##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	ERR1630221.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	2473384
Sequences flagged as poor quality	0
Sequence length	43
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.908740413943004	33.0	31.0	34.0	30.0	34.0
2	32.090804743622506	33.0	31.0	34.0	30.0	34.0
3	32.22969340789784	34.0	31.0	34.0	30.0	34.0
4	35.86706633502926	37.0	35.0	37.0	35.0	37.0
5	35.7926464309626	37.0	35.0	37.0	35.0	37.0
6	35.86439913899338	37.0	35.0	37.0	35.0	37.0
7	35.839862310098226	37.0	35.0	37.0	35.0	37.0
8	35.833735077125105	37.0	35.0	37.0	35.0	37.0
9	37.53260755305282	39.0	37.0	39.0	35.0	39.0
10	37.47153737551468	39.0	37.0	39.0	35.0	39.0
11	37.539680049680925	39.0	37.0	39.0	35.0	39.0
12	37.440307691810084	39.0	37.0	39.0	35.0	39.0
13	37.477566362521955	39.0	37.0	39.0	35.0	39.0
14	38.69473886788303	40.0	38.0	41.0	35.0	41.0
15	38.691452681831855	40.0	38.0	41.0	35.0	41.0
16	38.66100249698389	40.0	38.0	41.0	34.0	41.0
17	38.662468100383926	40.0	38.0	41.0	35.0	41.0
18	38.65582901805785	40.0	38.0	41.0	34.0	41.0
19	38.66962186219366	40.0	38.0	41.0	34.0	41.0
20	38.64167432149638	40.0	38.0	41.0	34.0	41.0
21	38.599865204917634	40.0	38.0	41.0	34.0	41.0
22	38.56469314914304	40.0	38.0	41.0	34.0	41.0
23	38.52715631701345	40.0	38.0	41.0	34.0	41.0
24	38.506027774094115	40.0	38.0	41.0	34.0	41.0
25	38.47903317883515	40.0	38.0	41.0	34.0	41.0
26	38.330180432961484	40.0	38.0	41.0	34.0	41.0
27	38.23105995672326	40.0	38.0	41.0	34.0	41.0
28	38.154725671387865	40.0	38.0	41.0	34.0	41.0
29	38.08843956296313	40.0	38.0	41.0	33.0	41.0
30	38.02815656606495	40.0	37.0	41.0	33.0	41.0
31	37.90248986813208	40.0	37.0	41.0	33.0	41.0
32	37.80603294919026	40.0	37.0	41.0	33.0	41.0
33	37.72693403046191	40.0	37.0	41.0	33.0	41.0
34	37.63982058588557	40.0	37.0	41.0	33.0	41.0
35	37.543165153490115	40.0	37.0	41.0	33.0	41.0
36	37.43577422672743	40.0	36.0	41.0	32.0	41.0
37	37.365575260452886	40.0	36.0	41.0	32.0	41.0
38	37.26097726839019	40.0	36.0	41.0	31.0	41.0
39	37.14855679506296	39.0	36.0	41.0	31.0	41.0
40	37.02870439850828	39.0	36.0	41.0	31.0	41.0
41	36.910768809048655	39.0	36.0	41.0	31.0	41.0
42	36.834481018717675	39.0	35.0	41.0	31.0	41.0
43	35.69795713079732	38.0	35.0	40.0	28.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	9.0
10	7.0
11	4.0
12	2.0
13	4.0
14	2.0
15	6.0
16	15.0
17	37.0
18	69.0
19	127.0
20	303.0
21	619.0
22	1106.0
23	1917.0
24	3332.0
25	5285.0
26	8323.0
27	12393.0
28	18390.0
29	25626.0
30	35243.0
31	47143.0
32	62314.0
33	81683.0
34	109464.0
35	148687.0
36	208630.0
37	323340.0
38	577650.0
39	801651.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.84732981211167	19.47392721874161	13.022320836554291	24.656422132592432
2	18.401873708247486	21.125348914685304	34.62131233969331	25.8514650373739
3	20.373100173689167	23.09038952301786	29.50382957114625	27.032680732146723
4	14.485862284222751	15.364820019859431	34.89696707021635	35.25235062570147
5	14.45388180727295	36.93680398999913	33.98081333104766	14.628500871680256
6	34.39793416630819	34.85285746168003	15.638897963276225	15.110310408735561
7	29.085940557551922	30.874744883932298	21.223433158781653	18.81588139973413
8	27.99589550187112	32.757024384406144	20.01565466583434	19.231425447888398
9	26.74283491766746	14.504177272918398	20.52895951457598	38.22402829483816
10	17.954510904897905	27.03506612802541	32.442435141490364	22.56798782558632
11	34.50236598926815	22.668983061263432	21.146413173207236	21.682237776261186
