376781345	40.0	37.0	41.0	33.0	41.0
30	37.82933403642009	40.0	37.0	41.0	33.0	41.0
31	37.682478262982926	40.0	37.0	41.0	33.0	41.0
32	37.583762820163464	40.0	37.0	41.0	33.0	41.0
33	37.53556651755532	40.0	36.0	41.0	32.0	41.0
34	37.46297084595772	39.0	36.0	41.0	32.0	41.0
35	37.353857869440915	39.0	36.0	41.0	32.0	41.0
36	37.234639002755884	39.0	36.0	41.0	31.0	41.0
37	37.14892231824201	39.0	36.0	41.0	31.0	41.0
38	37.043426899844015	39.0	35.0	41.0	31.0	41.0
39	36.94606067658353	39.0	35.0	41.0	31.0	41.0
40	36.82087637407554	39.0	35.0	41.0	31.0	41.0
41	36.66619041376558	39.0	35.0	41.0	30.0	41.0
42	36.57413416486108	39.0	35.0	40.0	30.0	41.0
43	35.443949206891624	38.0	34.0	40.0	27.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	0.0
9	1.0
10	2.0
11	2.0
12	2.0
13	3.0
14	0.0
15	1.0
16	1.0
17	6.0
18	11.0
19	20.0
20	71.0
21	155.0
22	281.0
23	535.0
24	930.0
25	1528.0
26	2434.0
27	3779.0
28	5516.0
29	7603.0
30	10396.0
31	14163.0
32	18602.0
33	24330.0
34	33009.0
35	43725.0
36	61307.0
37	96158.0
38	164180.0
39	190158.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.10478251199347	20.571474022368175	12.886225145858587	24.437518319779766
2	19.010297373293405	21.720372773456315	34.451496587918	24.817833265332276
3	18.680504513846437	25.375049159610025	30.391465155226534	25.552981171317008
4	13.265361733717674	17.46046241701784	38.30870320262892	30.96547264663557
5	12.423425161766417	38.909223742139986	36.1321292481636	12.535221847929995
6	29.97815619425816	38.84662348967685	16.860383761641806	14.314836554423186
7	28.136825003571897	31.012901543795873	22.869124229832778	17.981149222799452
8	27.519954751064574	33.284627882005154	19.9248502381019	19.27056712882837
9	26.5965642035554	13.938351271374305	20.714055303272445	38.75102922179785
10	18.591538507992947	26.769193605641977	32.73654425985144	21.902723626513637
11	34.10034599527773	24.063389752117732	21.53036259539174	20.305901657212804
12	20.699325832104652	28.153469305991507	28.14536809684922	23.001836765054623
13	30.337113406617362	20.173336416702632	25.318782579749037	24.17076759693097
14	22.50088745063786	21.700782576803146	26.36560609564435	29.432723876914647
15	25.94021896831838	28.1889673315059	22.479529717444553	23.391283982731167
16	23.382299005318814	26.194007756539516	25.832693828793463	24.590999409348203
17	21.941904019819976	27.835901907613813	26.33128642782338	23.890907644742832
18	21.465258332830075	26.380335566812146	27.67608714544322	24.478318954914563
19	22.837455866822012	26.32127038742928	29.021035157774733	21.820238587973975
20	22.685005840235316	26.388289481242754	28.86269334272092	22.064011335801013
21	22.94203511211337	26.496109210191026	27.61702196606035	22.94483371163525
22	24.002704330906408	26.028448500613482	27.132127775216485	22.836719393263625
23	23.103175526689064	26.51054409193547	27.896440034113457	22.489840347262014
24	21.624778505577314	27.226101801267028	26.921790926940346	24.227328766215305
25	23.561703964142573	26.42364021204547	28.176594575724952	21.83806124808701
