FastQCFastQC Report
Fri 10 Feb 2017
ERR1630222.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630222.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences768683
Sequences flagged as poor quality0
Sequence length43
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT12970.16873015274176742No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT11530.1499968127303453No Hit
GCTTAGGACACAGAGCACATCAAAAGTTCCCAAAGAGGGCTTG9900.12879171257852717No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT9460.12306763646392595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGATC250.00549522629.5999988
ACGCGCT250.00549522629.59999811
TGCGTTA400.001930608123.12500237
CGCAAGA1200.023.1249982
GGTATCA6650.021.4210531
CAGTACT705.0982944E-621.1428594
GACGGAC1251.8189894E-1120.727
CGCGCTG450.003824673120.55555512
CACCGAT450.003824673120.5555557
TACGACG1450.020.4137945
CGGACCA1403.6379788E-1219.821439
ACGTGTC759.259222E-619.7333348
CCTATAC1153.0449883E-919.3043483
GACGGTA1457.2759576E-1219.137938
GTAAACG1457.2759576E-1219.1379327
CTAGACT609.233024E-418.54
CGACGGT1501.2732926E-1118.57
TAGACTG1103.846617E-818.55
TTAGACT609.233024E-418.54
AAGACGG1601.8189894E-1218.55
Produced by FastQC (version 0.11.5)