12	22.28533862918172	26.457072577488976	28.61743263480317	22.640156158526132
13	30.768453260795738	19.97348571835186	24.814222134533093	24.44383888631931
14	22.84679613032186	21.463953838142398	25.7512379800306	29.93801205150514
15	25.4185763310509	28.21001510481187	22.659279755994216	23.712128808143014
16	23.964536036458554	26.93669887085871	24.777632587580417	24.321132505102323
17	23.093340945037244	26.763130997855573	25.999723455799828	24.14380460130736
18	22.638579371419883	25.288592470881998	27.848405261779003	24.224422895919112
19	23.97096447619941	25.479464571615246	28.111162682381707	22.438408269803638
20	24.843978937358695	25.13758478262979	26.92917880927507	23.08925747073645
21	24.5739844682427	25.151897157901885	26.855393258790382	23.41872511506503
22	24.390632429093095	25.404223525340182	27.4050855022916	22.800058543275124
23	23.932151255122534	25.358051964434154	26.695733456673125	24.014063323770188
24	23.3734834542473	25.96244659139058	27.679082584831143	22.984987369530973
25	23.703557555155204	26.09930362612518	26.764707784961818	23.4324310337578
26	23.63603872265689	26.12833268105559	26.926389109010167	23.30923948727735
27	23.66122688591824	25.065659032321708	27.314238306708543	23.958875775051506
28	23.127504665672618	25.807759733223794	27.19925413926831	23.865481461835284
29	23.07648145213198	26.30323475853325	27.5875076413529	23.032776147981874
30	23.61921966019025	26.295229531686143	26.681542372716894	23.404008435406716
31	22.92276492449211	26.941024927791236	27.172529619339336	22.963680528377317
32	22.584038709719152	26.162092097304747	27.220560980421965	24.033308212554136
33	22.070774291416132	26.31350408994317	27.765401571288567	23.85032004735213
34	23.72890744017104	24.861121443334312	27.666104414033565	23.74386670246108
35	22.901134639829483	25.447322372910957	27.58560741073768	24.065935576521884
36	22.411481597681558	26.341967118732878	27.70629227002358	23.540259013561986
37	22.971726185663044	25.212340663641392	28.080354688151942	23.735578462543625
38	23.081696978714184	25.43960015913421	27.879981434342582	23.59872142780903
39	22.76791634457084	24.76566517774838	28.68337468019523	23.78304379748555
40	21.781737085709295	24.53359446005958	29.377039715628467	24.30762873860266
41	21.189835464286986	24.918856109686164	29.635026344473808	24.256282081553046
42	21.409372746003047	24.895204303092445	29.872474310499296	23.822948640405208
43	21.50228189395581	24.900621981867758	29.515756550539667	24.081339573636768
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2085.0
1	1594.5
2	1104.0
3	1973.0
4	2842.0
5	2842.0
6	3654.5
7	4467.0
8	4034.0
9	3601.0
10	5123.0
11	6645.0
12	6645.0
13	11594.5
14	16544.0
15	23102.5
16	29661.0
17	28193.0
18	26725.0
19	26725.0
20	31148.5
21	35572.0
22	32961.0
23	30350.0
24	35540.0
25	40730.0
26	40730.0
27	46404.0
28	52078.0
29	58843.0
30	65608.0
31	72349.5
32	79091.0
33	79091.0
34	86475.0
35	93859.0
36	101710.0
37	109561.0
38	114867.5
39	120174.0
40	120174.0
41	125259.0
42	130344.0
43	132276.5
44	134209.0
45	139256.5
46	144304.0
47	144304.0
48	153722.5
49	163141.0
50	162146.0
51	161151.0
52	163286.0
53	165421.0
54	165421.0
55	161201.0
56	156981.0
57	153812.0
58	150643.0
59	147403.5
60	144164.0
61	144164.0
62	133325.5
63	122487.0
64	111173.5
65	99860.0
66	84560.5
67	69261.0
68	69261.0
69	60118.5
70	50976.0
71	42263.0
72	33550.0
73	23033.0
74	12516.0
75	12516.0
76	9292.0
77	6068.0
78	4601.0
79	3134.0
80	2476.0
81	1818.0
82	1818.0
83	1397.5
84	977.0
85	830.5
86	684.0
87	592.0
88	500.0
89	500.0
