FastQCFastQC Report
Fri 10 Feb 2017
ERR1630221.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameERR1630221.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2473384
Sequences flagged as poor quality0
Sequence length43
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT127530.5156093837430824No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT124660.5040058478586422No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT96110.38857694559356737No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58810.23777140953446774No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA46300.18719293081866786No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA40820.16503705045395298No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT40000.16172175448697007No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC35590.14389193105478162No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA28910.11688439805545763No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA25840.10447225339858268No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTGCCG451.3238145E-424.66666629
ACGCGAA400.001931812823.12519
CGCGAAT400.001931812823.12520
CGACGAG806.966784E-720.812524
CGGTATA450.00382703620.55555526
TGCGACG609.2409196E-418.522
GACGCGA609.2409196E-418.518
GGTATCA50950.018.1550541
CGCAATA1800.017.47222136
GCTTTAT14350.016.5017411
CGCACTA803.3845365E-416.187529
TACCGGC1504.6784407E-916.0333337
GATCGAT700.002593625815.85714311
CGCCGTA1557.219569E-915.51612925
TCTGTCG2150.015.4883748
CTTATAC19250.015.47272837
GTGCGTA1101.4531348E-515.13636415
TCTAGCG2151.2732926E-1114.62790828
ACCTAGG12500.014.3559991
TAGTCGG1551.2118107E-714.3225830
Produced by FastQC (version 0.11.5)