26	22.57556586945859	26.87406744035669	27.850484084069933	22.699882606114794
27	21.950447113097297	27.31712993308401	27.498155133736233	23.234267820082454
28	22.132797966154623	27.206658899325536	28.90364127256739	21.75690186195245
29	23.06811938530971	26.902495319710535	27.899975107193725	22.12941018778603
30	22.1516516892494	28.171439260816218	27.625417764625997	22.051491285308384
31	23.351514410578115	26.778178583054334	27.927813807700865	21.94249319866669
32	21.421069919326683	27.34496863359115	27.735152324826085	23.498809122256084
33	21.40634044815889	27.458827445718214	28.18233906948039	22.952493036642505
34	22.539773254520842	26.14716803822592	27.96537395917874	23.34768474807449
35	22.812857649971793	27.647806560801047	28.14507350742586	21.394262281801296
36	22.460970583773133	27.633666268479963	26.672126390646195	23.23323675710071
37	22.53064098239681	26.64443498485074	27.887749646124455	22.937174386627998
38	21.929678558750705	27.5759267415022	28.062293879462846	22.432100820284248
39	21.22281123740814	27.124173860785877	28.689916645922665	22.963098255883317
40	22.07196525023162	26.630441987241333	29.32667168450651	21.970921078020535
41	21.36598169715913	26.190325388747564	29.216347945459713	23.227344968633588
42	20.49488077229563	26.8064591676965	29.327408158064898	23.371251901942962
43	21.279961585539194	25.708377092137262	29.817752253240855	23.193909069082693
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	13.0
1	25.5
2	38.0
3	155.5
4	273.0
5	273.0
6	440.5
7	608.0
8	589.5
9	571.0
10	788.5
11	1006.0
12	1006.0
13	2252.0
14	3498.0
15	6322.5
16	9147.0
17	7687.5
18	6228.0
19	6228.0
20	6772.5
21	7317.0
22	5294.5
23	3272.0
24	3654.5
25	4037.0
26	4037.0
27	5167.5
28	6298.0
29	8759.0
30	11220.0
31	14078.0
32	16936.0
33	16936.0
34	22347.5
35	27759.0
36	31075.0
37	34391.0
38	37306.0
39	40221.0
40	40221.0
41	46429.5
42	52638.0
43	57535.5
44	62433.0
45	65682.0
46	68931.0
47	68931.0
48	71173.0
49	73415.0
50	68283.0
51	63151.0
52	58270.0
53	53389.0
54	53389.0
55	48949.0
56	44509.0
57	37235.5
58	29962.0
59	25790.5
60	21619.0
61	21619.0
62	18517.0
63	15415.0
64	11776.0
65	8137.0
66	6581.5
67	5026.0
68	5026.0
69	3916.0
70	2806.0
71	2151.5
72	1497.0
73	1295.5
74	1094.0
75	1094.0
76	949.0
77	804.0
78	641.5
79	479.0
80	392.5
81	306.0
82	306.0
83	263.5
84	221.0
85	164.5
86	108.0
87	103.0
88	98.0
89	98.0
90	65.0
91	32.0
92	20.0
93	8.0
94	4.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	678911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	22.244114001202618
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.45979816271318	15.005834408295108
2	11.995817147989454	5.336726483549173
3	4.894891610564447	3.266475810267775
4	2.7902678577219024	2.482681452842296
5	1.784333196297281	1.9845455517283481
6	1.3163343701778563	1.756841507636249
7	1.0235637388226633	1.5937787945707955
8	0.7587179798377679	1.3501607390618766
9	0.7074967504063452	1.4163874534367222
>10	5.805795731820272	27.141208458184153
>50	0.9040759817920493	14.08921835451312
>100	0.5469593311282295	21.30252134099439