90	438.5
91	377.0
92	228.5
93	80.0
94	55.0
95	30.0
96	30.0
97	19.0
98	8.0
99	5.5
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
43	2473384.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	54.39070888036797
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.41569055248863	43.19475705373798
2	12.628253198439014	13.73719286767744
3	3.7001844931220225	6.037669727071555
4	1.4598829061975933	3.1761626460167536
5	0.7232790847426691	1.9669831068748755
6	0.4128079873917436	1.3471751439428965
7	0.2684028789474796	1.0219035996039505
8	0.1998046996154649	0.8694015399771297
9	0.14587459163502464	0.7140800201996867
>10	0.8769723724698089	9.11451530541064
>50	0.09416612661311825	3.5653006659929223
>100	0.06226732841727819	6.919200724130278
>500	0.008176741264712448	2.9693993661947236
>1k	0.00393970260936082	3.7202801056551875
>5k	1.486680229947479E-4	0.6263538884195157
>10k+	1.486680229947479E-4	1.0196242390944854
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT	12753	0.5156093837430824	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTT	12466	0.5040058478586422	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTT	9611	0.38857694559356737	No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5881	0.23777140953446774	No Hit
GTGTGGGAGCAGGGAGCAAGCTTTGGCCAGAGCCAAGGGTGCA	4630	0.18719293081866786	No Hit
GCTTTATTGAGCCTGTGTGGGAGCAGGGAGCAAGCTTTGGCCA	4082	0.16503705045395298	No Hit
GGCCTAGGTATGGGAAAAGACACAAAGAGGACACGCTGGCCTT	4000	0.16172175448697007	No Hit
ACCTAGGCCTGGTCAGCATGTTGATGTATCTACGGAGATCAGC	3559	0.14389193105478162	No Hit
GCTCATGGAGTCGTAGGAGACAGAAGGTGGCATTATAAGTCCA	2891	0.11688439805545763	No Hit
GTGCAGAGGGGAGAGCTGGGCTGGCGCTGCTCATGGAGTCGTA	2584	0.10447225339858268	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	4.043043862174252E-5	0.0
8	0.0	0.0	0.0	4.043043862174252E-5	0.0
9	0.0	0.0	0.0	4.043043862174252E-5	0.0
10	0.0	0.0	0.0	4.043043862174252E-5	0.0
11	0.0	0.0	0.0	4.043043862174252E-5	0.0
12	0.0	0.0	0.0	8.086087724348504E-5	0.0
13	0.0	0.0	0.0	1.2129131586522756E-4	0.0
14	0.0	0.0	0.0	3.6387394759568266E-4	0.0
15	0.0	0.0	0.0	6.064565793261377E-4	0.0
16	0.0	0.0	0.0	9.703305269218205E-4	0.0
17	0.0	0.0	0.0	0.0016980784221131858	0.0
18	0.0	0.0	0.0	0.001859800176600156	0.0
19	0.0	0.0	0.0	0.0021832436855740963	0.0
20	0.0	0.0	0.0	0.0025471176331697785	0.0
21	0.0	0.0	0.0	0.0035983090373350843	0.0
22	0.0	0.0	0.0	0.005538970091178725	0.0
23	0.0	0.0	0.0	0.00784350509261805	0.0
24	0.0	0.0	0.0	0.011967409832035786	0.0
25	0.0	0.0	0.0	0.014312375272096852	0.0
26	0.0	0.0	0.0	0.019366180099814667	0.0
27	0.0	0.0	0.0	0.04047086906036426	0.0
28	0.0	0.0	0.0	0.09860983979843001	0.0
29	0.0	0.0	0.0	0.1954003098588816	0.0
30	0.0	0.0	0.0	0.3119208339667435	0.0
31	0.0	0.0	0.0	0.6476147658430716	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTGCCG	45	1.3238145E-4	24.666666	29
ACGCGAA	40	0.0019318128	23.125	19
CGCGAAT	40	0.0019318128	23.125	20
CGACGAG	80	6.966784E-7	20.8125	24
CGGTATA	45	0.003827036	20.555555	26
TGCGACG	60	9.2409196E-4	18.5	22
GACGCGA	60	9.2409196E-4	18.5	18
GGTATCA	5095	0.0	18.155054	1
CGCAATA	180	0.0	17.472221	36
GCTTTAT	1435	0.0	16.501741	1
CGCACTA	80	3.3845365E-4	16.1875	29
TACCGGC	150	4.6784407E-9	16.033333	7
GATCGAT	70	0.0025936258	15.857143	11
CGCCGTA	155	7.219569E-9	15.516129	25
TCTGTCG	215	0.0	15.488374	8
CTTATAC	1925	0.0	15.472728	37
GTGCGTA	110	1.4531348E-5	15.136364	15
TCTAGCG	215	1.2732926E-11	14.627908	28
ACCTAGG	1250	0.0	14.355999	1
TAGTCGG	155	1.2118107E-7	14.32258	30
>>END_MODULE