FastQCFastQC Report
Fri 10 Feb 2017
ERR1630223.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630223.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051099
Sequences flagged as poor quality0
Sequence length43
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC66100.32226625823521926No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT61800.3013018874271793No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT56080.2734143988174145No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT54410.26527242224778036No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA47100.22963299187411237No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA46740.22787783524832297No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT41540.20252557287580952No Hit
GTATGCAGCTGATCTCCGTAGATACATCAACATGCTGACCAGG41220.2009654336528856No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA34400.16771496646431985No Hit
AGTGTACCCAGGGGACAATGCCACACCAGAGCAGATGGCCCAG33090.16132814652047514No Hit
GCTGCATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTG32060.15630644839668878No Hit
CCTCTGCCTCTCCCTGCTGCTCCTGTCCACCTGCGTGGCTCTG31990.1559651679416742No Hit
GGGTACACTGGCTCCAGTGGGGCTCCCTGGGCACCCAGCAGTG31080.15152852202648434No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA30690.14962710234854584No Hit
CTGTTACTACAGCCACTGCTGGGTGCCCAGGGAGCCCCACTGG28540.13914491694452583No Hit
GTCTAGTGCCCATTTACTCTGGACTCCGGATGGCTGCCGCACG28090.1369509711622891No Hit
GGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTAGGAGACA27520.13417197317145588No Hit
CCCTGGGGACAGCAGCATGCGGGGACCCCCACTCCGAGAAGGC25680.12520117263964342No Hit
ATACTGGGCCATCTGCTCTGGTGTGGCATTGTCCCCTGGGTAC24420.11905812444938055No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24280.11837556353935134No Hit
GTCGTAGGAGACAGAAGGTGGCATTATAAGTCCAGCGGGCTGA23880.11642538951069645No Hit
TATCTACGGAGATCAGCTGCATACTGGGCCATCTGCTCTGGTG22750.11091614787974641No Hit
CCGTAGATACATCAACATGCTGACCAGGCCTAGGTATGGGAAA22680.11057486742473181No Hit
ACTCTGGACTCCGGATGGCTGCCGCACGCCTCTGCCTCTCCCT22680.11057486742473181No Hit
CTGCTGTCCCCAGGGAGCTCAGCCCGCTGGACTTATAATGCCA22290.1086734477467933No Hit
GCCCAGTATGCAGCTGATCTCCGTAGATACATCAACATGCTGA22140.10794213248604771No Hit
GCTTTGGCCAGAGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGG21790.10623573021097471No Hit
CACCAGAGCAGATGGCCCAGTATGCAGCTGATCTCCGTAGATA21640.10550441495022912No Hit
AGCCAAGGGTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATG20660.10072648858002467No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA19000.022.5894741
CGAATCA450.003826816620.55555515
TATGCCG500.007036719518.4999985
GCTTTAT14150.018.434631
ACGAGCG1352.2220775E-816.4444455
TTATTGA16050.016.3676014
CGAGCCG1606.311893E-1016.187515
CGAGCGT1504.6766218E-916.0333336
TATTGAG16050.015.9065435
ACCGTCG1059.350169E-615.8571428
TCGCGGT1302.5916597E-715.65384617
TCTAGCG957.0639915E-515.57894828
GTTCGCG957.0639915E-515.57894830
CGAATTA2150.015.48837215
TACGTTA1201.9372055E-615.41666619
TACCGTC1201.9372055E-615.4166667
CGGTCGA1353.9762563E-715.07407520
GTATCAA28600.015.0069932
CTTATAC7800.014.94230837
TTTATTG18400.014.7798923
Produced by FastQC (version 0.11.5)