>500	0.009292998344411666	1.2732137566255501
>1k	0.001991356788088214	1.242501885887047
>5k	6.637855960294047E-4	0.7579040024073929
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	5133	0.7560637550430027	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	3906	0.5753331438141376	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	3142	0.46279998409217116	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1367	0.2013518708637804	No Hit
CCCATAGTAGGCCTAAAAGCAGCCACCAATTAAGAAAGCGTTC	884	0.13020852512332248	No Hit
GATTTAGAGGGTTCTGTGGGCAAATTTAAAGTTGAACTAAGAT	715	0.10531571884974614	No Hit
GAACAGTGGTATCAACGCAAAAAAAAAAAAAAAAAAAAAAAAA	693	0.10207523519283088	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	1.4729471167796663E-4	0.0
3	0.0	0.0	0.0	1.4729471167796663E-4	0.0
4	0.0	0.0	0.0	1.4729471167796663E-4	0.0
5	0.0	0.0	0.0	1.4729471167796663E-4	0.0
6	0.0	0.0	0.0	1.4729471167796663E-4	0.0
7	0.0	0.0	0.0	1.4729471167796663E-4	0.0
8	0.0	0.0	0.0	1.4729471167796663E-4	0.0
9	0.0	0.0	0.0	1.4729471167796663E-4	0.0
10	0.0	0.0	0.0	1.4729471167796663E-4	0.0
11	0.0	0.0	0.0	1.4729471167796663E-4	0.0
12	0.0	0.0	0.0	1.4729471167796663E-4	0.0
13	0.0	0.0	0.0	1.4729471167796663E-4	0.0
14	0.0	0.0	0.0	2.9458942335593326E-4	0.0
15	0.0	1.4729471167796663E-4	0.0	8.837682700677998E-4	0.0
16	0.0	1.4729471167796663E-4	0.0	0.0014729471167796663	0.0
17	0.0	1.4729471167796663E-4	0.0	0.002356715386847466	0.0
18	0.0	1.4729471167796663E-4	0.0	0.0025040100985254327	0.0
19	0.0	1.4729471167796663E-4	0.0	0.003093188945237299	0.0
20	0.0	1.4729471167796663E-4	0.0	0.003535073080271199	0.0
21	0.0	1.4729471167796663E-4	0.0	0.004418841350338999	0.0
22	0.0	1.4729471167796663E-4	0.0	0.0069228514488644316	0.0
23	0.0	1.4729471167796663E-4	0.0	0.008984977412355964	0.0
24	0.0	1.4729471167796663E-4	0.0	0.01266734520430513	0.0
25	0.0	1.4729471167796663E-4	0.0	0.013698408186050896	0.0
26	0.0	1.4729471167796663E-4	0.0	0.015465944726186495	0.0
27	0.0	1.4729471167796663E-4	0.0	0.020179375499881427	0.0
28	0.0	1.4729471167796663E-4	0.0	0.030490005317339093	0.0
29	0.0	1.4729471167796663E-4	0.0	0.05479363274420358	0.0
30	0.0	1.4729471167796663E-4	0.0	0.10708325538988174	0.0
31	0.0	1.4729471167796663E-4	0.0	0.25820762957147547	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTCTATA	20	0.0018414049	37.0	2
CTTAAGG	25	0.005494779	29.599998	19
AACCGCG	25	0.005494779	29.599998	35
TCACATT	25	0.005494779	29.599998	26
ATAAGGT	50	2.7180067E-7	29.599998	3
GCGGATC	45	4.00508E-6	28.777777	33
TTGTGCA	40	5.9366008E-5	27.750002	19
ACTGTGC	40	5.9366008E-5	27.750002	8
TGCGGAT	50	9.083933E-6	25.899998	32
CGCCGAA	80	9.767973E-10	25.437502	33
TTAGAGT	60	1.3360604E-6	24.666668	4
GAGGCAC	110	3.6379788E-12	23.545454	7
CGTCTAA	90	3.8216967E-9	22.61111	1
GCCGAAT	90	3.8216967E-9	22.61111	34
TTAGGTT	115	5.456968E-12	22.52174	4
GTGATCG	50	2.700524E-4	22.199999	8
GTTGTGC	50	2.700524E-4	22.199999	18
CTCGCTA	50	2.700524E-4	22.199999	13
GAGCATG	50	2.700524E-4	22.199999	7
GCCGTTT	50	2.700524E-4	22.199999	12
>>END_MODULE